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  • 1
    Keywords: Microbiology ; Microbial genetics ; Microbial genomics ; Microbiology ; Microbial Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Experimental Set Up and Data Analysis Considerations for DNA- and RNA-SIP Experiments in the Omics Era -- DNA-Based Stable Isotope Probing -- RNA Stable Isotope Probing (RNA-SIP) -- Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples -- SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity -- Chip-SIP: Stable Isotope Probing Analyzed with rRNA-Targeted Microarrays and NanoSIMS -- Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures -- Stable Isotope-Labeled Single-Cell Raman Spectroscopy Revealing Function and Activity of Environmental Microbes -- Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High Resolution SIP -- Stable Isotope Probing of Microorganisms in Environmental Samples with H218O -- Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing -- Profiling of Active Microorganisms by Stable Isotope Probing-Metagenomics -- Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing -- Stable Isotope Probing Techniques and Methodological Considerations Using 15N -- DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea -- Method Development for DNA and Proteome SIP Analysis of Activated Sludge for Anaerobic Dichloromethane Biodegradation -- RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment -- Stable Isotope Probing of Microbiota Structure and Function in the Plant Rhizosphere
    Abstract: This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study
    Pages: XI, 247 p. 43 illus., 35 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493997213
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  • 2
    Call number: YY Diss Herr/Mag
    Keywords: DKFZ-publications / academic dissertations
    Pages: xii, 150 p. : ill.
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    YY Diss Herr/Mag departmental collection or stack – please contact the library
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  • 3
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    New York, NY : Springer Science+Business Media, LLC
    Keywords: Medicine ; Neurosciences ; Computer science ; Information Systems ; Artificial intelligence ; Biomedicine ; Neurosciences ; Computation by Abstract Devices ; Artificial Intelligence (incl. Robotics) ; Information Systems Applications (incl.Internet) ; Signal, Image and Speech Processing ; Springer eBooks
    Pages: : digital
    ISBN: 9781461401643
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  • 4
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    Heidelberg
    Call number: YY Diss Hern/Mag
    Keywords: DKFZ-publications / academic dissertations
    Notes: Thesis (PhD) -- Ruprecht-Karls-Universität, Heidelberg, 1983.
    Pages: 112 p.
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    YY Diss Hern/Mag departmental collection or stack – please contact the library
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  • 5
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    Amsterdam : Elsevier Academic Press
    Call number: QH405:35(2)
    Keywords: Biometry / methods ; Research Design
    Description / Table of Contents: Data and numbers -- Descriptive methods -- Probability and life tables -- Probability distributions -- Study design -- Interval estimation -- Test of hypotheses -- Nonparametric tests -- Analysis of categorical data -- Analysis of survival data -- Analysis of variance -- Linear regression -- Logistic and proportional hazards regression -- Analysis of survey data
    Notes: 1st ed. has title: Introduction to biostatistics.
    Pages: xvii, 502 p. : ill.
    Edition: 2nd ed.
    ISBN: 9780123694928
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    QH405:35(2) on loan
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  • 6
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    Cham : Springer International Publishing
    Keywords: Life sciences ; Biochemistry ; Plant Biochemistry ; Proteins ; Cell Biology ; Evolutionary Biology ; Life sciences ; Protein Science ; Evolutionary Biology ; Cell Biology ; Plant Biochemistry ; Animal Biochemistry ; Animal Systematics/Taxonomy/Biogeography ; Springer eBooks
    Description / Table of Contents: A review on the origin of the different codes for translation -- Evolution of ribosomes -- Origins and early evolution of ribosomes -- Evolution of ribosomes and ribosomal proteins -- Evolution of Class II aminoacyl synthetases from the antisense of the genes for Class I -- The translation process during the evolutionary transition from prokaryotes to eukaryotes -- Evolution of translational control: from archaea to eukarya -- Distribution of elongation family GTPases EF1A and EFL in basal alveolate lineages -- Evolution of mRNA structure; the eukaryotic mRNA 5´- and 3´- untranslated regions and their role in translational regulation -- How lower eukaryotes contribute on our understanding of eIF4E-dependent translation -- Evolution of functionally divergent eIF4Es and their roles in the regulation of mRNA recruitment and developmental processes -- Evolution of eIF4E and eIF4G interactions in trypanosomes -- Evolution of the functionally divergent eIF4E family members in deuterostomes -- Evolution of the eIF2alpha kinases and translational control -- Evolution and use of eIF2alpha kinases in the apicomplexans and their role in life history of the parasites Plasmodium falciparum and Toxoplasma gondii -- Evolution of the TOR pathway and translational regulation -- Origin and diversification of eIF4E-binding proteins -- Evolution of the protein synthesis machinery in mitochondria -- Relationship between plant translation initiation factors and RNA viruses: An evolutionary perspective -- Evolution of miRNAs and RNA binding proteins in eukaryotes -- Evolution of silencing foci across eukaryotes -- Evolutionary conservation of the machineries coupling RNA transport with translation in eukaryotes
    Abstract: The €œómicś€ era has given a new perspective to the findings on the origin and evolution of the process of translation. This book provides insight into the evolution of the translation process and machinery from a modern perspective. Written by leading experts in molecular biology, this text looks into the origins and evolution of the protein synthetic machinery
    Pages: IX, 564 p. 92 illus., 69 illus. in color. : online resource.
    ISBN: 9783319394688
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Anaesthesia 59 (2004), S. 0 
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1617-4623
    Keywords: Key words Mitochondria ; Processed pseudogenes ; Human Tom20 gene ; Import receptor ; Retroposons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the identification and characterization of Ψ3Tom20, a novel processed pseudogene of the human Tom20 (hTom20) gene, which is 96.2% similarity with the hTom20 cDNA and is 5′ and 3′ truncated. In addition, we present the complete characterization of Ψ1Tom20 and Ψ2Tom20, the two other recently reported members of this pseudogene family. Comparison of the sequences of Ψ3Tom20 with that of the previously reported Ψ2Tom20 revealed and corrected an error in the previously determined sequence of Ψ2Tom20. A detailed analysis of these three pseudogenes, including their flanking regions, is presented. It suggests they probably arose from mRNAs that were polyadenylated at different sites. Possible mechanisms involved in their integration as retroposons are also discussed.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-6830
    Keywords: pineal gland ; GT1–7 cells ; norepinephrine ; tyrosine hydroxylase ; melatonin ; cAMP ; estradiol ; progesterone ; testosterone ; adrenergic receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Steroid hormones act on neuronal communication through different mechanisms, ranging from transynaptic modulation of neurotransmitter synthesis and release to development and remodeling of synaptic circuitry. Due the wide distribution of putative brain targets for steroid hormones, acute or sustained elevations of their circulating levels may affect, simultaneously, a variety of neuronal elements. In an elementary mode of interaction, steroids are able to modulate both the synthesis and release of a neurotransmitter at a particular synapse, and the response of its target postsynaptic cells. Using two neuroendocrine transducing systems—the rat pineal gland and the GT1–7 cell line—we have examined these interactions and the following findings are discussed in this article. 2. In the rat, pineal melatonin production is partially controlled by gonadal hormones. In females, melatonin synthesis and secretion is reduced during the night of proestrus, apparently as a consequence of elevated estradiol and progesterone levels. In males, circulating testosterone seems to be necessary to maintain the amplitude of the nocturnal melatonin peak. 3. Some gonadal effects on pineal activity are exerted on its noradrenergic input, since changes in circulating steroid hormone levels are able to induce acute modifications of tyrosine hydroxylase activity in pineal sympathetic nerve terminals. 4. Gonadal steroids are also able to regulate the response of pineal cells to adrenergic stimulation, since in vivo treatment of both male and female rats with steroid hormone blockers induces profound modifications in adrenergically-induced accumulation of cyclic AMP (cAMP) in dispersed pinealocytes. 5. Direct exposure of pineal cells from gonadectomized female and male rats to estradiol (E2) or testosterone (T), respectively, potentiates pinealocyte response to adrenergic activation. In addition, short-term (15 min) exposure to either progesterone (Pg) or progesterone coupled to bovine serum albumin (P-3-BSA) suppresses the E2-dependent potentiation of adrenergic response in female rat pinealocytes. 6. Exposure of GT1–7 cells to E2 completely blocked the norepinephrine (NE)-induced elevation of cAMP content. In E2-treated GT1–7 cells, additional exposure (15 min) to either Pg or P-3-BSA abolished E2-dependent inhibition of NE responsiveness. In addition, P-3-BSA alone increased basal cAMP levels in GT1–7 cells, regardless previous exposure to E2. 7. In conclusion, there are evidences, both from the current literature and from the present results, supporting the view that in some neuroendocrine systems gonadal hormones modulate neurotransmission by acting, simultaneously, at pre- and postsynaptic sites. The models presented here constitute appropriate examples of this transynaptic mode of steroid action and, therefore, may offer a useful approach to investigate steroid hormone actions on the brain.
    Type of Medium: Electronic Resource
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  • 10
    Keywords: CELLS ; RISK ; INFECTION ; LESIONS ; CERVICAL-CANCER ; intraepithelial neoplasia ; METAANALYSIS ; HIV ; VULVA ; GENOTYPE ATTRIBUTION
    Abstract: Knowledge about human papillomaviruses (HPV) types involved in anal cancers in some world regions is scanty. Here, we describe the HPV DNA prevalence and type distribution in a series of invasive anal cancers and anal intraepithelial neoplasias (AIN) grades 2/3 from 24 countries. We analyzed 43 AIN 2/3 cases and 496 anal cancers diagnosed from 1986 to 2011. After histopathological evaluation of formalin-fixed paraffin-embedded samples, HPV DNA detection and genotyping was performed using SPF-10/DEIA/LiPA25 system (version 1). A subset of 116 cancers was further tested for p16(INK4a) expression, a cellular surrogate marker for HPV-associated transformation. Prevalence ratios were estimated using multivariate Poisson regression with robust variance in the anal cancer data set. HPV DNA was detected in 88.3% of anal cancers (95% confidence interval [CI]: 85.1-91.0%) and in 95.3% of AIN 2/3 (95% CI: 84.2-99.4%). Among cancers, the highest prevalence was observed in warty-basaloid subtype of squamous cell carcinomas, in younger patients and in North American geographical region. There were no statistically significant differences in prevalence by gender. HPV16 was the most frequent HPV type detected in both cancers (80.7%) and AIN 2/3 lesions (75.4%). HPV18 was the second most common type in invasive cancers (3.6%). p16(INK4a) overexpression was found in 95% of HPV DNA-positive anal cancers. In view of the results of HPV DNA and high proportion of p16(INK4a) overexpression, infection by HPV is most likely to be a necessary cause for anal cancers in both men and women. The large contribution of HPV16 reinforces the potential impact of HPV vaccines in the prevention of these lesions.
    Type of Publication: Journal article published
    PubMed ID: 24817381
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