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  • 1
    ISSN: 1432-0584
    Keywords: Granulopoese ; Chalon ; Koloniebildung ; Granulopoiesis ; Chalone ; Colony formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Granulocytic Chalone (GCh) has been highly purified from culture medium conditioned by human peripheral leukocytes. Purification was performed by column chromatography on Sephadex G-25 and G-10, rechromatography on G-10, preparative paper chromatography and thin-layer-chromatography. The inhibitory activity and specificity of GCh was monitored by agar colony formation and3H-thymidine incorporation into bone marrow and thymic cells. The gelchromatographic behavior of GCh is discussed in detail with regard to its molecular weight. The results obtained indicate that GCh may be a small (MW 500–600), acidic and highly polar peptide containing aspartic and glutamic acid among others. The N-terminal aminogroup seems to be blocked.
    Notes: Zusammenfassung Ein Peptid, welches sich wie ein Granulozytenchalon verhält, wurde aus durch normale menschliche Leukozyten konditioniertem Medium gewonnen. Die Reinigung erfolgte durch Säulenchromatographie an Sephadex G-25, G-10, Rechromatographie an G-10, Ionenaustauschchromatographie an Dowex 50, sowie durch präparative Papier- und Dünnschichtchromatographie. Die Hemmwirkung und Spezifität wurden mittels der Koloniebildung in Agar sowie durch3H-Thymidineinbau in Knochenmarks- und Thymuszellen nachgewiesen. Das gelchromatographische Verhalten von GCH wird im Hinblick auf das Molgewicht diskutiert. Demnach scheint es sich um ein saures Peptid vom Molgewicht 500–600 zu handeln, welches neben anderen Aminosäuren Asparaginsäure und Glutaminsäure enthält. Die N-terminale Aminogruppe scheint blockiert zu sein.
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  • 2
    ISSN: 1432-0584
    Keywords: Multiple myeloma ; Combination chemotherapy ; Response rate ; Survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 37 consecutive, previously untreated patients with advanced multiple myeloma (16 patients Stage II, 21 patients Stage III) were treated with a five drug regimen consisting of carmustine, melphalan, vincristine, cyclophosphamide and prednisolone (M-2-protocol) in a prospective manner. Remission was achieved in 24 patients (65%). The median time to remission was 10 weeks, the median duration of remission 15,3 months. Median survival time from the onset of treatment was 24 months for all patients. Responding patients have a projected 65% three year survival. Median survival in non-responders was 10 months. 8 patients died during the first year of treatment. These results do not confirm the favourable results with this drug combination obtained in a previous trial. The discrepancy may be explained by a higher proportion of poor risk patients in the present study.
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  • 3
    ISSN: 1432-0584
    Keywords: Granulocyte transfusion ; Graft-versus-Host reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fatal Graft-versus-Host reactions (GVHR) were observed in a 5-year-old boy with Hodgkin's disease after application of non irradiated granulocytes derived from his mother and in a 59 year old female suffering from glioblastoma following transfusion of buffy coat cells from fresh blood units. Clinical signs as well as histopathological changes found in skin biopsies and subsequently at autopsy, were typical for GVHR. In one of the cases the sex difference between donor and recipient provided evidence for engraftment of donor lymphocytes.
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  • 4
    ISSN: 1432-0584
    Keywords: Allogeneic bone marrow transplantation (BMT) ; Cytogenetic studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Chromosome studies were performed in 24 patients who underwent allogeneic bone marrow transplantation (BMT) for severe aplastic anaemia (8), chronic myeloid leukemia (5 in chronic, 2 in accelerated phase and 1 in lymphoid blast crisis), acute myeloid leukemia (6), acute lymphoblastic leukemia in relapse (1) and Hodgkin's disease (1). Donor-cell type engraftment was demonstrated in 21 patients: in all 17 sex-mismatched transplants and — as demonstrated by reconstitution with Ph-negative cell populations — in 4 CML patients with a sex-matched donor. Recipient-type mitoses were seen in the bone marrow of 5 cases (1 SAA, 3 CML, 1 AML) after transplantation. They were only observed on one occasion in patients with SAA (4 of 25 on day 33) and AML (44 of 50 on day 14). Despite the continued demonstration of some Ph-positive mitoses in 3 patients with CML up to day 28, 323 and 451 after BMT, respectively, all surviving CML patients are still in complete haematological and clinical remission. So far the significance of these cytogenetically abnormal persisting host cells remains unknown.
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  • 5
    ISSN: 1432-0584
    Keywords: SAA ; ATG/MP ; T cell subpopulations ; HLA-DR antigen expression suppressor/inducer subset
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In patients with severe aplastic anemia (SAA), lymphocyte subpopulations were examined for the presence of HLA-DR and 2H4 (suppressor/inducer subset) antigen-expressing cells by flow cytometric analysis. Investigations were performed on peripheral blood lymphocytes before and after therapy with antithymocyte globulin (ATG) and methylprednisolone (MP), as well as on bonemarrow lymphocytes before therapy. Before treatment, only the absolute numbers of CD4+ T cells and the CD4+HLA−DR+/CD8+HLA-DR+ activated T cell ratio were significantly decreased (p〈0.01 and p〈0.001, respectively). Following successful ATG/MP treatment, a decrease in the CD4+/CD8+T cell ratio was found. Regarding the suppressor/inducer subset, only absolute numbers of CD4+/2H4+ cells were somewhat higher in treated patients; the percentages were the same in all groups of patients. Studies performed on bonemarrow lymphocytes showed significantly decreased percentages of CD4+ and CD8+ T lymphocytes, which also express HLA-DR antigen. No significant changes in the distribution of activated T cells following ATG/MP therapy were found, suggesting that these cells play no major role in the pathogenesis of the disease.
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  • 6
    ISSN: 1432-0584
    Keywords: Autoimmune thrombocytopenia ; Platelet glycoproteins ; Marrow transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We report the case of a patient who developed autoimmune thrombocytopenia after allogeneic bone marrow transplantation from her HLA-identical sister. An IgG autoantibody was detected that bound to the platelet glycoprotein IIb/IIIa and to the HLA class I proteins. Immunoprecipitation studies with radiolabeled platelets revealed additional antibody binding sites on proteins of 214 kDa molecular weight under nonreduced conditions and 65 and 56 kDa molecular weight under reduced conditions.
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  • 7
    ISSN: 1432-0584
    Keywords: Acute myelogenous leukemia ; Allogeneic marrow transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Between February 1982 and April 1995, 62 patients (37 male, 25 female) with acute myelogenous leukemia (AML) with a median age of 32 years (19–51 years) received allogeneic marrow grafts from an HLA-identical sibling (n=60) or an HLA-mismatched family member (n=2). At the time of transplant, 35 patients were in first complete remission (CR), five in second CR, eight were primary refractory, eight were in untreated relapse and six in refractory relapse. The FAB subtypes were as follows: M1 (n=17), M2 (n=13), M3 (n=6), M4 (n=19), M5 (n=6), M6 (n=1). For conditioning most patients were given total body irradiation combined with cyclophosphamide (CY,n=50) or CY and busulfan (n=9). For graft-versus-host disease prophylaxis patients received cyclosporin A (CSA) and methotrexate (MTX) (n=32), MTX alone (n=12), CSA and methylprednisone (n=5), or CSA alone (n=13). As of April 1995, probability of leukemia-free survival projected at 10 years after BMT was 60% for patients transplanted in first CR compared with 10% for patients transplanted beyond first CR. Transplant-related mortality was 11% after BMT in first CR and 39% after BMT beyond first CR. Probability of relapse projected at 10 years after BMT is 32% for patients who received transplants in first CR and 81% for patients who received transplants beyond first CR. Thus, high-dose chemo/radiotherapy followed by allogeneic marrow infusion has a high curative potential for patients with AML who receive transplants in first CR and offers the chance of long-term disease-free survival for some patients with advanced disease.
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  • 8
    ISSN: 1432-0584
    Keywords: Key words Related and unrelated donor BMT ; CML ; Long-term follow-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Between January 1983 and December 1997, 88 patients (36 female, 52 male, median age 37 years, range 19–57) with chronic myelogenous leukemia (CML) underwent allogeneic bone marrow transplantation (BMT) at the University Hospital of Vienna. Sixty patients were in chronic phase, 18 in accelerated phase, and ten in blast crisis. Marrow donors were HLA-identical siblings for 64 patients (BM 58, PBSC 6), 2-antigen-mismatched related donors (RD) for two, HLA-identical unrelated donors (URD) for 17, and 1-antigen-mismatched URD for five. The median time from diagnosis to BMT was 22 months (range 2–91), and 63 patients had received prior interferon (IFN)-alpha therapy, 46 (73%) for more than 6 months. Conditioning therapy consisted of cyclophosphamide (CY) and total body irradiation (TBI) in 71 patients and CY and busulfan (BU) in 16. One patient received etoposide and TBI. For graft-versus-host disease (GVHD) prophylaxis methotrexate (MTX) was given to 12 patients, MTX and cyclosporin A (CSA) to 67, CSA alone to four, and CSA and methylprednisolone to five. Durable engraftment was documented in 80 of 82 patients (98%). As of December 31, 1997, 52 patients (59%) were alive, 38 (58%) after sibling transplantation with a median observation time of 73 months and 14 (64%) after URD transplantation with a median observation time of 12 months. Probability of overall survival is 59%, for patients undergoing transplantation in chronic phase and 44% for patients undergoing transplantation in advanced stage CML. Probability of disease-free survival (DFS) after sibling and URD BMT is 55% and 59%, respectively. Ten patients (12%) experienced relapse of CML. Transplant-related mortality was 32% both after RD and after URD transplantation. Acute GVHD occurred in 53 of 80 evaluable patients (66%), consisting of grade III or IV in 14 patients (18%). Chronic GVHD developed in 40 of 63 eligible patients (63%), including extensive disease in 26 patients (41%). Thus, sibling and URD BMT offer high cure rates with acceptable toxicity to patients with CML.
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  • 9
    ISSN: 1432-0584
    Keywords: Koloniestimulierende Aktivität ; Menschliches Lungen-gewebe ; Alveolar Makrophages ; Colony stimulating activity ; Human lung tissue ; Alveolar macrophages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Medium conditioned by human lung tissue was found to contain colony stimulating activity (CSA). This material was tested against mouse and human bone marrow as target system. Colony forming units (CFUc)from both species responded and gave rise to clonal growth in agar cultures. This colony formation was dose dependent and the relationship was a sigmoid one. Experiments to determine the molecular weight of human lung derived colony stimulating Factors brought evidence for four active molecular weight fractions with approximately 79000, 40000, 23000 and 2000 daltons. The 23000 dalton fraction activated human cells only, whereas the other fractions were active on both human and mouse bone marrow cells.
    Notes: Zusammenfassung Medium, welches durch menschliches Lungengewebe konditioniert wird, enthält kolonienstimulierende Aktivität (= Colony Stimulating Activity, CSA). Diese Präparation wurde an Menschen- und Mäuseknochenmark getestet. Kolonienbildende Einheiten von beiden Species wurden in Agarkulturen zu klonalem Wachstum aktiviert. Es zeigte sich eine sigmoide Dosisabhängigkeit des Wachtums der CFUc von der Konzentration an lungenkonditioniertem Medium. Die Molekulargewichtsbestimmung der kolonienstimulierenden Faktoren in Lungenmedium ergab vier Molekulargewichtsfraktionen, nämlich mit annähernd 79000,40000,23000und 2000 Daltons. Die 23 000 Dalton-Fraktion aktivierte ausschließlich menschliche Zellen, die übrigen Fraktionen aktivierten Menschen- und Mäuseknochenmarkzellen.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 47 (1983), S. 361-370 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
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