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  • 1
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Synthesis and Conjugation of Small Interfering RiboNucleic Neutral siRNNs.-℗ Liver-Targeted siRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates.-℗ PepFect6 Mediated siRNA Delivery into Organotypic Cultures.-℗ Highly Efficient siRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems.-℗ Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for siRNA Delivery.-℗ Preparation of a Cyclic RGD: Modified Liposomal siRNA Formulation for Use in Active Targeting to Tumor and Tumor Endothelial Cells -- A Multifunctional Envelope-Type Nano Device Containing A pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo -- Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of siRNA.-℗ Preparation of Polyion Complex Micelles Using Block Copolymers for siRNA Delivery.-℗ Delivery of Small Interfering RNAs to Cells via Exosomes -- Dendrimer Nanovectors for siRNA Delivery.-℗ Chitosan Nanoparticles for siRNA Delivery In Vitro -- Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for siRNA Delivery.-℗ siRNA In Vivo Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast.-℗ TLR9 Targeted siRNA Delivery In Vivo -- Aptamer-miRNA Conjugates for Cancer Cells Targeted Delivery -- Method for Confirming Cytoplasmic Delivery of RNA Aptamers -- Hapten-Binding Bispecific Antibodies for Targeted Delivery of siRNA and siRNA-Containing Nanoparticles.-℗ Stable Delivery of CCR5 Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector -- Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors.-℗ Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery.-℗ Efficient Gene Suppression in Dorsal Root Ganglia (DRG) and Spinal Cord Using Adeno-Associated Virus (AAV) Vector Encoding Short-Hairpin RNA.-℗ Synthetic siRNA Delivery: Progress and Prospects
    Abstract: This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed℗ and refined over the years with tried-and-true methods. Written by a team of internationally renowned authors, this book describes, in detail, a variety of successful siRNA delivery methods, including peptide-based nanoparticles, liposomes, exosomes, polymers, aptamers, and viral vehicles. Written in the highly successful Methods in Molecular Biology series format, each proven protocol includes brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically
    Pages: XIII, 313 p. 64 illus., 35 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9781493931125
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  • 2
    Keywords: Human Genetics ; Cell Culture ; Human Genetics ; Cell Culture ; Springer eBooks
    Description / Table of Contents: Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis -- Animal Lymphocyte Metaphase Chromosome Preparation -- Micronuclei Formation Analysis after Ionizing Radiation -- G1 Premature Chromosome Condensation (PCC) Assay -- G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity -- G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology -- Sister Chromatid Exchange as a Genotoxic Stress Marker -- DNA Damage Focus Formation Assay -- Nuclear Foci Assays in Live Cells -- In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes -- PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations -- Modified PNA Telomere and Centromere FISH Protocols -- Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements -- Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH -- Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting
    Abstract: This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory
    Pages: X, 138 p. 18 illus., 11 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9781493994328
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  • 3
    Keywords: Medicine ; Oncology ; Food science ; Post-translational modification of proteins ; Biomedicine ; Cancer Research ; Posttranslational Modification ; Food science ; Springer eBooks
    Description / Table of Contents: Preface. I Lipid peroxidation and small molecule adducts -- Lipid hydroperoxides as a source of singlet molecular oxygen -- The formation of lipid hydroperoxide-derived amide-type lysine adducts on proteins: a review of current knowledge -- Lipid hydroperoxide-derived adduction to amino-phospholipid in biomembrane -- Amide-type adduct of dopamine - Plausible cause of Parkinson diseases -- Determination of HEL (hexanoyl-lysine adduct): a novel biomarker for omega-6 PUFA oxidation -- Hexanoyl-lysine as a deterioration marker for rice during storage -- Cholesterol hydroperoxides and their degradation mechanism. II Pathophysiological consequences -- Amide-adducts in atherosclerosis -- Oxidative Modification of Lipoproteins -- Immunochemical Detection of Lipid Hydroperoxide- and Aldehyde-Modified Proteins in Diseases -- Role of Lipid Peroxide in the Neurodegenerative Disorders -- Lipid Hydroperoxide-Derived Modification of Proteins in Gastrointestinal Tract. III Applications for diagnosis and development of functional food -- Low-cost and easy-to-use "on-chip ELISA" for developing health-promoting foods -- Hexanoyl-lysine as an oxidative-injured marker - application of development of functional food -- Potential role of oxidative protein modification in energy metabolism in exercise -- Suppressive effects of cacao polyphenols on the development of atherosclerosis in apolipoprotein E-deficient mice -- Index
    Abstract: This volume focusses on oxidative modifications of lipid molecules and the successive generation of singlet oxygen. The book also covers the secondary adductions of these reactive species with proteins and aminophospholipids. During lipid peroxidation, the initial event is the formation of lipid hydroperoxide, which is followed by an oxidation event that starts a chain-reaction. The formed lipid hydroperoxide gradually decomposes into harmful aldehydes, which are the advanced end-products of lipid peroxidation. The book consists of three sections: ℗ Part I, entitled Lipid peroxidation and small molecule adducts focusses on the biochemical events that are involved in lipid peroxidation and to the formation of small molecules like singlet molecular oxygen. This part of the book also introduces the hexanoyl-lysine adduct. Part II, entitled Pathophysiological consequences covers a range of damaging physiological consequences of lipid peroxidation, ranging from atherosclerosis to neurodegenerative disorders. Finally, Part III, entitled Applications for diagnosis and development of functional food looks into potential diagnostic uses of lipid peroxidation, the possible beneficial effects that can be achieved and new assays in food safely that have been developed
    Pages: VIII, 202 p. 78 illus., 27 illus. in color. : online resource.
    ISBN: 9789400779204
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  • 4
    Keywords: Biochemistry ; Biochemistry, general ; Biological and Medical Physics, Biophysics ; Springer eBooks
    Description / Table of Contents: Preface -- Chapter 1: The effect of N-glycans on glycoprotein folding and protein dynamics -- Chapter 2: Synthesis of glycosylated metal complexes for probing carbohydrate-carbohydrate interactions -- Chapter 3: Unraveling of lipid raft organization in cell plasma membranes by single-molecule imaging of ganglioside probes -- Chapter 4: MALDI mass spectrometry imaging of N-linked glycans in tissues -- Chapter 5: Modern-omics technologies for isobaric glycan and glycoconjugate structure determination -- Chapter 6: Synchrotron-radiation vacuum-ultraviolet circular-dichroism spectroscopy for characterizing the structure of saccharides -- Chapter 7: Biophysical analyses for probing glycan-protein interactions -- Chapter 8: Structural aspects of ER glycoprotein quality-control system mediated by glucose tagging -- Chapter 9: Structural basis of protein Asn-glycosylation by oligosaccharyltransferases -- Chapter 10: Visualization of functional structure and kinetic dynamics of cellulases -- Chapter 11: Structure and dynamics of immunoglobulin G glycoproteins -- Chapter 12: Biophysical Approaches to Solve the Structures of the Complex Glycan Shield of Chloroviruses -- Chapter 13: Quantifying weak glycan-protein interactions using a Biolayer Interferometry competition assay: Applications to ECL lectin and X-31 influenza hemagglutinin
    Abstract: This book presents state of the art biophysical approaches to issues in glycobiology that have cutting-edge applications. Despite the importance of glycosylation, the complexity, heterogeneity, and flexibility of the glycans have inhibited their study. Each chapter in this book explains very recent significant advances in biophysical approaches through the use of techniques such as NMR spectroscopy, mass spectrometry, single-molecule imaging, X-ray crystallography, high-speed atomic force microscopy, and computational simulation and their integrative application. Concrete examples are provided of the value of these techniques in addressing key problems in the field. In addition, significant functional glycobiological issues are considered. For example, glycolipids can form dynamic clusters on cell membranes and provide platforms for molecules involved in cell recognition and subsequent signal transduction. The detailed delineation of these molecular systems is discussed, revealing their structural complexity and ability to assemble transiently. This timely book will be of value for graduate students and postdocs interested in frontier topics in glycoscience and also for senior bio-researchers in academic and industrial fields
    Pages: XIII, 277 p. 110 illus., 74 illus. in color. : online resource.
    ISBN: 9789811321580
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  • 5
    Keywords: Life sciences ; Plant Ecology ; Evolutionary Biology ; Entomology ; Life sciences ; Evolutionary Biology ; Plant Ecology ; Entomology ; Springer eBooks
    Description / Table of Contents: Preface -- Acknowledgments -- Part I Natural History -- 1. Introduction (M. Kato) -- 2 History and Natural History of Plants and Their Associates (M. Kato) -- 3 Biology of the Obligate Pollination Mutualism (M. Kato and A. Kawakita) -- 4 Diversity of Phyllanthaceae (A. Kawakita and M. Kato) -- 5 Diversity of Pollinator Moths (A. Kawakita and M. Kato) -- 6 Origin of Active Pollination and Mutualism (A. Kawakita and M. Kato) -- Part II Ecology -- 7 Reinforced Specificity of Pollinator Moths (A. Kawakita and M. Kato) -- 8 Species-Specific Floral Scents as Olfactory Cues in Pollinator Moths (T. Okamoto) -- 9 Role of Selective Flower Abortion in the Maintenance of Obligate Pollination Mutualism (R. Goto) -- Part III Evolution -- 10 Cospeciation and Host Shift (A. Kawakita and M. Kato) -- 11 Reversal of Mutualism (A. Kawakita) -- 12 Phyllantheae– Mutualistic Interactions on Oceanic Islands in the Pacific (David Hembry) -- 13 Evolution and Diversity of Obligate Pollination Mutualisms (A. Kawakita and M. Kato) -- Literature Cited -- Index
    Abstract: This book presents a comprehensive overview of our current understanding of mutualism origin, plant́€“pollinator specificity, mutualism stability, and reciprocal diversification. In particular, it focuses on the natural history and evolutionary history of the third example of obligate pollination mutualism, leaffloweŕ€“leafflower moth association, which was discovered in the plant family Phyllanthaceae by the lead editor and then established by the editors and their coworkers as an ideal model system for studies of mutualism and the coevolutionary process. This work brings together the knowledge they have gained through an array of research conducted using different approaches, ranging from taxonomy, phylogenetics, ecology, and evolutionary biology to biogeography. Richly illustrated with numerous original color photographs, the volume consists of 13 chapters and is divided into three main parts: natural history, ecology, and evolution. It begins by showcasing numerous examples of plant€“ánimal interactions and their origins to guide readers in the world of leafflowers and their pollinators. The immense diversity of Phyllanthaceae and pollinator moths is then explored, and in the following 7 chapters mutualism is discussed from a range of ecological and evolutionary points of view. The final chapter presents a review of the evolution and variety of obligate pollination mutualisms. This book offers researchers and students in the field of ecology, botany, evolutionary biology, pollination biology, entomology, and tropical biology fascinating insights into why such a costly pollination system has evolved and why Phyllanthaceae is so diverse despite the inconspicuousness of their flowers
    Pages: XII, 309 p. 125 illus., 87 illus. in color. : online resource.
    ISBN: 9784431565321
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  • 6
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In this study, we examined the symptom scores and tumor necrosis factor-alpha (TNF-α), p55 soluble tumor necrosis factor receptor (sTNFR1), and p75 soluble tumor necrosis factor receptor (sTNFR2) levels in the sera and nasal epithelial lining fluids (ELF) of 20 patients with Japanese cedar pollinosis from the pre- to the postseason period, and compared the results with those of 10 nonallergic control subjects. The symptom scores of the allergic subjects were significantly (P〈0.01) higher than those of the nonallergic subjects during the early stage and mid-stage of the season. There were no statistical differences between the allergic and nonallergic subjects in the TNF-α levels in sera and ELF from the pre- to the post-season. In the allergic subjects, however, the levels of sTNFR1 and sTNFR2 in ELF were significantly elevated during the early stage (P〈0.05) and mid-stage (P〈0.01) of the season, whereas those in sera did not change from the pre- to the post-season period. The levels of TNF-α in ELF were more than 10 times higher than those in sera, whereas the levels of sTNFR1 and sTNFR2 in ELF were less than half of those in sera in the allergic and nonallergic subjects. These results suggest that sTNFR1 and sTNFR2 may play a role in the pathogenesis of nasal allergic reaction.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2018-07-05
    Description: Asymptomatic versus Symptomatic Patients with Severe Aortic Stenosis Asymptomatic versus Symptomatic Patients with Severe Aortic Stenosis, Published online: 04 July 2018; doi:10.1038/s41598-018-28162-x Asymptomatic versus Symptomatic Patients with Severe Aortic Stenosis
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
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  • 8
    Keywords: CANCER ; GENOME ; PATHWAYS ; THERAPY ; GENE ; BREAST-CANCER ; prognosis ; MUTATION ; DATABASE ; MUTATIONS ; SOMATIC MUTATIONS ; tumours ; Type ; LANDSCAPES ; MYELOID-LEUKEMIA GENOME ; PROJECT ; CANCER-THERAPY ; MANAGEMENT ; SUBTYPES ; REPERTOIRE ; SCIENCE ; development
    Abstract: The International Cancer Genome Consortium (ICGC) was launched to coordinate large-scale cancer genome studies in tumours from 50 different cancer types and/or subtypes that are of clinical and societal importance across the globe. Systematic studies of more than 25,000 cancer genomes at the genomic, epigenomic and transcriptomic levels will reveal the repertoire of oncogenic mutations, uncover traces of the mutagenic influences, define clinically relevant subtypes for prognosis and therapeutic management, and enable the development of new cancer therapies
    Type of Publication: Miscellaneous publication
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  • 9
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  64. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC); 20130526-20130529; Düsseldorf; DOCP 054 /20130521/
    Publication Date: 2013-05-22
    Keywords: chronic post-traumatic headach ; mild traumatic brain injury ; treatment ; ddc: 610
    Language: English
    Type: conferenceObject
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  • 10
    ISSN: 0303-7207
    Keywords: Cloning ; DNA sequence ; Pig ; Pituitary hormone ; Pro-opiomelanocortin ; mRNA
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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