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  • 1
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    German Medical Science; Düsseldorf, Köln
    In:  67. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 89. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie und 44. Tagung des Berufsverbandes der Fachärzte für Orthopädie; 20031111-20031116; Berlin; DOC03dguD1-4 /20031111/
    Publication Date: 2003-11-11
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 2
    Keywords: GENES ; SACCHAROMYCES-CEREVISIAE ; DISRUPTION ; oligonucleotides ; TRANSFORMATION ; SITE-DIRECTED MUTAGENESIS ; CASSETTES ; PERVASIVE TRANSCRIPTION ; MODULES ; VERSATILE
    Abstract: Here, we report on a novel PCR targeting-based strategy called 'PCR duplication' that enables targeted duplications of genomic regions in the yeast genome using a simple PCR-based approach. To demonstrate its application we first duplicated the promoter of the FAR1 gene in yeast and simultaneously inserted a GFP downstream of it. This created a reporter for promoter activity while leaving the FAR1 gene fully intact. In another experiment, we used PCR duplication to increase the dosage of a gene in a discrete manner, from 1x to 2x. Using TUB4, the gene encoding for the yeast gamma-tubulin, we validated that this led to corresponding increases in the levels of mRNA and protein. PCR duplication is an easy one-step procedure that can be adapted in different ways to permit rapid, disturbance-free investigation of various genomic regulatory elements without the need for ex vivo cloning.
    Type of Publication: Journal article published
    PubMed ID: 25493941
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  • 3
    Abstract: Stable unannotated transcripts (SUTs), some of which overlap protein-coding genes in antisense direction, are a class of non-coding RNAs. While case studies have reported important regulatory roles for several of such RNAs, their general impact on protein abundance regulation of the overlapping gene is not known. To test this, we employed seamless gene manipulation to repress antisense SUTs of 162 yeast genes by using a unidirectional transcriptional terminator and a GFP tag. We found that the mere presence of antisense SUTs was not sufficient to influence protein abundance, that observed effects of antisense SUTs correlated with sense transcript start site overlap, and that the effects were generally weak and led to reduced protein levels. Antisense regulated genes showed increased H3K4 di- and trimethylation and had slightly lower than expected noise levels. Our results suggest that the functionality of antisense RNAs has gene and condition-specific components.
    Type of Publication: Journal article published
    PubMed ID: 27292640
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  • 4
    Abstract: Pervasive transcription of genomes generates multiple classes of non-coding RNAs. One of these classes are stable long non-coding RNAs which overlap coding genes in antisense direction (asRNAs). The function of such asRNAs is not fully understood but several cases of antisense-dependent gene expression regulation affecting the overlapping genes have been demonstrated. Using high-throughput yeast genetics and a limited set of four growth conditions we previously reported a regulatory function for approximately 25% of asRNAs, most of which repress the expression of the sense gene. To further explore the roles of asRNAs we tested more conditions and identified 15 conditionally antisense-regulated genes, 6 of which exhibited antisense-dependent enhancement of gene expression. We focused on the sporulation-specific gene SPS100, which becomes upregulated upon entry into starvation or sporulation as a function of the antisense transcript SUT169. We demonstrate that the antisense effect is mediated by its 3' intergenic region (3'-IGR) and that this regulation can be transferred to other genes. Genetic analysis revealed that SUT169 functions by changing the relative expression of SPS100 mRNA isoforms from a short and unstable transcript to a long and stable species. These results suggest a novel mechanism of antisense-dependent gene regulation via mRNA isoform switching.
    Type of Publication: Journal article published
    PubMed ID: 28977638
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  • 5
    Abstract: Most eukaryotic proteins are N-terminally acetylated. This modification can be recognized as a signal for selective protein degradation (degron) by the N-end rule pathways. However, the prevalence and specificity of such degrons in the proteome are unclear. Here, by systematically examining how protein turnover is affected by N-terminal sequences, we perform a comprehensive survey of degrons in the yeast N-terminome. We find that approximately 26% of nascent protein N termini encode cryptic degrons. These degrons exhibit high hydrophobicity and are frequently recognized by the E3 ubiquitin ligase Doa10, suggesting a role in protein quality control. In contrast, N-terminal acetylation rarely functions as a degron. Surprisingly, we identify two pathways where N-terminal acetylation has the opposite function and blocks protein degradation through the E3 ubiquitin ligase Ubr1. Our analysis highlights the complexity of N-terminal degrons and argues that hydrophobicity, not N-terminal acetylation, is the predominant feature of N-terminal degrons in nascent proteins.
    Type of Publication: Journal article published
    PubMed ID: 29727619
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  • 6
    facet.materialart.
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2016); 20161025-20161028; Berlin; DOCPO14-636 /20161010/
    Publication Date: 2016-10-10
    Keywords: Aseptische Endoprothesenlockerung ; Periprothetisches Gewebe ; Molekulare Komponenten ; Knochenstoffwechsel ; ddc: 610
    Language: German
    Type: conferenceObject
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  • 7
  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 70 (1999), S. 627-634 
    ISSN: 1433-0385
    Keywords: Key words: Ileostomy ; Coloproctectomy ; “High-output” syndrome ; Short-bowel syndrome ; metabolism. ; Schlüsselwörter: Stomaphysiologie ; Coloproktektomie ; „High-output-Syndrom“ ; Kurzdarmsyndrom.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Die Anlage eines Kunstafters kann neben der psychologischen Belastung auch metabolische Konsequenzen haben. Während Colostomata nur geringe physiologische Probleme bedingen, kann eine Ileostomie eine echte Risikosituation darstellen. Wasser- und Elektrolytbalance wie auch die Versorgung des Körpers mit Nährstoffen, Vitaminen und Spurenelementen sind essentielle Leistungen des Dünndarms. Unter besonderen Bedingungen wie „High-output-Syndrom“ oder Kurzdarmsyndrom sind z. T. schwerwiegende Folgen, wie z. B. Niereninsuffizienz, zu beobachten. Die Grundlagen therapeutischer Eingriffsmöglichkeiten wie enterale und parenterale Substitution werden dargestellt.
    Notes: Summary. In addition to psychological stress, stomas may lead to metabolic consequences. Colostomies normally cause only minor physiological problems; however, patients with ileostomies are at risk for severe metabolic disturbances. The small intestine essentially manages the balance of water and electrolytes and the absorption of nutrition. In special circumstances such as “high output” or short-bowel syndrome, dangerous consequences like kidney disorders may arise. Basic therapeutic strategies such as enteral or parenteral substitution are discussed.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1433-0385
    Keywords: Key words: Compartmental syndrome ; Crush syndrome ; Acute renal failure ; Dialysis ; Drug intoxication. ; Schlüsselwörter: Compartmentsyndrom ; Crush-Syndrom ; akutes Nierenversagen ; Nierenersatztherapie ; Intoxikation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Das akute Compartmentsyndrom (CS) ist ein chirurgischer Notfall. Unterschiedliche Funktionsstörungen mit unphysiologischer Druckerhöhung innerhalb eines anatomisch vorgegebenen Fascienraums führen zum Stillstand der Zirkulation. Ursachen sind Traumata, Verbrennungen, Gefäßverletzungen, ungewohnte körperliche Belastung, Einblutung bei Gerinnungsstörung oder das nephrotische Syndrom mit sekundärer Compartmentschwellung. Folgen einer verzögerten Dekompression sind Funktionsausfälle und Kontrakturen. Wir berichten über die Ausbildung eines beidseitigen Unterschenkel-Compartmentsyndroms nach Cannabis- und Rohypnolabusus. Der Patient war nach Drogenkonsum im Schneidersitz eingeschlafen und mit Zeichen des CS nach mehreren Stunden mit Spontanschmerz, massiver Schwellung und Unterschenkelparese wieder aufgewacht. Über eine beidseitige mediane und laterale Längsincision erfolgte die Compartmentfasciotomie aller 4 Unterschenkellogen. Unmittelbar nach Dekompression entwickelte der Patient eine Crush-Symptomatik mit hoher Kreatininkinaseaktivität (CPK, 140 501 U/l) und akutem Nierenversagen (ANV) bei Rhabdomyolyse. Während zweiwöchiger Nierenersatztherapie erholte sich die exokrine Nierenfunktion bei einem aktuellen Serumkreatininspiegel von 0,7 mg/dl vollständig. Nach Akuttherapie und anschließender Rehabilitation zeigt der Patient weder nervale noch vasculäre Schäden.
    Notes: Summary. Acute compartmental syndrome (CS) is a surgical emergency. Different conditions in which high non-physiological pressure appears within a closed fascial space reduce the necessary blood perfusion. CS is caused by trauma, burns, bleeding in patients with coagulopathies, arterial injuries, nephrotic syndrome or unusual physical exercise with secondary compartmental swelling. When decompression occurs too late, permanent loss of function and limb contracture may result. In the following paper we report on a case of four-compartmental syndrome in the lower legs of a patient with drug intoxication. After cannabis consumption, the patient fell asleep sitting cross-legged. During our first examination several hours later, the signs of compartmental syndrome with spontaneous pain, turgid swelling and paresis were present. Bilateral skin incision technique was used to gain entrance into the four compartments in both lower legs. Immediately after the operation, the patient showed crush syndrome with high serum creatine kinase activity 140.501 U/l and acute renal failure caused by rhabdomyolysis. Within 2 weeks of haemofiltration and dialysis, a full recovery to a normal serum creatinine level of 0,7 mg/dl was achieved. After emergency treatment and rehabilitation, the patient showed neither vascular nor neural defects.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Des désordres fonctionnels du sphincter anal et une irrégularité des selles sont souvent rencontrés chez les patients àyant un prolapsus du rectum. La majorité des patients se plaignent d'incontinence ou de comstipation ou des deux. Une large variété de procédés chirurgicaux ont été avancés pour traiter ce problème que ce soit par voie périnéale ou par voie abdominale. Une meilleure connaissance de l'étiologie des prolapsus et surtout les études radioscopiques démontrant l'invagination du côlon dans le rectum ont amené Frykman et Goldberg á proposer une intervention qui traite en même temps le prolapsus et l'excès de longueur du côlon sigmoïde, contribuant ainsi à prévenir la récidive du prolapsus du côlon. Puisque le côlon sigmoïde joue un rôle important dans la chronique, de tel patients relévent doublement de ce procédé.
    Abstract: Resumen Alteraciones funcionales del esfinter anal y del movimentos intestinal y la defecación son hallazgos frecuentes en pacientes con prolapso rectal. La mayoría de estos pacientes sufre incontinencia y estreñimiento, o ambos. Se ha propuesto una gran variedad de procedimientos quirúrgicos para tratar este problema, tanto por abordaje perineal como por abordaje abdominal. El mayor conocimiento sobre la etiología de la procidencia (prolapso mayor) y, especialmente, los estudios floroscópìcos que demuestran la intususcepción del intestino grueso en el recto, llevó a Frykman y goldberg a proponer una operación que al tiempo que corrige el prolapso rectal reseca abundante colon sigmoide, con lo cual se evita un nuevo prolapso del intestino. Puesto que el sigmoide juega un papel de importancia en el estreñimiento crónico, tales pacientes deberiían recibir beneficio adicional con el procedimiento. El propósito de este estudio prospectivo fue evaluar si esta operación es efectiva y segura, y si podría realmente mejorar la función es del esfínter y del colon.
    Notes: Abstract Constipation and incontinence are frequent complications of rectal prolapse. Surgery should not only aim to correct prolapse but also improve bowel and sphincter function. From 1986–1991 42 patients with procidentia were treated by rectopexy and sigmoid resection. The mean age was 61.1 years. Thirty-nine patients were available for follow-up examination. Mean follow-up was 54 months. Functional data were collected prospectively before the operation and at follow-up and included clinical parameters, a constipation score, an incontinence score, anal manometry [mean resting pressure (MRP), mean maximum pressure (MMP)], proctography [anorectal angle (ARA)] and colonic transit studies [mean transit time (MTT), rectosigmoid transit time (RSTT)]. The postoperative complication rate was 7.1% (n=3), mortality was 0%. No recurrence was seen. Constipation complaints improved from 43.6% to 25.6% (p〈0.001) and incontinence from 66.6% to 23.1% (p〈0.001). MRP increased from 36.5 mmHg to 46.0 mmHg and MMP from 90.5 mmHg to 103.0 mmHg (p〈0.001). ARA changed from 102 to 98 degrees (p〈0.001) and correlated with sphincter tone and continence. MTT decreased from 47.8 to 38.5 hours, segmental transit (RSTT) from 21.1 to 12.7 hours (p〈0.001). Our results indicate that rectopexy with sigmoid resection is a safe and effective procedure for rectal prolapse and improves functional disorders of bowel and sphincter.
    Type of Medium: Electronic Resource
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