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  • 1
    Publication Date: 2018-07-04
    Description: Background/Aim: Bisdemethoxycurcumin (BDMC) exhibits biological activities including anticancer and anti-metastasis in human cancer cell lines, but there is no available information to show whether BDMC suppresses cell migration and invasion of human cervical cancer cells. Materials and Methods: Wound-healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of BDMC on HeLa cells in vitro. Results: BDMC reduced the total viable cell number in a dose-dependent manner. The wound-healing assay show BDMC suppressed the movement of HeLa cells. Furthermore, the trans-well chamber assays showed that BDMC suppressed the cell migration and invasion. Gelatin zymograph assay showed that BDMC did not inhibit matrix metalloproteinase-2 (MMP-2) and -9 activities in vitro. However, western blotting assay showed that BDMC significantly reduced protein levels of growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (Rho A), urokinase-type plasminogen activator (uPA), RAS, MMP-2, and N-cadherin but increased those of phosphor-extracellular-signal related kinase (p-ERK1/2), E-cadherin and nuclear factor-ĸB (NF-ĸB) in HeLa cells. Confocal laser microscopy assay was used to further confirm BDMC increased NF-ĸB when compared to controls. Conclusion: BDMC may have potential as a novel anti-metastasis agent for the treatment of human cervical cancer.
    Print ISSN: 0250-7005
    Electronic ISSN: 1791-7530
    Topics: Medicine
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  • 2
    Publication Date: 2013-07-28
    Description: The essential bacterial protein FtsZ is a guanosine triphosphatase that self-assembles into a structure at the division site termed the "Z ring". During cytokinesis, the Z ring exerts a constrictive force on the membrane by using the chemical energy of guanosine triphosphate hydrolysis. However, the structural basis of this constriction remains unresolved. Here, we present the crystal structure of a guanosine diphosphate-bound Mycobacterium tuberculosis FtsZ protofilament, which exhibits a curved conformational state. The structure reveals a longitudinal interface that is important for function. The protofilament curvature highlights a hydrolysis-dependent conformational switch at the T3 loop that leads to longitudinal bending between subunits, which could generate sufficient force to drive cytokinesis.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816583/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816583/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Li, Ying -- Hsin, Jen -- Zhao, Lingyun -- Cheng, Yiwen -- Shang, Weina -- Huang, Kerwyn Casey -- Wang, Hong-Wei -- Ye, Sheng -- 1F32GM100677-01A1/GM/NIGMS NIH HHS/ -- DP2 OD006466/OD/NIH HHS/ -- DP2OD006466/OD/NIH HHS/ -- F32 GM100677/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2013 Jul 26;341(6144):392-5. doi: 10.1126/science.1239248.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Life Sciences Institute, Zhejiang University, Hangzhou, 310058 Zhejiang, P.R. China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23888039" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Bacterial Proteins/*chemistry/genetics/*metabolism ; Cell Membrane/physiology ; Crystallography, X-Ray ; *Cytokinesis ; Cytoskeletal Proteins/*chemistry/genetics/*metabolism ; Escherichia coli/chemistry ; Guanosine Diphosphate/chemistry/metabolism ; Guanosine Triphosphate/metabolism ; Hydrolysis ; Hydrophobic and Hydrophilic Interactions ; Models, Molecular ; Molecular Dynamics Simulation ; Molecular Sequence Data ; Mycobacterium tuberculosis/*chemistry/physiology ; Point Mutation ; Protein Conformation ; Protein Multimerization ; Protein Subunits/chemistry/metabolism ; Staphylococcus aureus/chemistry
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 2015-05-02
    Description: When Staphylococcus aureus undergoes cytokinesis, it builds a septum, generating two hemispherical daughters whose cell walls are only connected via a narrow peripheral ring. We found that resolution of this ring occurred within milliseconds ("popping"), without detectable changes in cell volume. The likelihood of popping depended on cell-wall stress, and the separating cells split open asymmetrically, leaving the daughters connected by a hinge. An elastostatic model of the wall indicated high circumferential stress in the peripheral ring before popping. Last, we observed small perforations in the peripheral ring that are likely initial points of mechanical failure. Thus, the ultrafast daughter cell separation in S. aureus appears to be driven by accumulation of stress in the peripheral ring and exhibits hallmarks of mechanical crack propagation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhou, Xiaoxue -- Halladin, David K -- Rojas, Enrique R -- Koslover, Elena F -- Lee, Timothy K -- Huang, Kerwyn Casey -- Theriot, Julie A -- 1S10OD01227601/OD/NIH HHS/ -- DP2OD006466/OD/NIH HHS/ -- P50-GM107615/GM/NIGMS NIH HHS/ -- R01 AI036929/AI/NIAID NIH HHS/ -- R01-AI36929/AI/NIAID NIH HHS/ -- R37 AI036929/AI/NIAID NIH HHS/ -- T32 GM007276/GM/NIGMS NIH HHS/ -- T32-GM007276/GM/NIGMS NIH HHS/ -- U54-GM072970/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2015 May 1;348(6234):574-8. doi: 10.1126/science.aaa1511.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, Stanford University, Stanford, CA 94305, USA. Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. ; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. ; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. ; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. theriot@stanford.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25931560" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Wall/physiology/ultrastructure ; *Cytokinesis ; Microscopy, Electron, Scanning ; Microscopy, Video ; Staphylococcus aureus/cytology/*physiology/ultrastructure ; Stress, Mechanical ; Time Factors
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Heteroepitaxial growth of InP on Si with an intermediate GaAs buffer layer by low-pressure organometallic vapor phase epitaxy is reported. Excellent crystallinity of InP epilayers with specular surfaces can be reproducibly obtained. The carrier concentration profile shows that the carrier distribution in the InP layer is very uniform, while an apparent reduction in concentration occurs at the InP/GaAs interface. The 77 K photoluminescence (PL) of the InP layer exhibits a strong near-band-edge emission. No evident shift in PL peak energy for the InP/GaAs/Si sample compared with that for the InP homoepitaxial sample was first observed in this study. These results are superior to those reported previously for the InP/Si heteroepitaxy.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We present an integrated device fabrication sequence for GaAs/AlGaAs resonant tunneling diodes and the results of dc and microwave characterization of the integrated devices. The development of an integrated structure represents a first step toward monolithic or hybrid integration for applications such as oscillators in the 100 GHz–1 THz range. The use of a proton implant in addition to a mesa etch for device isolation allows low parasitic capacitance connections to bond pads, interconnects, or radiating elements. The dc and microwave measurement procedures and an equivalent circuit topology for the integrated device are described. Several features associated with the integrated geometry, including a decrease in peak current density with increasing device diameter, are observed in a study of the current-voltage characteristics of devices with various diameters. Contact resistances are determined from a physically based model and compared with experimental results. Microwave characterization techniques are used to obtain microwave equivalent circuit parameters for the integrated diodes. Device capacitance and conductance are presented as functions of device dimension and bias voltage. Parasitic circuit elements, including series resistance and a capacitance and resistance associated with the proton bombardment used to define the mesa, are also determined from the microwave analysis. The results obtained from microwave impedance measurements are compared with parameters obtained from dc characterization and numerical simulations of comparable device structures. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 6
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 161 (1948), S. 400-401 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] FOR centuries in China the roots (Chang Shan) and the leaves (Shuu Chi) of Dichroa febrifuga, Lour, have been used against malarial fevers. In our first report1 in 1943, it was recorded that a crude extract of this root had been effectively used on clinical cases of tertian ...
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0009-8981
    Keywords: CPC - citrate turbidity test ; MPS paper spot test ; Monodimensional electrophoresis ; Screening test
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 305 (1969), S. 155-166 
    ISSN: 1432-2013
    Keywords: l-Glucose ; Micropuncture and Microperfusion ; Proximal Tubule ; Active Secretion ; Kinetic Study ; l-Glucose ; Mikropunktion und Mikroperfusion ; proximaler Tubulus ; aktive Sekretion ; kinetische Studien
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Studies with the free flow micropuncture technique have shown that the ratio of TF/Pl-glucose to TF/PInulin in proximal tubular fluid, in distal tubular fluid, and in more than half of the final urine samples measured was greater than one, which suggests thatl-glucose was actively secreted. Studies with the microperfusion technique confirmed this finding and showed thatl-glucose was secreted by the proximal tubules. A maximum rate of secretion was reached at a plasma concentration of 4 mM. The tubular secretion ofl-glucose was augmented by the presence of 16.6 mMd-glucose in tubular lumen and inhibited by 10−4 M phlorizin. Kinetic analysis showed that theV max values forl-glucose secretion in the absence and in the presence ofd-glucose are 5.0×10−10 and 6.3×10−10 mol×cm−2×sec−1 respectively which were very close to the value reported for theV max ford-glucose reabsorption. However, theK m forl-glucose secretion was 3.1 mM and was reduced to 1.6 mM whend-glucose was present in the perfusion fluid. TheK m ford-glucose reabsorption has been reported to be 0.6 mM (8). The results of this investigation were interpreted as being consistant with the hypothesis thatl-glucose secretion andd-glucose reabsorption share the same carrier system.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Clinica Chimica Acta 151 (1985), S. 141-146 
    ISSN: 0009-8981
    Keywords: GAG ; Glycosaminoglycan ; MPS ; Mucopolysaccharidosis ; Normal range ; Random urine ; Uronic acid to creatinine (UA/C) ratio
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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