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  • 1
    ISSN: 0378-1119
    Keywords: RNA splicing ; Recombinant DNA ; benomyl ; complementation ; intron ; microtubules
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0378-1119
    Keywords: Ca^2^+/calmodulin-dependent protein phosphatase 2B ; FKBP12 ; Saccharomyces cerevisiae ; TOR proteins ; amiloride ; cyclophilin ; cytoplasmic signal transduction ; immunosuppressive drugs ; rapamycin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; conservation of intron positions ; fungal genetics ; sequence homologies
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0983
    Keywords: Yeast ; Galactokinase ; Mutant selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The galactose analogue 2-deoxy-galactose (2DG) has been widely used to select for mutations in the gene encoding the galactose pathway enzyme galactokinase (GalK). We have tested the effect of 2DG on Candida albicans to see if it could be used to obtain GalK- mutants in this diploid asexual yeast. 2DG was shown to be toxic to wild-type cells. Enzyme assays demonstrated that 2DG can induce GalK as efficiently as galactose. Examination of the initital rate of galactose uptake indicated that the galactose transport system is constitutive. 2DG-resistant mutants were isolated from mutagenized cultures and shown to have very low levels of GalK activity. The potential genetic applications of this system of direct mutant selection are discussed.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0983
    Keywords: Double stranded RNA ; Restriction/modification ; Ustilago ; Virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Specific exclusion relations are know among the three Ustilago maydis viruses that are associated with the cytoplasmically transmitted ‘killer phemomenon’. Of the three viruses P1, P4 and P6, only P1, and P4 cancoexist in one host cell. Mutual exclusion occurs between P1 and P6 and P4 unilaterally excludes P6. The exclusion relations were originally defined among the wild-type viruses. Those relations can be modified by two specific segments that are a part of the P4 dsRNA genome and were also found in some sensitive strains that contained part of the viral genome. Also, deletion of the dsRNA segment that is assumed to encode the toxin information permits the formation of hybrid genomes that otherwise cannot be formed. The data is interpreted in terms of a dsRNA restriction modification system in which the killer toxin or a toxin-linked function acts as the restriction factor and segments H3 and H4 or H4 alone contain the necessary information for the modification of certain sites on the M and L segments of the P1 and P4 viruses but not on the P6 segments.
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  • 6
    ISSN: 1432-0983
    Keywords: dsRNA viruses ; Ustilago maydis ; Virus exclusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three dsRNA virus complexes determine the expression of 3 distinct proteinaceous toxins by cells of Ustilago maydis. These toxins affect sensitive cells of the same species and related species. Based on the expression of the viral coded toxins and the dsRNA content of progeny from crosses between strains each expressing a different toxin specificity exclusion relations were noticed and defined in earlier studies. A restriction modification type mechanism operating in the exclusion process was compatible with the genetic data obtained but a second mechanism based on replication competition between the dsRNA molecules was not ruled out by these studies. The reported study provides support for the latter model based on examination of the fate of toxin expression in haploid vegetative cells resulting from heterokaryons formed between strains expressing different toxin specificities. In these vegetative cells dual specificities were detected in some instances and their segregation was examined. The results suggest that the exclusion of the toxin coding segments is related to their size and affinity to the replicase but some effects are exerted also by other dsRNA segments of the virus complex.
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  • 7
    ISSN: 1432-0983
    Keywords: Meiosis ; Hydroxyurea ; Mutations ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of exposure of nuclei at different stages of meiosis to hydroxyurea (HU) was tested by examination of its effect on the frequency of auxotrophic mutations and on intergenic recombination. The results indicate that recombination is increased most significantly if the nuclei are exposed to the drug during the premeiotic-S phase and to a lesser extent if exposed during prophase. Similarly, nuclei exposed to HU during premeiotic-S phase and prophase mutate at a frequency 10 to 50 times higher than untreated meiotic nuclei. The degree of response of the mutagenic events and recombination events is not similar and it is suggested that the mutagenic events occur at stages prior to recombination. The temporal relations of the two events in response to HU suggest that the mutagenic events precede recombination and the results obtained with HU only amplify the mutagenic events that normally occur in meiotic nuclei and are related to the meiotic effect.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 98 (1974), S. 301-310 
    ISSN: 1432-072X
    Keywords: Meiosis ; Synaptinemal Complex ; Schizophyllum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Meiosis in Schizophyllum commune is asynchronous. Various stages of the first meiotic division can be detected. The only stage in which the bivalents are spread sufficiently is metaphase I. Based on counts in metaphase I it is proposed that n=8. The synaptinemal complex of Schizophyllum consists of a banded lateral component with a 240 Å repeating unit. The dimensions of the complex are similar in size to that found in Boletus. Fibrils fill the central region. The complex is often found in association with the nuclear membrane.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 91 (1973), S. 179-182 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The detection of chlamydospores of Schizophyllum commune in liquid medium is described. The short thick walled cells are formed by intercalary septation which leads also to modification of the septal complex. The chemical composition of the cell walls of chlamydospores is similar to the composition of the vegetative mycelium.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-072X
    Keywords: Killer phenomenon ; dsRNA ; Segmented genome ; Ustilago maydis ; Encapsidation ; Fungal virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From earlier studies with the Ustilago maydis virus and other dsRNA viruses it is known that discrete dsRNA segments typical of each virus are obtained by extraction. A variation exists with respect to the encapsidation of these segments among different viruses. The encapsidation of the genome in individual particles of the Ustilago virus was examined by electron microscopy after disruption of virus particles. The study included the P6 wild-type and 2 mutants containing only part of the genome. The results indicate that most virus particles of the wild-type and the mutants contain up to 12–14×106 daltons of dsRNA. Since the largest extracted molecule is 3.2×106D these findings suggest that an individual particle may contain more than one segment of dsRNA. Free linear molecules that exceed in size the extracted segments were also found following the disruption of each of the 3 virus types examined. Thus, the viral genome seen segmented after extraction is organized as a concatamer in the capsid and each virus particle can contain an entire viral genome consisting of each type of the segments seen after extraction, a repeat of a single segment or a random assortment. In each case the information may be organized as a concatamer.
    Type of Medium: Electronic Resource
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