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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  127. Kongress der Deutschen Gesellschaft für Chirurgie; 20100420-20100423; Berlin; DOC10dgch340 /20100517/
    Publication Date: 2010-05-17
    Keywords: ddc: 610
    Type: conferenceObject
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  • 2
    Keywords: Germany ; NETWORK ; NETWORKS ; SYSTEM ; DISEASE ; DISEASES ; GENOME ; PROTEIN ; PROTEINS ; DNA ; murine ; FAMILY ; OPEN READING FRAME ; SEQUENCE ; virus ; IDENTIFICATION ; HERPES-SIMPLEX-VIRUS ; EPSTEIN-BARR-VIRUS ; HUMAN CYTOMEGALOVIRUS ; SINGLE ; GENE-PRODUCT ; Epstein-Barr virus ; SAN-FRANCISCO ; FAMILIES ; LIBRARIES ; C-ELEGANS ; interaction ; NUCLEAR LAMINA ; CORE ; MUTATIONAL ANALYSIS ; SCREEN ; LIBRARY ; SARCOMA-ASSOCIATED HERPESVIRUS ; USA ; INTERACTION MAP ; cytomegalovirus ; SIMPLEX-VIRUS TYPE-1 ; virology ; GENOMES ; Herpes simplex virus 1 ; SPREAD ; DNA VIRUSES ; COVERAGE ; EGRESS ; MAREKS-DISEASE VIRUS
    Abstract: Herpesviruses constitute a family of large DNA viruses widely spread in vertebrates and causing a variety of different diseases. They possess dsDNA genomes ranging from 120 to 240 kbp encoding between 70 to 170 open reading frames. We previously reported the protein interaction networks of two herpesviruses, varicella-zoster virus (VZV) and Kaposi's sarcoma-associated herpesvirus ( KSHV). In this study, we systematically tested three additional herpesvirus species, herpes simplex virus 1 (HSV-1), murine cytomegalovirus and Epstein-Barr virus, for protein interactions in order to be able to perform a comparative analysis of all three herpesvirus subfamilies. We identified 735 interactions by genome-wide yeast-two-hybrid screens (Y2H), and, together with the interactomes of VZV and KSHV, included a total of 1,007 intraviral protein interactions in the analysis. Whereas a large number of interactions have not been reported previously, we were able to identify a core set of highly conserved protein interactions, like the interaction between HSV-1 UL33 with the nuclear egress proteins UL31/UL34. Interactions were conserved between orthologous proteins despite generally low sequence similarity, suggesting that function may be more conserved than sequence. By combining interactomes of different species we were able to systematically address the low coverage of the Y2H system and to extract biologically relevant interactions which were not evident from single species
    Type of Publication: Journal article published
    PubMed ID: 19730696
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  • 3
    Keywords: RECEPTOR ; CANCER ; CELLS ; ENDOTHELIAL-CELLS ; EXPRESSION ; Germany ; IN-VIVO ; VIVO ; liver ; GENE ; GENE-EXPRESSION ; DIFFERENTIATION ; ACTIVATION ; CUTTING EDGE ; INFECTION ; INDUCTION ; DENDRITIC CELLS ; T-CELLS ; TOLERANCE ; bone marrow ; BONE-MARROW ; STIMULATION ; MOUSE ; DELIVERY ; CLONAL EXPANSION ; VIRAL-INFECTION ; CROSS-PRESENTATION ; endothelial cells ; EFFECTOR FUNCTION ; RIG-I ; T cell immunity ; HEPATITIS-B VIRUS ; MURINE CYTOMEGALOVIRUS-INFECTION ; TOLEROGENIC DENDRITIC CELLS
    Abstract: BACKGROUND & AIMS: Dendritic cell activation through ligation of pattern recognition receptors leading to full functional maturation causes induction of CD8(+) T-cell immunity through increased delivery of costimulatory signals instead of tolerance. Here we investigate whether organ-resident antigen-presenting cells, such as liver sinusoidal endothelial cells (LSECs), also switch from tolerogenic to immunogenic CD8(+) T-cell activation upon such stimulation. METHODS: Murine LSECs were isolated by immunomagnetic separation and analyzed for functional maturation upon triggering pattern recognition receptors or viral infection employing gene expression analysis and T cell coculture assays. In vivo relevance of the findings was confirmed with bone-marrow chimeric animals. RESULTS: LSECs expressed numerous pattern recognition receptors that allowed for sentinel function, but ligand-induced activation of these receptors was not sufficient to overcome tolerance induction of CD8(+) T cells. Importantly, viral infection with murine cytomegalovirus caused functional maturation of antigen-presenting LSECs and was sufficient to promote antigen-specific differentiation into effector CD8(+) T cells in the absence of dendritic cells and independent of CD80/86. CONCLUSIONS: These results shed new light on the generation of organ-specific immunity and may contribute to overcoming tolerance in relevant situations, such as cancer
    Type of Publication: Journal article published
    PubMed ID: 19737567
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  • 4
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Guinea pigs show an increased cell-mediated immunity against host cell antigens after active immunization with vaccinia virus infected tissue culture cells. This immunologic adjuvant effect was observed in a heterologous system comprising primary rabbit kidney cells, primary fibroblasts from the mouse, permanent monkey kidney cells andin vivo infected mouse brain cells. The quantitative determination of the cellular immune response was carried out both in macrophage migration inhibition and in lymphocyte transformation tests. Cell-mediated immunity appears on the third day after immunization. A prerequisite for the adjuvant effect is the reproduction of the virus in the cell against which the increased cell-mediated immune response is directed.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Cell surface alterations induced by vaccinia virus can be demonstrated either by the cytocidal effect of specific antibodies against vaccinia virus on the infected cells or agglutination of the infected cells by Concanavalin A. This change occurs 2–3 hours after infection, that is before any mature particles of vaccinia virus were produced. It coincides with an increased immunogenicity of the host cell. The production of cytotoxic antibodies against host cell antigens was also increased after active immunization with plasma membranes isolated from vaccinia virus infected BHK-cells, whereas the fraction of cell nuclei did not show up this effect. The cytotoxic antibodies which proved to be 7 S immunoglobulines, are specifically directed against BHK-cells as could be shown by absorption tests with membranes of BHK-cells.
    Notes: Zusammenfassung Vacciniavirusinduzierte Zelloberflächenveränderungen können durch den zytoziden Effekt virusspezifischer Antikörper auf infizierte Zellen sowie durch die Agglutination virusinfizierter Zellen durch Concanavalin A demonstriert werden. Diese Veränderungen treten zeitlich korreliert mit einer gesteigerten Immunogenität der Wirtszelle 2–3 Stunden nach Infektionsbeginn bereits vor der Produktion reifer infektiöser Viruspartikeln auf. Eine gesteigerte Antikörperproduktion gegen Wirtszellantigene wird auch nach Immunisierung mit isolierten Plasmamembranen vacciniavirusinfizierter Zellen erreicht, während Zellkernfraktionen diesen Effekt nicht auslösen. Bei den zytotoxischen Antikörpern, die zu den 7 S-Immunglobulinen gehören, handelt es sich — wie durch Absorption mit BHK-Zellmembranen gezeigt werden kann — um spezifisch gegen BHK-Zellen gerichtete Antikörper.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The action of peritoneal exudate cells (PEC) from normal and vaccinia virus infected mice on infectious vaccinia virus particles was investigatedin vitro. PEC from immune mice showed a significantly higher infectivity titre reduction (virus clearance, VC) than normal cells. This effect could be clearly attributed to the macrophage. Vaccinia virus multiplied in PEC from normal animals while there was no virus propagation in cells from immunized mice. The release of adsorbed or engulfed virus was reduced significantly in PEC from immunized animals. Anti-vaccinia-antibodies seem to activate normal macrophages to increased virus clearance. This stimulating effect was demonstrable only in the IgG fraction of the antiserum. The activity of macrophages from mice injected three times over a period of 14 days with vaccinia virus could be entirely blocked with anti-mouse-IgG, while PEC from mice injected one time six days previously were not inhibited.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 267 (1977), S. 160-163 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Separation of Sendai virus envelope protein on SDS-polyacrylamide gels. Sendai virus (8 mg ml"1) in phosphate-buffered saline without Mg or Ca (PBS-A), 1.5 xlO5 haemag-glutinating units (HAU) per ml were treated with 0.25% NP 40 for 15 min at 20 C (ref. 6). The suspension was centrifuged at ...
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 255 (1975), S. 552-554 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Primary embryonic fibroblasts of the strains C3H (H-2kk), DBA/2 (H-2dd) and the (C3HxDBA/2)Fi generation of the two strains (H-2kdkd) were prepared, infected with vaccinia virus and used as targets for virus-specific cell mediated cytotoxicity. Lymphocytes were collected from mice sensitised 6d ...
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 257 (1975), S. 596-597 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Alteration of H-2 antigens was studied by immunofluo-rescence. L-929 cells after 1 h infection with 10 TCID50 per cell vaccinia virus strain WR and 19 h incubation in serum-free medium, as well as normal L-929 cells were stained by indirect immunofluorescence. L cells (5 x 106) were incubated in ...
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  • 10
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The action of Sendai virus specific cytolytic T lymphocytes (CTL) against Sendai virus infected macrophages was found to be H-2 restricted while Sendai virus infected cell lines, including fibroblasts and tumour cells, were lysed across the H-2 barrier to some extent. The properties of the Meth-A tumour cell, which was resistant to lysis by allogenic killer cells was investigated. Sendai virus specific CTL failed to kill Sendai virus infected Meth-A cells but after vaccinia virus infection these target cells were susceptible to lysis by vaccinia virus specific CTL.
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