Nerve Growth Factor Antiserum
Quantitative Electron Microscopy
Springer Online Journal Archives 1860-2000
Summary Uninjured and crushed cervical sympathetic trunks from immunosympathectomized and control rats 8 months old were compared by quantitative electron microscopic techniques. It was demonstrated that: 1. The unmyelinated axonal population, reduced by neonatal administration of nerve growth factor-antiserum, remains stable at approximately one-third of normal. 2. Median diameters of unmyelinated axons were significantly smaller for nerves from nerve grwoth factor-antiserum-treated animals than for control nerves. 3. The number of myelinated axons in cervical sympathetic trunks from nerve growth factor-antiserum-treated animals was reduced to approximately 1/20 of normal. 4. By 8 months of age, the configuration of Schwann cell complexes in nerves from immunosympathectomized animals had changed; the elongated processes and collagen pockets which were present at earlier stages were less prominent. 5. 2 weeks after crush injury, axonal sprouting was similar for immunosympathectomized and control cervical sympathetic trunks. Thus, the loss of unmyelinated axons due to neonatal administration of nerve growth factor-antiserum persists without evidence of spontaneous compensatory regeneration. However, the surviving axons are able to sprout in response to a stimulus such as crush injury.
Type of Medium: