Blackwell Publishing Journal Backfiles 1879-2005
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Process Engineering, Biotechnology, Nutrition Technology
: Conversion of isoflavones from glycosides to aglycones in SoyLife and soymeal using varying concentrations of β-glycosidase, and different pH conditions and temperatures was investigated. The best conditions for the conversion of glycosides to aglycones were pH 5.0, 50 °C, and 5 h incubation with 5 units β-glycosidase/g Soy Life and 1.5 units/g soymeal. Under these conditions, the amount of genistein, daidzein, and glycitein in Soy Life treated with β-glycosidase were 4.22, 11.52, and 11.85 μmol/g compared to untreated controls of 0.26, 0.97, and 4.43 μmol/g, respectively. In soymeal, the amounts were 3.21, 2.02, and 2.12 μmol/g compared to untreated controls of 1.23, 1.25, and 1.51 μmol/g, respectively. Mole percent recovery of genistein was 87% in Soy Life and 80% in soymeal, respectively.
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