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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    ISSN: 0887-6177
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine , Psychology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1238
    Keywords: Hemodialysis ; Hyperkalemia ; Cardiopulmonary resuscitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective To investigate the efficacy of hemodialysis during cardiopulmonary resuscitation as an effective adjunct to the treatment of severe hyperkalemia. Design A prospective study. Setting In hospital dialysis units and intensive care units. Patients Renal failure patients who developed hyperkalemia induced cardiac arrest and failed to recover from conventional cardiopulmonary resuscitation (CPR) were included. Three patients entered into this study: 2 patients with chronic renal failure maintained on regular hemodialysis and one with acute renal failure who suffered from severe hyperkalemia. Interventions All three patients developed asystolic cardiac arrest with unrecordable blood pressure due to severe hyperkalemia. Aggressive CPR together with intravenous epinephrine, sodium bicarbonate and calcium chloride were instituted. External cardiac massage with cardiac defibrillation was unable to restore spontaneous heart action. After lack or response to intensive resuscitation, hemodialysis was performed concomitant with CPR to eliminate the potassium load. Measurements and results Sinus rhythm and blood pressure were restored in all 3 patients but one of them eventually succumbed to her underlying disease. Conclusion Hemodialysis during CPR is probably an effective adjunct to the treatment of severe hyperkalemia in patients with severe hemodynamic compromise and asystolic cardiac arrest.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1084
    Keywords: Trauma ; Epidermal cyst ; Computed tomography ; Magnetic resonance imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An unusual case of rapid-growing posttraumatic gluteal epidermal cyst with ischiorectal and presacral extension is reported. Pertinent clinical history and magnetic resonance imaging allowed accurate preoperative diagnosis. Cautious complate excision was important to prevent recurrence. To our knowledge, posttraumatic gluteal epidermal cyst with this unusual clinical presentation has not been described previously.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The chromosome constitutions of 15 forms (2 acrodonts and 13 pleurodonts) of amphisbaenians belonging to two families and eight genera were studied. The diploid chromosome numbers varied from 30 to 44 with the number of chromosome arms (FN) ranging from 42 to 60. The karyotypes of 12 forms all contained 6 pairs of macrochromosomes with similar morphology and 9 to 12 pairs of microchromosomes. The remaining three species had a higher diploid number (40–44) and a wide variation in chromosome morphology. Karyotype variations were found in the same family, even in the same genus. The lengths of the 6 pairs of macrochromosomes in 5 of the 12 forms were measured and compared. This disclosed similarities in the mean lengths, relative lengths, and arm ratios of those forms. — Chromosome patterns found in the present study support, in general, the classification of amphisbaenians derived from analysis of morphological characters.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The diploid chromosome number of two species of sidenecked turtles (Podocnemis unifilis and P. expansa) was found to be 28. Under normal culture conditions, half of the chromosomes of P. unifilis consistently show one or two clear secondary constrictions. In P. expansa, the incidence of cells with chromosomes bearing secondary constrictions and the number of such chromosomes per cell are less. Cells of two P. unifilis cell lines maintained a normal diploid karyotype for two years following their initiation. Then one cell line shifted to a hypodiploid mode of 27 and half of the population of the second line became pseudodiploid, the other half remaining diploid. A single six-month-old cell line from P. expansa has maintained a normal diploid mode through 10 passages.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0020-0891
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1434-4726
    Keywords: Fibronectin ; Basal cell ; Cholesteatoma ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fibronectin was localized in human cholesteatoma tissues by immunohistochemical methods. The avidin-biotin-peroxidase complex staining method was used with specific fibronectin antibody. Fibronectin appeared to be localized in the matrix of the cholesteatoma studied, particularly on the surface of the cell membranes and the nuclei of the basal cells and in connective tissue. Fibronectin was not seen in the granular layer or in the keratin area. Fibronectin was found on the surface of granulation tissue, mononuclear cells, fibroblasts and endothelial cells of blood vessels. These findings were confirmed by the immunofluorescent staining method. Our previous study showed that fibronectin induced a migration of keratinocytes, macrophages and fibroblasts demonstrated by the Boyden's chamber chemotaxis assay. Macrophages and fibroblasts were shown to produce collagenase, a bone resorption factor, in cholesteatomatous tissue. The present study showed the presence of fibronectin in the matrix of cholesteatoma and granulation tissue, suggesting that fibronectin might play an important role in the clinical development and invasive behavior of cholesteatoma.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1434-4726
    Keywords: Cholesteatoma ; Intercellular adhesion molecule ; Interleukin-1 ; Tumor necrosis factor-alpha
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intercellular adhesion molecule-1 (ICAM-1) may have a role in acquired cholesteatoma, which is usually associated with an inflammatory reaction occurring in the middle ear cavity. The presence of ICAM-1 in human cholesteatomas was demonstrated by an immunoblotting assay using a specific monoclonal anti-ICAM-1 antibody after protein extraction. Distribution of ICAM-1 in the cholesteatoma tissues was then studied by avidin-biotin-peroxidase complex staining. ICAM-1 appeared to be localized on keratinocytes in all layers of the epithelium and on Langerhans cells in both the epithelium and granulation tissue of cholesteatoma. ICAM-1 was not found in the epidermis of normal external ear canal skin, normal tympanic membrane or normal facial skin, but significant staining was seen on keratinocytes of hair follicles and glands in the facial skin. The present study is the first to demonstrate ICAM-1 in cholesteatoma and suggests that it may have an important role in the clinical development of cholesteatoma, including migration, adhesion and proliferation of lymphocytes, Langerhans cells and keratinocytes.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1434-4726
    Keywords: Cholesteatoma ; Ras protein ; Signal transduction ; Growth factor receptors ; Transcription factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Immunolocalization was used to investigate the distribution of ras protein in both congenital and acquired cholesteatoma tissues. A pathological feature of middle ear cholesteatoma included accumulation of keratin debris due to active proliferation and differentiation of keratinocytes. Ras protein localized to the inner plasma membrane and cytoplasm of keratinocytes in all of the epithelial layers in both congenital and acquired cholesteatoma specimens. Keratinocytes in the basal layer were stained more intensely than their differentiated counterparts. Findings also demonstrated that receptors for epidermal growth factor, fibroblast growth factor, and plateletderived growth factor were present in the plasma membrane of keratinocytes. C-jun, a transcription factor was found in the nuclei of keratinocytes of the cholesteatoma epithelium. These findings indicate that ras protein plays a pivotal role in a signal transduction cascade between growth factor receptors in the plasma membrane and the transcription factor, c-jun, in the nuclei of keratinocytes during the growth of cholesteatoma.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1434-4726
    Keywords: Cholesteatoma ; Terminal differentiation ; Transglutaminase ; Cell envelope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The accumulation of desquamated keratinizing squamous epithelial cells appears to be a crucial factor in the pathogenesis of middle ear cholesteatomas. The accumulation of keratin debris is due to the proliferation and the terminal differentiation of basal keratinocytes. Since cholesteatomas are usually associated with inflammatory reactions in the middle ear cavity, we examined the effects of a granulation tissue conditioned medium on the terminal differentiation of basal keratinocytes in vitro. This conditioned medium stimulated the terminal differentiation of basal keratinocytes by showing: (a) increased incorporation of 3H-leucine into cell envelopes; (b) an increased number of SDS-insoluble cell envelopes; and (c) increased transglutaminase activity (as a marker for terminal cellular differentiation). Our present studies further suggest that inflammatory granulation tissue plays an important role in the clinical growth and development of the cholesteatoma.
    Type of Medium: Electronic Resource
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