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  • 1
    Keywords: Microbiology ; Microbiology ; Springer eBooks
    Description / Table of Contents: PhageFISH for Monitoring Phage Infections at Single Cell Level -- Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria -- Engineering Bacteriophage-Based Biosensors -- Introduction of Phage Genome into Escherichia coli by Electroporation -- Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1 -- Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA -- Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography -- Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes -- Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis -- Analyzing Phage-Host Protein-Protein Interactions using Strep-tag® II Purifications -- Techniques to Assess Phage/Biofilm Interaction -- Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages -- The Use of the greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for the Analysis of Bacterial Pathogenesis -- Use of a Silkworm Larva Model in Phage Therapy Experiments -- Exploitation of Drosophila Infection models to Evaluate Antibacterial Efficacy of Phages -- Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems -- Quantitating Phage Efficacy in ready-To-Eat Meats -- Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy
    Abstract: This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including ‘duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems’. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both established and novice phage scientists
    Pages: XI, 216 p. 81 illus., 46 illus. in color. : online resource.
    ISBN: 9781493989409
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  • 2
    Keywords: Life sciences ; Microbiology ; Life sciences ; Microbiology ; Springer eBooks
    Description / Table of Contents: Basic Phage Mathematics -- Analysis of Host-Takeover during SPOi Infection of℗ Bacillus subtilis -- Practical Advice on the One-Step Growth Curve -- Iron Chloride Flocculation of Bacteriophages from Seawater -- Purification of Bacteriophages using Anion-Exchange Chromatography -- Encapsulation Strategies of Bacteriophages (Felix O1) for Oral Therapeutic Application -- Encapsulation of℗ Listeria℗ Phage A511 by Alginate to Improve its Thermal Stability -- Application of a Virucidal Agent to Avoid Over-Estimation of Phage Kill during Phage Decontamination Assays on Ready-to-Eat Meats -- Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes -- Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology -- Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing -- Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques using SISPA -- Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque -- Preparing cDNA Libraries from Lytic Phage Infected Cells for Whole Transcriptome Analysis by RNA-Seq -- Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis -- Annotation of Bacteriophage Genome Sequences using DNA Master: An Overview -- Phage Genome Annotation using the RAST Pipeline -- Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams.-℗ Transposable Bacteriophages as Genetic Tools -- Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes -- Use of RP4::mini-Mu for Gene Transfer -- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu
    Abstract: This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III℗ is a valuable resource for both established and novice phage scientists
    Pages: XI, 316 p. 61 illus., 36 illus. in color. : online resource.
    ISBN: 9781493973439
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  • 3
    Call number: QH506:60/501
    Keywords: Bacteriophages / physiology ; Bacteriophages / isolation & purification ; Genomics / methods
    Pages: xxii, 307 p. : ill.
    ISBN: 9781588296825
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  • 4
    ISSN: 1432-072X
    Keywords: Spirochaeta aurantia ; Glycogen ; Spirochetes ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracts of Spirochaeta aurantia contained granules approximately 36 nm in diameter. These granules were purified by isopycnic centrifugation on CsCl gradients and shown on the basis of chemical and spectroscopic evidence to be glycogen. Electron microscopic cytochemical methods revealed glycogen-like granules in S. aurantia cells.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The addition of biogenic (acetate and butyrate) or abiogenic (propionate, isobutyrate, and valerate) volatile fatty acids to theClostridium acetobutylicum growth medium reduced the lag phase. Propionate and valerate were reduced to the corresponding alcohols (l-propanol andl-pentanol). Dicarboxylic acids were not metabolized, but reduced glucose utilization and solvent synthesis. A hydroxyacid, 4-hydroxybutyric acid, was quantitatively converted to 1,4-butanediol.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract When cast in a polyacrylamide gel, whole lipopolysaccharide (LPS) and the lipid A fraction of LPS fromSalmonella typhimurium andEscherichia coli O111∶B4 reacted with the silver stain described by Tsai and Frasch [11]. However, the polysaccharide fractions released from the LPS by acid hydrolysis were not stained. This is inconsistent with the previously believed notion that the polysaccharide component is that which reacts with the silver stain.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The technique of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate the O-antigen of three strains ofPseudomonas aeruginosa, two strains, ofSalmonella typhimurium, and one strain ofEscherichia coli. No significant difference in separation and migration rate of sample was seen at the various acrylamide gel concentrations used. However, samples electrophoresed through acrylamide running gels at pH 6.8 migrated faster and the resolution of the high-molecular-weight O-antigen bands was greater than of the samples separated in gels at pH 8.8. On the basis of our observations, we could conclude that separation of the heterogeneous O-antigen in SDS-PAGE is probably due to differences in their charge densities and their molecular sizes. Also, pH 6.8 resolving gels are especially useful in the separation of high-molecular-weight O-antigen for epitope mapping by reaction with monoclonal antibodies in Western immunoblotting.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0197-8462
    Keywords: electromagnetic field ; protein synthesis ; Escherichia coli ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Escherichia coli JM83 {F- ara Δ(lac-proAB) rpsL [φ80dΔ(lacZ)M15]} in midlog growth phase at 30 °C were exposed to 60 Hz sinusoidal magnetic field of 3 mT of nonuniform diverging flux, inducing a nonuniform electric field with a maximum intensity of 32 μV/cm using an inductor coil. Exposed and unexposed control cells were maintained at 30.8 ± 0.1 °C and 30.5 ± 0.1 °C, respectively. Quadruplicate samples of exposed and unexposed E. coli cells were simultaneously radiolabeled with 35S-L-methionine at 10 min intervals over 2 hr. Radiochemical incorporation into proteins was analyzed via liquid scintillation counting and by denaturing 12.5% polyacrylamide gel electrophoresis. The results showed that E. coli exposed to a 60 Hz magnetic field of 3 mT exhibited no qualitative or quantitative changes in protein synthesis compared to unexposed cells. Thus small prokaryotic cells (less than 2 μm × 0.5 μm) under constant-temperature conditions do not alter their protein synthesis following exposure to 60 Hz magnetic fields at levels at 3 mT. © 1994 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A bacterium, as yet unidentified, has been isolated from floor dust by direct selection on minimal agar using l-glucitol (d-gulitol) as the sole carbon energy source. The bacterium possesses a constitutive enzyme which catalyzes the reaction: l-glucitol + NAD+→d-sorbose + NADH + H+. A new species of enzyme has been induced by l-arabinitol or ribitol, but not l- or d-glucitol, and the induction is only partially counteracted by the glucose-repression effect. The constitutive enzyme was purified by fractionation on Sephadex G-200 gel and chromatography on DEAE Biogel A. The enzyme required NAD+, but not NADP+, as a cofactor. It oxidizes also ribitol, xylitol and l-arabinitol, but not d-arabinitol, lactitol or a variety of other commercially available alditols. The enzyme is not inhibited by 10 mM sodium azide but is totally inhibited by 0.1 mM potassium ferricyanide.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspension and high voltage electroporation. We have obtained frequencies as high as 5.8 × 103 transformants/g of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa.
    Type of Medium: Electronic Resource
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