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  • 1
    Publication Date: 2006-04-21
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 2
    Keywords: CELLS ; EXPRESSION ; CELL ; Germany ; human ; THERAPY ; EXPOSURE ; LONG-TERM ; GENE ; GENE-EXPRESSION ; gene therapy ; TRANSDUCTION ; gene transfer ; GENE-TRANSFER ; MARKER ; BIOLOGY ; TARGET ; virus ; DELETION ; VECTOR ; PROMOTER ; NUMBER ; PROMOTERS ; genetics ; EFFICIENT ; REPLICATION ; GENE-THERAPY ; RETROVIRAL VECTORS ; CONSTRUCTION ; heredity ; FELINE FOAMY VIRUS ; ARRAY ; TERMINAL GAG DOMAIN ; PROFILES ; ENV LEADER PROTEIN ; UBIQUITIN ; RETROVIRUS ; ACCESSORY BET PROTEIN ; biosafety ; deficient vector ; lacZ ; NOD/SCID-REPOPULATING CELLS ; SIN vector ; UBIQUITIN-C
    Abstract: As serious side effects affected recent virus-mediated gene transfer studies, novel vectors with improved safety profiles are urgently needed. In the present study, replication-deficient retroviral vectors based on feline foamy virus (FFV) were constructed and analyzed. The novel FFV vectors are devoid of almost the complete env gene plus the internal promoter - accessory bel gene cassette including the gene for the viral transcriptional transactivator Bel1/Tas. In these Bel1/Tas-independent vectors, expression of the lacZ (beta-galactosidase) marker gene is directed by the heterologous, constitutively active human ubiquitin C promoter (ubl). Env-transcomplemented vectors have unconcentrated titers of more than 10(5) transducing units/ml. The vectors allow efficient transduction of a broad array of diverse target cells, which can be increased by repeated vector exposure. However, the number of lacZ marker gene expressing cells decreased slightly upon serial passages of the transduced cells. Vectors carrying a self-inactivating (SIN) deletion of the TATA box and most parts of the viral promoter were not rescued by wt FFV whereas those with the intact or a partially deleted promoter were readily reactivated. This finding indicates that the viral promoters are in fact non-functional, pointing to a highly advantageous safety profile of these new FFV-ubi-lacZ-SIN vectors
    Type of Publication: Journal article published
    PubMed ID: 17203107
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Der Urologe 39 (2000), S. 362-367 
    ISSN: 1433-0563
    Keywords: Schlüsselwörter Nierenzellkarzinom ; Metastasen ; Chirurgie ; Key words Renal cell carcinoma ; Metastases ; Surgery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Renal cell carcinomas (RCC) are endowed with impressive metastatic potential. Patients with RCC who present with apparently solitary metastatic lesions represent a small cohort. Due to the different long-term results achieved with surgical resection of metastatic RCC lesions, a generally accepted judgement about this treatment modality is not possible. Several studies suggest that aggressive surgical management can provide an effective treatment, especially in patients with solitary pulmonary metastases. Most noteworthy is the fact that because of the organ distribution of RCC metastases, surgical therapy is dominated by general and thoracic surgeons, neurosurgeons, and orthopaedic surgeons. Therefore, an interdisciplinary approach is one of the most important key points for a successful outcome in these patients.
    Notes: Zusammenfassung Das Nierenzellkarzinom (NZK) ist durch eine ausgeprägte Tendenz zur Metastasierung charakterisiert, wobei solitäre Metastasen eine Minderheit darstellen. Die sehr unterschiedlichen Resultate der operativen Therapie von NZK-Metastasen erlauben keine pauschale Beurteilung dieser Vorgehensweise, sondern erforderen eine differenzierte Betrachtung der einzelnen Organsysteme. Dabei werden die besten Langzeitresultate verbunden mit einer akzeptablen Morbidität nach der Resektion von Lungenmetastasen erzielt. Entsprechend dem Verteilungsmuster der Metastasenlokalisationen wird die chirurgische Therapie des disseminierten NZK wesentlich durch die Nachbardisziplinen Allgemein- und Thoraxchirugie, Neurochirurgie und Orthopädie dominiert. Eine enge interdisziplinäre Zusammenarbeit, z. B. im Rahmen eines Tumorzentrums, ist daher für ein erfolgreiches chirurgisches Behandlungskonzept unerlässlich.
    Type of Medium: Electronic Resource
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