Description / Table of Contents:
Parts characterization for tunable protein expression -- Enzyme nicotinamide cofactor specificity reversal guided by automated structural analysis and library design -- Bacterial genome editing strategy for control of transcription and protein stability -- An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization -- Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design -- Extended Metabolic Space Modeling -- Computational Methods to Assess the Production -- Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-gene Systems -- An adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism -- CRISPR-Cas9 toolkit for Actinomycete Genome Editing -- Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast using CasEMBLR -- A modified Gibson assembly method for cloning large DNA fragments with high GC contents -- Coupling Yeast Golden Gate and VEGAS for efficient assembly of the violacein pathway in Saccharomyces cerevisiae -- Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites For Phenotyping Of Microorganisms -- Selection of Highly Expressed Gene Variants in Escherichia coli using Translationally-coupled Antibiotic Selection Markers -- Design, Engineering, and Characterization Of Prokaryotic Ligand-binding Transcriptional Activators as Biosensors in Yeast -- A Capture-SELEX Strategy for Multiplexed Selection of RNA Aptamers against Small Molecules -- High-throughput Microfluidics for the Screening Of Yeast Libraries -- Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution -- Two scale 13C Metabolic Flux Analysis for Metabolic Engineering
This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways℗ for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance.℗ Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering. Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,℗ Synthetic Metabolic Pathways: Methods and Protocols℗ aims to ensure successful results in the further study of this vital field
XII, 354 p. 68 illus., 64 illus. in color. : online resource.