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  • 1
    ISSN: 0168-583X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0375-9474
    Keywords: Nuclear reaction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Experimental Gerontology 3 (1968), S. 197-198 
    ISSN: 0531-5565
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In the present study, the mode of cell death induced by highly toxic trialkylated tin compounds has been evaluated. Treatment of undifferentiated HL-60 cells with submicromolar to micromolar concentrations of tri-n-butyltin (TBT) led only to a slight decrease in cell viability measured with trypan blue exclusion. Nevertheless, cell membrane blebbing was observed by means of light microscopy and condensation of nuclear chromatin and formation of apoptotic bodies was demonstrated in Hoechst 33342 stained cells. The nuclear chromatin condensation was associated with an extensive DNA fragmentation. Visualized by agarose gel electrophoresis, genomic DNA appeared as a characteristic ladder-like pattern of DNA fragments which is the biochemical hallmark of apoptosis. The typical internucleosomal DNA digestion was concentration-dependent and began within 2 to 3 h of incubation. During the incubation period a persistent and steady elevation of intracellular free calcium concentration ([Ca2+]i) could be detected. Furthermore, the chromatin condensation and DNA fragmentation could be blocked by supplementation of the incubation medium with zinc pointing to an activation of a zinc-sensitive and calcium-dependent endogenous endonuclease. Higher concentrations of tributyltin (≥ 5 μmol/L TBT) led within hours to a cell killing with degenerative changes indicative of necrosis, demonstrated by plasma membrane disruption which was accompanied by random DNA breakdown. Furthermore, these concentrations also provoked a persistent elevation in [Ca2+]i which reached, even after 10 min, higher levels in comparison with apoptosis-inducing concentrations. The loss in membrane integrity observed at these concentrations of TBT could be due to an activation of calcium-dependent phospholipases. Here it is shown that activation of cytosolic phospholipase A2 (cPLA2) leads to liberation of arachidonic acid (AA) out of the phospholipid membrane. The results presented here demonstrate that organometals are able to induce different cell death pathways depending on the applied concentration: low concentrations led to apoptosis whereas high concentrations stimulate necrosis. We suggest that there exists a direct correlation between the intracellular free calcium concentration and the mode of cell death.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 339 (1965), S. 1-9 
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary By means of the dynamic elastometer ofkrug, it could be shown that the white matter of 31 brains from diabetic patients was significantly firmer than that of 60 control brains. The gray matter of 28 brains from uremic patients revealed a significant reduction in firmness. In the diabetic patients a close relationship existed between the individual values for the rigidity of the brain and the blood sugar values. Only a slight reduction of the dry substance was found in the brains from diabetic patients. Many factors play a role in the pathogenesis of changes in diabetes, such as disturbances of cellular and capillary membranes, and changes of the colloidal state by polymerization of the substances in the white matter. Here either increased amounts of polymerizable substances are deposited and polymerized by catalyzers within the brain, or polymerizable substances of the brain are polymerized by pathologic products arising out of the intermediate metabolism.
    Notes: Zusammenfassung Gegenüber 60 Vergleichsfällen konnte mittels des dynamischen Elastometers nachKrug bei31 Diabetikergehirnen bestätigt werden, daß die weiße Substanz signifikant fester ist. Die graue Substanz von 28 Urämiegehirnen zeigte eine signifikante Verringerung der Starre. Zwischen den Einzelwerten der Starre und dem Blutzucker Hegt bei den Diabetesfällen eine straffe Korrelation vor. Eine Verminderung der Trockensubstanz fand sich zwar bei den Diabetikergehirnen, aber nur mit geringer Signifikanz. Bei der Pathogenese der Veränderungen beim Diabetes spielen wohl viele Faktoren eine Rolle, wie Störungen an der Zell- und Capillarmembran, sowie Änderungen des kolloidalen Zustandes durch Polymerisation von Stoffen im Mark, wobei entweder polymerisationsfähige Stoffe vermehrt abgelagert und durch hirneigene Katalysatoren polymerisiert oder hirneigene polymerisationsfähige Stoffe durch pathologische Produkte aus dem Zwischenstoffwechsel polymerisiert werden.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 51 (1985), S. 19-23 
    ISSN: 1432-0584
    Keywords: Pesticides ; Carbamates ; DDT ; Platelets ; Prostaglandins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Aggregation of human platelets was investigated after pre-incubation of platelet rich plasma with various pesticides of the carbamate type. Whereas N-methyl-carbamates (insecticides) inhibited the arachidonic acid induced aggregation, N-phenyl-carbamates (herbicides) had no effect. The influence of the different carbamates on aggregation coincided with their inhibition of thromboxane B2-formation. — DDE, a metabolite of the insecticide DDT, affected aggregation to a similar extent as N-methyl-carbamates. DDT, however, had no inhibitory activity.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 49 (1980), S. 123-132 
    ISSN: 1432-0533
    Keywords: Brain tumours ; Flow-through cytophotometry ; DNA content of tumour cell nuclei ; Ploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The flow-through cytophotometric method was used to investigate the single-cell DNA content in 60 tumours of the CNS and allied structures (9 meningiomas, 5 ependymomas, 15 astrocytomas, 11 anaplastic astrocytomas, 8 glioblastomas, 7 medulloblastomas, 2 oligodendrogliomas, 1 anaplastic oligodendroglioma, and 2 neurinomas). The cytophotometric parameters were correlated with morphological and clinical data of the tumours. The results are summarised in Tables 1–7. With regard to the DNA content of their cell nuclei, the gliomas present a behaviour similar to tumours in other organs, but in the present study the cytophotometric signs of malignancy in gliomas are not so evident as in carcinomas or sarcomas. The information obtained by flow cytophotometric methods may be helpful in diagnosing the degree of malignancy in brain tumours.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Inflammation research 34 (1991), S. 261-263 
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Murine progressive ankylosis (MPA) is characterized by periarticular ossification and joint ankylosis. We studied calcergy and calciphylaxis in MPA. Calcergy represents a chemical attraction betwen heavy metal salts and apatite followed by hydroxyapatite deposition around collagen Mice were injected subcutaneously with FeCl2, PbAcetate, and saline, and sacrificed at intervals between 5 minutes and 14 days. Section of skin and subcutaneous tissues were stained with Von Kossa's stain. No mice developed calcifylaxis. PbAcetate produced calcergy in all mice. MPA mice exhibited calcergy qualitatively and quantitatively the same as normal mice suggesting abnormal calcergy is not responsible for joint ankylosis in MPA.
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  • 9
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die katamnestische Auswertung von 120 flowcytometrisch untersuchten Mammakarzinomen mit 5 Jahren Nachbeobachtungszeit ergab eine Verschlechterung der Prognose in der Reihenfolge: diploide-polyploide-aneuploide Tumoren. Prognostisch schlecht ist auch eine hoher Anteil von S- und G2M-Phasenanteilen bei diploiden. Tumoren, Sinngemäß gleiche Zusammenhänge ergaben sich zu folgenden Befunden bei der Operation, deren prognostische Bedeutung bekannt ist wie: Tumorgröße, Lymphknotenmetastasierung und histologisches Grading. Der mittlere DNS-Gehalt/Zellkern und die mittlere Färbeintensität der Zellkerne, gemessen durch Einzelzytophotometrie haben ebenfalls prognostische Bedeutung. Insgesamt haben zytophotometrische Untersuchungen einen prognostischen Wert, der sich auch statistisch sichern läßt.
    Notes: Summary Based on flow cytometry (120 cases) and scanning cytophotometry (56 cases) prognosis turns to the worst in the sequence diploid-polyploid-aneuploid tumors and with increasing S- and G2M-compartments. Principally the same correlation exists to other prognostic signs as lymphatic metastases, tumor size and histological grading.
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Electrochimica Acta 13 (1968), S. 2203-2205 
    ISSN: 0013-4686
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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