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  • 1
    Keywords: CELLS ; EXPRESSION ; IN-VITRO ; INHIBITOR ; proliferation ; CELL ; CELL-PROLIFERATION ; Germany ; COMMON ; POPULATION ; GENE ; GENE-EXPRESSION ; GENES ; transcription ; ADHESION MOLECULES ; MOLECULES ; PATIENT ; TRANSCRIPTION FACTOR ; DOWN-REGULATION ; MOLECULE ; BONE-MARROW ; TRANSCRIPTION FACTORS ; gene expression ; ASSAY ; affymetrix ; leukemia ; ADHESION ; MIGRATION ; STEM-CELLS ; PHENOTYPE ; PROGENITOR CELLS ; PROTEIN-KINASE-C ; expression profiling ; FIBRONECTIN ; INHIBITORS ; cell proliferation ; ASSAYS ; PHASE ; BCR-ABL ; CML ; BONE ; STEM-CELL ; PROGENITORS ; CXCR4 ; MARROW ; QUIESCENCE ; TRANSCRIPTION-FACTOR ; myeloid ; stem and progenitor cells ; POSITIVE CELLS ; PROPORTION
    Abstract: We found that composition of cell subsets within the CD34+ cell population is markedly altered in chronic phase (CP) chronic myeloid leukemia (CML). Specifically, proportions and absolute cell counts of common myeloid progenitors (CMP) and megakaryocyte-erythrocyte progenitors (MEP) are significantly greater in comparison to normal bone marrow whereas absolute numbers of hematopoietic stem cells (HSC) are equal. To understand the basis for this, we performed gene expression profiling (Affymetrix HU-133A 2.0) of the distinct CD34+ cell subsets from six patients with CP CML and five healthy donors. Euclidean distance analysis revealed a remarkable transcriptional similarity between the CML patients' HSC and normal progenitors, especially CMP. CP CML HSC were transcriptionally more similar to their progeny than normal HSC to theirs, suggesting a more mature phenotype. Hence, the greatest differences between CP CML patients and normal donors were apparent in HSC including downregulation of genes encoding adhesion molecules, transcription factors, regulators of stem-cell fate and inhibitors of cell proliferation in CP CML. Impaired adhesive and migratory capacities were functionally corroborated by fibronectin detachment analysis and transwell assays, respectively. Based on our findings we propose a loss of quiescence of the CML HSC on detachment from the niche leading to expansion of myeloid progenitors. Leukemia (2009) 23, 892-899; doi:10.1038/leu.2008.392; published online 22 January 2009
    Type of Publication: Journal article published
    PubMed ID: 19158832
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  • 2
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A patient with a pure red cell aplasia (Blackfan-Diamond Anemia), and with many congenital abnormalities and growth retardation, has been found to have a chromosome breakage syndrome. In this patient, the frequencies of spontaneous chromosome aberrations and micronuclei in PHA stimulated peripheral blood lymphocytes are elevated when compared to those in normal individuals. The frequency of sister chromatid exchanges is within normal range. The response to mitomycin C (MMC) in the micronucleus test, using lymphocytes, shows a similar increase in the patient's lymphocytes to that in normal individuals, indicating no increased sensitivity to MMC. The frequencies of X-ray induced dicentric chromosomes and micronuclei in the peripheral blood lymphocytes are elevated in the patient. But as the patient clinically does not have any signs of ataxia telangiectasia, this combination of clinical and laboratory findings of this case does not correspond with any of the other known ‘chromosome breakage’ syndromes.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0019-1035
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objectives: Primary objective – To determine the effect of varying brushing forces and brushing times upon the plaque-removing efficacy of a powered toothbrush. Secondary objective – to determine the optimum combination of brushing force and time for plaque removal.Material and Methods: This randomised, single-blind, 16-cell, cross-over trial compared the efficacy of plaque removal of a powered toothbrush (PTB) used with four brushing forces (75, 150, 225 and 300 g) and over four brushing times (30, 60, 120 and 180 s). Twelve volunteers (18–30 years) were recruited and trained to use the Philips/Jordan Sensiflex 2000 PTB, which was modified so that specified forces and times could be recorded. Each subject was asked to abstain from all oral hygiene procedures for 24 hs prior to each brushing event, to allow plaque accumulation. Plaque was recorded using a modified Quigley & Hein index (PI) at six points per tooth before and after each episode of brushing, and the differences in the means (pre- to postbrushing) were compared. Three-way anova was undertaken to compare differences between plaque-removing efficacy for the 16 combinations of force and time. Subjects, brushing time and brushing force were used as fixed effects within the analysis.Results: All 12 subjects participating completed the 16 combinations of force and time over which plaque removal was assessed. Statistically significant differences in PI reductions were found between different brushing times and forces over all tooth surfaces (p〈0.001). The interaction of varying brushing force and brushing time was significant for full mouth (FM) and interproximal (IP) sites (p〈0.03). No statistical significance was demonstrated for smooth surface (SS) sites, nor was it detected for the interaction of subjects with brushing force or subjects with brushing time (p〉0.05).Conclusion: We conclude that brushing time and brushing force have significant effects upon the level of plaque removal by a PTB, and at 120 s brushing time the improvement in plaque removal with forces in excess of 150 g was negligible.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1434-3940
    Keywords: Schlüsselwörter Orbitarekonstruktion ; Resorbierbare Implantate ; Polylaktat ; Key words Orbital reconstruction ; Biodegradable implants ; Polylactide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In a complex animal model in sheep, polydioxanone (PDS®) and polylactic membranes were used for the reconstruction of large orbital-wall defects. In a long-term experiment over 1 year, polylactic implants alone showed the best performance as compared with combinations involving autogenous bone grafts and titanium miniplate fixation. As soon as these polylactic implants are approved for human surgery, they will be used to solve the still challenging problem of anatomical reconstruction of large comminuted fractures of more than one orbital wall.
    Notes: Zusammenfassung In einem experimentellen Modell wurden biodegradierbare Membranen aus Polydioxanon (PDS ® ) resp. Polylaktat zur Rekonstruktion großer Orbitawanddefekte bei Schafen verwendet. Im Langzeitversuch über ein Jahr zeigten diejenigen Rekonstruktionen die besten Resultate, bei welchen ausschließlich biodegradierbare Implantate aus Polylaktat zur Anwendung kamen. Bei gleichzeitiger Verwendung von autologen Knochentransplantaten und Titan-Miniplatten-Osteosynthesen war die anatomische Form der rekonstruierten Orbita weniger zuverlässig gewährleistet. Es darf erwartet werden, dass die klinische Zulassung der Polylaktat-Implantate die chirurgische Wiederherstellung ausgedehnter Trümmerfrakturen mehrerer Orbitawände wesentlich erleichtern wird und zu vorhersehbaren, anatomischen Rekonstruktionen führt.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 202 (1964), S. 203-204 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Patients suffering from various kinds of cancer with metastasis served as volunteers for these tests. Most of them received radiotherapy before or during the test period. This therapy was not administered to the skin being used to determine the skin catalase activity. Known inhibitors of ...
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objectives: To compare 2 prototype powered toothbrush (PTB) heads (A, B) to a marketed head (Sensiflex 2000) for plaque removing efficacy on the Philips/Jordan HX2550 PTB.Materials and Methods: A 2-week, 3-group, single-blind trial recruited 78 volunteers (18–25). Plaque indices (PIs) were recorded at screening and 14 days later at baseline. Subjects were stratified (gender and PI) and then asked to abstain from oral hygiene measures for 48 h. A supervised episode of brushing with the allocated PTB head followed. Subjects then used the PTBs at home for the next 12 days before being asked to abstain from all oral hygiene measures for another 48 h prior to a second supervised brushing episode. Plaque was scored using a new modification of the Quigley & Hein Index (PI) at full mouth (FM), interproximal (IP) and smooth surfaces (SS). PIs were recorded before and after the supervised brushing episodes to enable the means of the within subject differences (pre- to post-brushing) to be compared between groups (ANOVA/t-tests).Results: There were no significant differences in PI between groups at baseline or prior to the supervised brushings (p〉0.05). Highly significant, mean reductions in PIs (approximately 1/2 of one PI unit) were observed for design B compared with the Sensiflex 2000 at FM and IP sites (p〈0.0001) for both supervised brushing episodes. A significant reduction in PI at SS was seen only after the first brushing episode (p=0.0004). For design A, PI reductions were consistently greater than those for the Sensiflex 2000 but differences were only significant at the p〈0.05 level.Conclusion: It is concluded that this model of clinical trial has sufficient power to demonstrate clinical superiority with respect to plaque removal for PTBs and can be used for testing one or more prototype designs of brush heads.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective To evaluate transcervical amnioinfusion for meconium stained amniotic fluid during labout.Design Multicentre randomised controlled trial.Setting Four urban academic hospitals in South Africa. Obstetric surveillance included the use of electronic fetal heart rate monitoring in most cases.Participants Women in labour at term with moderate or thick meconium staining of the amniotic fluid.Interventions Transcervical amnioinfusion of 800 mL saline at 15 mL per minute, followed by a maintenance infusion at 3 mL per minute. The control group received routine care. Blinding of the intervention was not possible.Main outcome measures Caesarean section, meconium aspiration syndrome and perinatal mortality.Results Caesarean section rates were similar (amnioinfusion group 70/167 vs control group 68/159; RR 0.98, 95% CI 0.76–1.26). The incidence of meconium aspiration syndrome was lower than expected on the basis of previous studies (4/162 vs 6/163; RR 0.67, 95% CI 0.19–2.33). There were no perinatal deaths. There were no significant differences between any of the subsidiary outcomes.Conclusions This study concurred with three previous trials which found no effect of amnioinfusion for meconium stained amniotic fluid on caesarean section rate, though the pooled data from all identified trials to date show a significant reduction. The findings with respect to meconium aspiration syndrome were inconclusive in this study alone because of the small number of babies affected, but the point estimate of the relative risk was consistent with the finding of a significant reduction in previous studies and with the Zimbabwe arm (CRAMP 2) of this study. Pooled data clearly support the use of amnioinfusion for meconium stained amniotic fluid to reduce the incidence of meconium aspiration syndrome.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We report a highly polymorphic poly-A sequence in the 5′ flanking region of CYP11B2 that may be used in genetic counselling for prenatal diagnosis or carrier detection in families affected by steroid 11β-hydroxylase genes defects.
    Type of Medium: Electronic Resource
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