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  • 1
    Keywords: Life sciences ; Immunology ; Biochemistry ; Cell Biology ; Life sciences ; Cell Biology ; Immunology ; Animal Biochemistry ; Springer eBooks
    Description / Table of Contents: Autophagy (Autophagia) from 1860 to 1963 -- History of Autophagy after 1963 -- Signalling in Autophagy -- Autophagosome Formation -- Physiological Role of Autophagy and Implications in Disease -- Studying autophagy: List of useful antibodies produced via a community effort
    Abstract: This book provides an expert summary of autophagy, a relatively new but rapidly expanding field of biomedical science with important implications in health and disease. After a historical review ranging up to the identification of autophagy genes in mammals, the authors discuss the signaling pathways that regulate autophagy, the mechanism of autophagosome formation and the physiological roles of autophagy in development, ageing, neurodegeneration, immune function and cell differentiation. A comprehensive list of useful antibodies for studying autophagy compiled as a community effort is included at the end. The book is intended for newcomers to the field, as well as more experienced researchers looking for a condensed but comprehensive introduction to the physiological function and regulation of the autophagic pathway in mammalian organisms
    Pages: VI, 103 p. 15 illus., 12 illus. in color. : online resource.
    ISBN: 9783319331454
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  • 2
    Keywords: Biochemistry ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Recombinant Expression, Purification and Assembly of p62 Filaments -- Structural Studies of Autophagy-related Proteins -- Structural Studies of Mammalian Autophagy Lipidation Complex -- Structural Studies of Selective Autophagy in Yeast -- Biophysical Studies of LC3 Family Proteins -- Reconstituting Autophagy Initiation from Purified Components -- Cell-free Reconstitution of Autophagic Membrane Formation -- Use of Peptide Arrays for Identification and Characterization of LIR Motifs -- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System -- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature -- Studies of Receptor-Atg8 Interactions during Selective Autophagy -- Correlative Light and Electron Miscopy of Autophagosomes -- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells -- 3-color Simultaneous Live Imaging of Autophagy-related Structures -- Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy -- Methods for Imaging Autophagosome Dynamics in Primary Neurons -- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinsoń€™s Disease Research -- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo -- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells -- Measurement of Bulk Autophagy by a Cargo Sequestration Assay -- Methods to Detect Loss of Lysosomal Membrane Integrity -- ℗Ư℗ƯMonitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress -- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches -- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen -- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- Methods for the Study of Entotic Cell Death -- MHC Class I Internalization via Autophagy Proteins -- Analysis of Autophagy for Liver Pathogenesis -- Autophagy in 3D In Vitro and Ex Vivo Cancer Models -- Autophagy in Platelets -- Methods to Image Macroautophagy in the Brain In Vivo -- Measuring Nonselective and Selective Autophagy in the Liver -- Measuring Autophagy in Pancreatitis -- Characterization of the €œáutophagic flux́€ in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy -- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing -- Investigating Non-selective Autophagy in Drosophila -- Imaging the Dynamics of Mitophagy in Live Cells -- Triggering Mitophagy with Photosensitizers -- Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC -- Assays to Monitor Mitophagy in Drosophila -- Mitophagy Dynamics in Caenorhabditis elegans -- Methods for Studying Mitophagy in Yeast -- Measuring Anti-bacterial Autophagy -- Quantitative Phosphoproteomics of Selective Autophagy Receptors -- Analysis of Chaperone-mediated Autophagy -- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations
    Abstract: This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XVII, 741 p. 137 illus., 99 illus. in color. : online resource.
    ISBN: 9781493988730
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 356 (1992), S. 344-346 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To test the effects of activators of trimeric G proteins on the action of brefeldin A(BFA), we used a perforated cell system17 that allows cytosolic proteins to be completely washed away from cellular membranes18. In this reconstituted system, exogeneously added cytosol, GTP and an ...
    Type of Medium: Electronic Resource
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