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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical Systematics and Ecology 12 (1984), S. 189-192 
    ISSN: 0305-1978
    Keywords: Glycine ; Leguminosae ; biochemical systematics ; coumestrol ; daidzein ; flavonols ; genistin ; hybridization ; soybean ; wild perennial species
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Plant Science Letters 26 (1982), S. 191-197 
    ISSN: 0304-4211
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0305-1978
    Keywords: Glycine spp ; Leguminosae ; Papilionoideae ; glyceollin ; phytoalexins
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2242
    Keywords: Glycine max (L.) Merr. ; Linkage ; Recombination ; Urease activity ; Urease antigen ; Urease mRNA ; Sun ; Eu1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four soybean seed urease nulls (lacking both the activity and antigen of the embryo-specific urease) were intermated and the F1 and F2 seed examined for urease activity. Both generations were without urease activity, and the nulls were therefore considered noncomplementing. In crosses of each null line to cultivars homozygous for the allelic, codominantly inherited urease slow or fast isozyme, the F1 seed expressed the embryo-specific urease isozyme of the urease-expressing parent. A 3 ∶ 1 segregation for presence and absence of urease was observed in progeny from F1 and heterozygous F2 plants. The F2 and F3 from fastXnull combinations revealed that urease-positive seed were all phenotypically urease fast, while the same seed from slowXnull combinations showed a segregation of one seed containing a fast urease, either exclusively or in a heterozygous state with the slow isozyme, for every 69 phenotypic slows. Data pooled from F2 plants which segregate for both the presence (Sun) and absence (Sun) of urease and for the fast (Eu1-b) or slow (Eu1-a) urease allele indicate that the null lesion (Sun) is linked to Eu1 by approximately one map unit. The evidence is consistent with two models: (1) sun is an allele at the embryo-specific urease isozyme locus (Eu1) and that a high degree of exchange (and/or conversion) within the locus results in a 1% recombination frequency between the null trait and urease allozyme; (2) sun is at a distinct locus which is separated by one map unit from the embryo-specific urease isozyme locus (Eu1) upon which it acts in the cis position. Polyadenylated embryo RNA from one of the null lines, PI 229324, exhibited no urease template activity in vitro. Thus, the lack of urease antigen is due to lack of accumulation of translatable urease mRNA. The availability of soybeans lacking seed urease should be extremely useful to breeders as a trait for linkage studies and to geneticists as a transformation marker.
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  • 5
    ISSN: 1432-2242
    Keywords: Glycine spp. ; RFLP ; Taxonomy ; Wild perennial species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total DNA from callus tissue of 28 accessions representing seven wild perennial Glycine species was compared using recombinant genomic probes derived from G. max, the soybean. Using two probes, we show that this molecular approach both confirms and extends the model for the taxonomic relationships between the species derived from morphological and cytogenetic data, and that it provides clear evidence that RFLP analysis of genomic sequences has the potential for revealing the derivation of the member species of the wild perennial Glycine taxon. Although, in this preliminary report, the sample size for each species is small, it is clear that the greatest between-accession variation occurs in G. tabacina (B2B2) and G. clandestine (A1A1), suggesting that these may be the taxa from which further speciation occurred in the subgenus.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2242
    Keywords: Cicer arietinum ; Chickpea ; Pachytene karyotype ; Chromosomes ; Meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A standard pachytene karyotype of chickpea (Cicer arietinum L.) is presented for the first time. Individual pachytene chromosomes were identified and described in detail. An idiogram was prepared on the basis of chromosome length, arm ratio, and distribution of heterochromatin and euchromatin. Chickpea pachytene chromosomes belong to the “differentiated” type with darker staining heterochromatin proximal to and lighter staining euchromatin distal to the centromeres. Chromosomes were numbered from 1 to 8 following a descending order of length. The total length of the chromosome complement at pachytene was 335.33 μ, and chromosome size ranged from 58.05 to 30.53 μ.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2242
    Keywords: Pigeonpea ; Wild Cajanus species Trypsin inhibitors ; Chymotrypsin inhibitors Non-denaturing PAGE ; Activity staining, Environment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variation in the trypsin inhibitors (TIs) and the chymotrypsin inhibitors (CIs) among 69 pigeonpea [Cajanus cajan (L.) Millsp.] strains from a wide geographical distribution and among 17 accessions representing seven wild Cajanus species was studied by electrophoretic banding pattern comparisons and by spectrophotometric activity assays. The TI and CI electrophoretic migration patterns among the pigeonpea strains were highly uniform but varied in the inhibitor band intensities. The migration patterns of the inhibitors in the wild Cajanus species were highly species specific. The mean TI activity of pigeonpea strains (2279 units) was significantly higher than that of the wild Cajanus species (1407 units). However, the mean CI activity in the pigeonpea strains (62 units) was much lower than that in the wild species (162 units). Kenya 2 and ICP 9151 were the lowest and the highest, respectively, in both the TI and CI activities among all the pigeonpea strains used in this study. A highly-significant positive correlation was observed between the TI and CI activities. The Bowman-Birk type inhibitors with both TI and CI activities were identified in all the pigeonpea strains and also in the accessions of all the wild species except C. volubilis (Blanco) Blanco. The C. volubilis accession ICPW 169 was found to be ‘null’ for both CI bands and CI activity. Environment, strain, and environment x strain interaction showed highly-significant effects on both the TI and CI activities. Growing the pigeonpea strains at a different environment from their area of adaptation increased TI and CI activities and also altered the maturity period.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-2242
    Keywords: Key words Soybean ; Glycine max (L) Merr. ; Transformation ; Particle bombardment ; Chromosomal aberrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Some of the transgenic soybean [Glycine max (L.) Merr.] plants produced by bombarding embryogenic suspension cultures with DNA-coated particles exhibit morphological aberrations, including stunted plant growth, leathery dark green leaves and partialto-total seed sterility. In general, cultures from two Asgrow soybean lines (A2242, A2872) that were maintained for 8 months or longer produced primary transformants with reduced fertility. Cytological examination (mitotic pro-metaphase to metaphase chromosomes) of cells of suspension cultures, of roots from germinating somatic embryos, and of plants (R0 and R1) derived from A2242, revealed, besides diploidy (2n=40), various chromosomal aberrations such as deletions, duplications, trisomics and tetraploidy. Diploid transgenic plants with a normal karyotype from A2242 generally exhibited good fertility. No chromosomal abnormalities were observed in A2872-derived plants. However, plants regenerated from relatively old cultures of A2872 (more than 1 year in culture) showed a range of phenotypic abnormalities although they all contained 2n=40 chromosomes. These results indicate that soybean genotypes differ in their susceptibility to chromosomal instability induced by tissue culture. Therefore, chromosome analysis of cell cultures and the plants derived from them can help eliminate chromosomally and genetically abnormal material from gene-transfer experiments.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2242
    Keywords: Glycine max ; Glycine soja ; Interspecific hybrid ; Pachytene karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This study was conducted with the objective of determining the genomic relationship between cultivated soybean (Glycine max) and wild soybean (G. soja) of the subgenus Soja, genus Glycine. Observations on cross-ability rate, hybrid viability, meiotic chromosome pairing, and pollen fertility in F 1 hybrids of G. max × G. soja and reciprocals elucidated that both species hybridized readily and set mature putative hybrid pods, generated vigorous F1 plants, had a majority of sporocytes that showed 18II + 1IV chromosome association at diakinesis and metaphase I, and had a pollen fertility that ranged from 49.2% to 53.3%. A quadrivalent was often associated with the nucleolus, suggesting that one of the chromosomes involved in the interchange is a satellited chromosome. Thus, G. max and G. soja genetic stocks used in this study have been differentiated by a reciprocal translocation. Pachytene analysis of F1 hybrids helped construct chromosome maps based on chromosome length and euchromatin and heterochromatin distribution. Chromosomes were numbered in descending order of 1–20. Pachytene chromosomes in soybean showed heterochromatin distribution on either side of the centromeres. Pachytene analysis revealed small structural differences for chromosomes 6 and 11 which were not detected at diakinesis and metaphase I. This study suggests that G. max and G. soja carry similar genomes and validates the previously assigned genome symbol GG.
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  • 10
    ISSN: 1432-2242
    Keywords: Chemotaxonomy ; Cultivated plants ; Germplasm ; Polyploidy ; Protein profile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seed protein electrophoresis is increasingly being utilized as an additional approach for species identification and as a useful tool for tracing back the evolution of various groups of plants. This paper summarizes the main features of the seed protein profile — stability, uniformity and additive nature. In addition, the significance of this approach for resolving specific taxonomic and evolutionary problems is pointed out.
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