Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Collection
Publisher
Years
  • 1
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to try the application of autologous cultured dermal substitute (CDS) in conjunction with patient's own epidermis. The autologous CDS was prepared by seeding cultured autolougus fibroblasts on the spongy matrix of hyaluronic acid and atelo-collagen. A 9-year-old man with a giant congenital pigmented nevus (intradermal type) was included in this clinical study. A part of nevus (30 cm × 10 cm) was excised superficially at a thickness of 20/1000 inches, and followed by second excision to remove nevus at a level of full-thickness skin. The autologous CDS was applied to the debrided wound surface. The split-thickenss skin obtained by first excision was preserved at 4 °C. After 1 week, the epidermis was obtained from the preserved split-thickness skin using dispase, and followed by grafting on the wound bed, which was prepared by applying autologous CDS. This patient's own epidermis was found to take permanently, achieving an excellent clinical results.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: Recently, various types of cultured skin substitutes have been developed and some of them are used clinically. This study was designed to evaluate the efficacy of allogenic cultured dermal substitute which was applied to burn injuries in clinical trials such as deep dermal burns and dermal burns. Methods: Allogenic cultured dermal substitute (CDS) was simply applied to the burn wound, over which covering materials were applied to protect CDS. Results: The application of CDS to deep dermal burns was proved to facilitate healthy granulation tissue formation at early stage and epithelialization from the outer margins. When CDS applied to the debrided wound surface of dermal burns, an excellent wound bed was generated which was suitable for the graft take of an autologous patch. Conclusion: CDS provides an excellent epithelialization and granulation for burn wounds.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 12 (2004), S. 0 
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The authors have developed a CDS by culturing fibroblasts on the two-layered spongy matrix of hyaluronic acid (HA) and atelo-collagen (Col). This CDS is designed to promote wound healing by synergistic effect of fibroblasts and matrix. Both HA and Col molecules seem to function biologically in the process of wound healing. HA molecules play a critical role in several cellular functions such as migration and proliferation by promoting adhesion and disadhesion between the cell and the tissue substrate. Besides providing structural support and strength to the new tissue, Col molecules have a profound effect on the cells within and on its matrix. Col and Col-derived peptides act as chemoattractants for fibroblasts in vitro and may have a similar activity in vivo. Fibroblasts seeded on the Col surface of two-layered spongy matrix were found to attach, proliferate, and release vascular endothelial growth factor (VEGF) as well as fibronectin. The cryopreserved CDS was found to keep the original potency to release VEGF after thawing followed by re-culturing. Multi-center's clinical research using allogeneic CDS has been proceeded as a national millennium project for regenerative medicine. These products are able to be stored in a freezer and transported to other hospitals in a frozen state. The clinical evaluation involving 180 cases has been already conducted using allogeneic cryopreserved CDS at 30 hospitals across Japan since April 2001. The results obtained in our clinical study suggest that this type of allogeneic CDS is able to provide an effective therapy for patients with severe full-thickness skin defects. These excellent clinical evaluations seem to be closely related to the results obtained in this fundamental study, especially related to the potency of cryopreserved allogeneic CDS to release VEGF and fibronectin.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: A treatment of extensive burn contracture in children needs to repeat the autologous skin graft. This study was designed to evaluate the application of autologous CDS to prepare the proper wound bed acceptable for the split-thickness autologous skin graft. Methods: Prior to the clinical study, the master cell banking system was established using a small piece of skin derived from each patient. The autologous CDS was prepared by plating the patient's own fibroblasts, cultured from the master cells, on a spongy matrix of hyaluronic acid and atelo-collagen. The CDS was applied on the skin defects left behind surgical excision to release scar contracture. The split-thickness autologous skin graft (6∼8/1000 inch) was applied on the wound bed, prepared by using the CDS. Results: The clinical trials were conducted in 5 cases. When the autologous CDS was applied on the skin defect, exposing subcutaneous fatty tissue, the highly vascularlized wound bed was prepared within about 1 week. Although a split-thickness skin graft was very thin, the severe contracture was not observed over a period of several months. Conclusion: The application of autologous CDS is promising for the treatment for extensive burn scar contracture in children.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: Multi-center's clinical study for the application of the cultured dermal substitute (CDS) composed of hyaluronic acid and collagen spongy matrix with allogenic cultured fibroblasts1 is in progress since 2001. In this study, effect of the CDS for the treatment of fresh DDB wounds was tested. Methods: Six cases of second-degree burns, diagnosed as DDB by observation of blood stream of the dermal capillary using Compact Micro Vision System (Hi-Scope®, Hi Rox Co., Japan), were treated with the cultured allogenic fibroblasts, CDS. Results: The epithelialization of the wounds was obtained on 8.2 ± 2.6 (mean ± SD) days after the application. Conclusion: The results of our clinical experiences suggest that the allogenic cultured fibroblasts have beneficial effect on the wound healing of the DDB.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: Clinical researches using allogeneic CDS, developed in the R & D Center for Artificial Skin of Kitasato University, have been carried out in 30 medical centers across Japan. The clinical results in our hospital, especially focusing on the treatment of refractory ulcers and dermal burns, were reported in this study. Methods: The CDS was prepared by plating cultured fibroblasts on a spongy matrix of hyaluronic acid and atelo-collagen. The CDS was used in 13 clinical trials, including 6 refractory ulcers, 4 dermal burns, 1 skin defect left behind preparing skin flaps, and 2 skin defects left behind removal of scar. The use of CDS in conjunction with a conventional ointment-gauze dressing was repeated at an interval of 4 to 7 days over a period of 2 to 6 weeks. Results: The successful application with CDS was achieved in all cases. Especially, in case of refractory ulcers, failed to heal even by using trafermin (Fiblast spray®) or other ointments, a complete healing was achieved in one case and wound size reduction was observed in other 5 cases within 6 weeks. Conclusions: Our results suggest that the CDS is effective in the treatment of refractory ulcers and other skin defects. The excellent clinical results seem to be related to the cytokines released from the CDS.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: Multi-center clinical trials were performed using allogeneic cultured dermal substitutes (CDS) newly developed in the R & D Center for Artificial Skin of Kitasato University. Method: This study was designed to evaluate the efficacy of CDS for the treatment of burns in 5 cases and the treatment of skin ulcers in 4 cases. Complications with the skin ulcers were diabetes (1 case), varix (1 case), and collagen diseases (2 cases). The wound surface area was measured with picture analysis software during the wound healing process. Results: The clinical evaluation was made using a protocol that includes a standard for the development of new wound dressings. According to the comprehensive judgment, the results achieved were evaluated as excellent for 4 burn cases and good for one case. Regarding the skin ulcers, results were excellent in 3 cases and good for one case. No harmful complications were observed during this study. Conclusions: CDS were useful for burn and ulcer treatment but the wound surface must be checked rigorously for the occurrence of infection during the healing process.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...