Description / Table of Contents:
Nitrogenases -- Bacteriochlorophyll and Coenzyme F430 -- Carbon Monoxide Dehydrogenases -- Molybdenum-Containing Enzymes -- Hydrogenases -- Genomic Manipulations of the Diazotroph Azotobacter vinelandii -- Purification of Nitrogenase Proteins -- Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a,c-Diamide Reductase -- Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor -- Crystallization of Nitrogenase Proteins -- X-Ray Crystallography of Carbon Monoxide Dehydrogenases -- X-Ray Absorption Spectroscopy of Metalloproteins -- Electron Paramagnetic Resonance Spectroscopy of Metalloproteins -- Magnetic Circular Dichroism Spectroscopy of Metalloproteins -- Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site -- Computational Methods for Modeling Metalloproteins
This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems
X, 275 p. 62 illus., 40 illus. in color. : online resource.