AIP Digital Archive
Electrical Engineering, Measurement and Control Technology
A sample-scanning confocal optical microscope for single-molecule imaging and spectroscopy working at superfluid helium temperature, liquid nitrogen, and room temperature is described. An optical resolution of 800 nm full width at half maximum as well as a detection efficiency of (approximate)3.5% are achieved. The sample scanner features an exceptionally large scan range of 23 μm at 1.8 K. A position sensor allows for continuous observation of the scanner motion and for a correction of piezoelectric hysteresis and creep at 77 K and at room temperature. Coarse positioning of the sample in x-y is achieved by an inertia drive with high reproducibility and nanometer precision. We demonstrate combined high–resolution confocal imaging and spectroscopy of single molecules at 1.8 K. © 2000 American Institute of Physics.
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