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  • 1
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    Totowa, N.J. : Humana Press
    Associated volumes
    Call number: QH506:60/565
    Keywords: Drug Evaluation, Preclinical / methods ; Chemistry, Pharmaceutical
    Pages: xii, 268 p. : ill.
    Edition: 2nd. ed.
    ISBN: 9781603272575
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    QH506:60/565 available
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  • 2
    Keywords: Chemistry ; Biotechnology ; Chemistry ; Biotechnology ; Springer eBooks
    Description / Table of Contents: Design and Implementation of High Throughput Screening Assays -- Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method -- High Throughput Screening Using Mass Spectrometry within Drug Discovery -- Structure-Based Virtual Screening of Commercially Available Compound Libraries -- AlphaScreen-Based Assays: Ultra-High Throughput Screening for Small Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions -- Instrument Quality Control -- Application of Fluorescence Polarization in HTS Assays -- Time Resolved Fluorescence Assays -- Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays -- Screening for Inhibitors of Kinase Autophosphorylation -- A Fluorescence-Based High Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus -- Mycobacterium tuberculosis High Throughput Screening -- Identification of State-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR Based Assay -- A Luciferase Reporter Gene System for High Throughput Screening of γ-Globin Gene Activators -- A High Throughput Flow Cytometry Assay for Identification of Inhibitors of 3’, 5’-Cyclic Adenosine Monophosphate Efflux -- High Throughput Cell Toxicity Assays -- BRETn: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells -- Application of Imaging-Based Assays in Microplate Formats for High Content Screening
    Abstract: This third edition volume expands on the previous editions both by presenting more detailed protocols for the techniques described in the first and second editions of High Throughput Screening: Methods and Protocols and by covering important new procedures. The first chapter of this book provides an overview of important assay development techniques, while the rest of the chapters detail how to develop and execute screens at whatever throughput the user needs. Some chapter examples are: structure-based virtual screening, high throughput screening using mass spectrometry, identification of state-dependent blockers for voltage gated calcium channels, bioluminescence resonance energy transfer platform to monitor protein-protein interactions in live cells, high throughput flow cytometry, and application of imaging-based assays in microplate formats for high content screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, High Throughput Screening: Methods and Protocols, Third Edition, is a valuable resource for anyone who is interested in HTS research
    Pages: XI, 308 p. 101 illus., 71 illus. in color. : online resource.
    Edition: 3rd ed. 2016.
    ISBN: 9781493936731
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  • 3
    Keywords: Life sciences ; Biochemistry ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Validation of Protein Acetylation by Mass Spectrometry -- Application of the CIRAD Mass Spectrometry Approach for Lysine Acetylation Site Discovery -- Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites -- Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations -- Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin -- Identification and Analysis of O-acetylated Sialoglycoproteins -- HPLC-based Quantification of in vitro N-terminal Acetylation -- Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography -- In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation -- Computational Prediction of Lysine Acetylation Proteome-wide -- Generation and Characterization of Pan-specific Anti-acetyllysine Antibody -- Using Functional Proteome Microarrays to Study Protein Lysine Acetylation -- Quantitation of Nucleosome Acetylation and other Histone Post-Translational Modifications Using Microscale NU-ELISA -- Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core -- Production of Amino-terminally Acetylated Recombinant Proteins in E. coli -- Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics -- Non-radioactive in-vitro Assays for Histone Deacetylases -- The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes -- Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators
    Abstract: Thousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of a nascent protein and lysine residues within a protein. In Protein Acetylation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study protein acetylation. These include methods and techniques for identification of protein acetylation, column- and gel electrophoresis-based approaches, computationally prediction,℗ and the biological response to protein acetylation. Written in the highly successful Methods in Molecular Biologý„Ø series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Protein Acetylation: Methods and Protocols seeks to aid scientists in the further study of the technical aspects involved in understanding protein acetylation
    Pages: XI, 266 p. 54 illus., 18 illus. in color. : digital.
    ISBN: 9781627033053
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  • 4
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2018); 20181023-20181026; Berlin; DOCAT22-848 /20181106/
    Publication Date: 2018-11-07
    Keywords: Condylus radialis ; nicht dislozierte Frakturen ; sekundäre Dislokation ; Kinder ; Konservative Behandlung ; Röntgenkontrolle ; ddc: 610
    Language: German
    Type: conferenceObject
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  • 5
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  66. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC); 20150607-20150610; Karlsruhe; DOCDI.10.07 /20150602/
    Publication Date: 2015-06-03
    Keywords: Apomorphine ; Parkinson ; DBS ; ddc: 610
    Language: English
    Type: conferenceObject
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  • 6
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Entscheiden trotz Unsicherheit; 14. Jahrestagung des Deutschen Netzwerks Evidenzbasierte Medizin; 20130315-20130316; Berlin; DOC13ebmP82 /20130311/
    Publication Date: 2013-03-12
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 7
    Abstract: Inhibitors of the receptor tyrosine kinase c-MET are currently used in the clinic to target oncogenic signaling in tumor cells. We found that concomitant c-MET inhibition promoted adoptive T cell transfer and checkpoint immunotherapies in murine cancer models by increasing effector T cell infiltration in tumors. This therapeutic effect was independent of tumor cell-intrinsic c-MET dependence. Mechanistically, c-MET inhibition impaired the reactive mobilization and recruitment of neutrophils into tumors and draining lymph nodes in response to cytotoxic immunotherapies. In the absence of c-MET inhibition, neutrophils recruited to T cell-inflamed microenvironments rapidly acquired immunosuppressive properties, restraining T cell expansion and effector functions. In cancer patients, high serum levels of the c-MET ligand HGF correlated with increasing neutrophil counts and poor responses to checkpoint blockade therapies. Our findings reveal a role for the HGF/c-MET pathway in neutrophil recruitment and function and suggest that c-MET inhibitor co-treatment may improve responses to cancer immunotherapy in settings beyond c-MET-dependent tumors.
    Type of Publication: Journal article published
    PubMed ID: 29045907
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  • 8
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  58. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC); 20070426-20070429; Leipzig; DOCP 023 /20070411/
    Publication Date: 2007-04-04
    Keywords: DBS ; STN ; MER ; Tiefe Hirnstimulation ; STN ; Mikroelektrodenableitungen ; ddc: 610
    Language: English
    Type: conferenceObject
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  • 9
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  65. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC); 20140511-20140514; Dresden; DOCP 003 /20140513/
    Publication Date: 2014-05-14
    Keywords: DBS ; Parkinson ; anesthesia ; ddc: 610
    Language: English
    Type: conferenceObject
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  • 10
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2013); 20131022-20131025; Berlin; DOCPO15-1027 /20131023/
    Publication Date: 2013-10-24
    Keywords: patient tracking ; efficiency ; wlan ; ddc: 610
    Language: English
    Type: conferenceObject
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