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  • 1
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Short Overview of PINK1/Parkin-mediated Mitophagy -- Induction of PINK1/Parkin-Mediated Mitophagy -- Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells -- The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy -- Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells -- Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila -- Assessment of Mitophagy in iPS Cell-derived Neurons -- Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting -- Mitopho8∆60 Assay as a Tool to Quantitatively Measure Mitophagy Activity -- Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants -- Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy -- Exploring Micrornas on Nix-Dependent Mitophagy -- Monitoring of Atg5-Independent Mitophagy -- Monitoring of Paternal Mitochondrial Degradation In Caenorhabditis Elegans -- Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells -- Monitoring Mitophagy during Ageing in Caenorhabditis Elegans -- Iron-Starvation Induced Mitophagy in Yeast
    Abstract: This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson’s disease, especially young onset Parkinson’s disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy
    Pages: XI, 180 p. : online resource.
    ISBN: 9781493977505
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  • 2
    Keywords: Medicine ; Immunology ; Neurosciences ; Pharmacology ; Endocrinology ; Biochemistry ; Biomedicine ; Pharmacology/Toxicology ; Biochemistry, general ; Neurosciences ; Immunology ; Endocrinology ; Springer eBooks
    Description / Table of Contents: Part 1: New Analytical tools -- Histamine and histamine receptors: State of the art -- Mass-spectrometric methods for the detection of histamine and histamine metabolites -- Fluorescent ligands and radioligands for the analysis of histamine receptors -- Molecular analysis of histamine receptors: Structural analysis of the histamine H1-receptor -- Molecular modelling approaches for the analysis of histamine receptors -- Cellular analysis of histamine receptors: Histamine release from human mast cells and basophils -- Histamine H2-receptor in blood cells: A suitable target for the treatment of acute myeloid leukemia -- Histamine H1-receptor-regulated gene expression and drug action of antihistamines -- Histamine metabolism and transport: Analysis of the role of histamine and genetically engineered mice in a human disease model -- Histamine clearance through polyspecific transporters in the brain -- Histamine decarboxylase deficiency as a cause for Tourette syndrome -- Histamine food poisoning -- Integrative role of histamine in major organ systems: Pharmacological implications: Regulation of the cardiovascular system by histamine -- Regulation of eating behavior by histamine -- Role of the histamine H3-receptor in the CNS -- Role of the histamine H4-receptor in bronchial asthma -- The histamine H4-receptor in pruritic skin disease -- Histamine in allergy and antihistamines
    Abstract: This book provides a comprehensive overview on current histamine and histamine receptor research in context of human health and disease and reflect the multidisciplinary nature of the field. While the editors realize that it is almost impossible to cover the field completely within the constraints of a single HEP volume, nonetheless, all important aspects will be covered in one way or the other. An overarching introductory chapter will link the individual chapters and provide an overview on the field. This chapter will also link the book to the previous HEP volume on histamine receptors and the recent HEP volume on the pharmacology of itch. Great attention will be paid to complementation of existing literature while avoiding undue duplication. The book will cover new methods for analysis of histamine and histamine metabolites, development of methods for histamine receptor analysis, signal transduction, histamine release, regulation of immune cells by histamine, histamine metabolism and associated diseases, regulation of major organ systems by histamine and development of new drugs and experimental tools for the study of histamine receptors
    Pages: VIII, 363 p. 90 illus., 30 illus. in color. : online resource.
    ISBN: 9783319581941
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  • 3
    Publication Date: 2018-03-13
    Description: Sepsis-associated encephalopathy (SAE), characterized as diffuse brain dysfunction and neurological manifestations secondary to sepsis, is a common complication in critically ill patients and can give rise to poor outcome, but understanding the molecular basis of this disorder remains a major challenge. Given the emerging role of G protein-coupled receptor 2 (GRK2), first identified as a G protein-coupled receptor (GPCR) regulator, in the regulation of non-GPCR-related molecules contributing to diverse cellular functions and pathology, including inflammation, we tested the hypothesis that GRK2 may be linked to the neuropathogenesis of SAE. When mouse MG6 microglial cells were challenged with lipopolysaccharide (LPS), GRK2 cytosolic expression was highly upregulated. The ablation of GRK2 by small interfering RNAs (siRNAs) prevented an increase in intracellular reactive oxygen species generation in LPS-stimulated MG6 cells. Furthermore, the LPS-induced upregulation of inducible nitric-oxide synthase expression and increase in nitric oxide production were negated by GRK2 inhibitor or siRNAs. However, GRK2 inhibition was without effect on overproduction of tumor necrosis factor-α, interleukin (IL)-6, and IL-1β in LPS-stimulated MG cells. In mice with cecal ligation and puncture-induced sepsis, treatment with GRK2 inhibitor reduced high levels of oxidative and nitrosative stress in the mice brains, where GRK2 expression was upregulated, alleviated neurohistological damage observed in cerebral cortex sections, and conferred a significant survival advantage to CLP mice. Altogether, these results uncover the novel role for GRK2 in regulating cellular oxidative and nitrosative stress during inflammation and suggest that GRK2 may have a potential as an intriguing therapeutic target to prevent or treat SAE. This article is protected by copyright. All rights reserved.
    Print ISSN: 0022-3042
    Electronic ISSN: 1471-4159
    Topics: Medicine
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters A 115 (1986), S. 207-212 
    ISSN: 0375-9601
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Abstract: We study a kind of “restoration of isotropy” on the pre-Sierpiński. Let and be the effective resistances in the x and y directions, respectively, of the Sierpiński at the n th stage of its construction, if it is made of anisotropic material whose anisotropy is parametrized by the ratio of resistances for a unit square: . We prove that isotropy is weakly restored asymptotically in the sense that for all sufficiently large n the ratio is bounded by positive constants independent of r. The ratio decays exponentially fast when r≫ 1. Furthermore, it is proved that the effective resistances asymptotically grow exponentially with an exponent equal to that found by Barlow and Bass for the isotropic case r = 1.
    Type of Medium: Electronic Resource
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  • 6
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Inc
    Birth 26 (1999), S. 0 
    ISSN: 1523-536X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Although the frequency of twin births has increased in Japan, little information is available about breastfeeding twins. An intervention of breastfeeding support was implemented to promote breastfeeding for mothers of twins and to compare the factors related to breastfed and formula-fed twins. Methods: Eleven Japanese twins-mother pairs received breastfeeding support according to a breastfeeding protocol for twins, and a control group of seven pairs received routine care. Perinatal data were obtained from clinical records for analysis. Results: No significant differences were evident between the two groups in terms of maternal age, gestational age, birthweight, the age at which breastfeeding was initiated, amount of supplementary feeding in hospital, infants' age at discharge, or duration of mothers' hospital stay. At age 3 months, 13 infants in the intervention group were being exclusively breastfed and 9 were almost exclusively breastfed. In the control group 4 infants were almost exclusively and 10 were exclusively formula-fed. The mothers in the intervention group breastfed and expressed milk significantly more frequently than the mothers in the control group during their hospital stay. Conclusions: Mothers of twins face many obstacles in breastfeeding, and health care professionals should offer individualized care and support.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0009-8981
    Keywords: Alkaline phosphatase ; Enzyme-immunoglobulin complex ; Polyacrylamide gel electrophoresis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Eleven isolates of slow-growing oligotrophic bacteria from grassland soil were found to be closely related by partial 16S rRNA sequence similarity and many common taxonomic traits. Analysis of full 16S rRNA gene sequences of four representative isolates and Agromonas oligotrophica S58 indicated that they were more closely related to Bradyrhizobium japonicum, a symbiotic nitrogen-fixing bacterium, (similarity values: 98.1–98.8%) than other strains such as Bradyrhizobium elkanii, Nitrobacter spp., Rhodopseudomonas palustris, and Afipia spp. This result was supported by analysis of phenotypic traits and DNA-DNA hybridization analysis. No strain showed hybridization to nodD1YABC of B. japonicum, and only strain G14130 exhibited hybridization to nifDK- and hupSL-specific DNA. These latter genotypes are involved in the phenotypes of nodulation and nitrogen fixation under microaerobic conditions. These results suggest that the isolates possess a unique phylogenetic position since they are closely related to B. japonicum though they do not have characteristics of symbiotic nitrogen fixation.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as main hopanoid a bacteriohopanetetrol carbapseudopentose ether, accompanied by significant amounts of its novel Δ6 unsaturated homologue. Unsaturated hopanoids represent an extremely rare feature in soil bacteria and the only known indication for a catabolism of this pentacyclic carbon skeleton in bacteria.
    Type of Medium: Electronic Resource
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