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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  95. Jahrestagung der Vereinigung Südwestdeutscher Hals-Nasen-Ohrenärzte; 20110916-20110917; Heidelberg; DOC11hnosw12 /20110711/
    Publication Date: 2011-07-11
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 2
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Jahrestagung der Gesellschaft für Medizinische Ausbildung - GMA; 20081002-20081005; Greifswald; DOC08gma40 /20080819/
    Publication Date: 2008-08-20
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 3
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Jahrestagung der Gesellschaft für Medizinische Ausbildung - GMA; 20081002-20081005; Greifswald; DOC08gma59 /20080819/
    Publication Date: 2008-08-20
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 4
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Zeitschrift für Medizinische Ausbildung; VOL: 29; DOC47 /20120515/
    Publication Date: 2012-05-16
    Description: Background: Following recent modifications of the Medical Licensure Act (ÄApprO) in the year 2009, palliative care was introduced as a compulsory 13th cross-disciplinary subject (Q13) in the undergraduate curriculum. Its implementation must have taken place before the beginning of the final year (´practical year´) in August 2013 and has to be substantiated for the medical exams taking place in October 2014. Very diverse structures pertaining to palliative care teaching were described in previous surveys at various medical faculties in Germany. As a result, the current and future plans and concepts related to content and exams of a mandatory Q13 course at the respective faculty sites should be ascertained. Methods: Since 2006, the German Medical Students' Association (bvmd) has been carrying out a bi-annual survey at all medical faculties in Germany regarding the current situation of teaching in the field of palliative care. After designing and piloting an online survey in May 2010, a one-month online survey took place. The data was assessed using a descriptive approach. Results: 31 of 36 medical faculties took part in the survey. At the time of questioning, 15 faculties already taught courses according to the requirements of the new ÄApprO; at three sites the Q13 is yet to be introduced commencing in 2012. A teaching curriculum for Q13 already existed at 15 faculty sites, partly based on the curricular requirements of the German Association for Palliative Medicine (DGP). Six sites described an implementation process as yet without an independent curriculum. Most of the faculties aim for 21-40 course hours, which will for the most part be provided as lectures, seminars or less often in more assisted and intense formats. The majority of the participating faculties intend an examination containing multiple choice questions. At 8 universities there is an independent Chair for palliative medicine (5 more are planned); this was linked with a higher degree of mandatory teaching in alignment with the requirements of the ÄApprO. A broad spectrum of educationally-involved occupational groups, specialist disciplines and external co-operating partners, were mentioned. Conclusion: The infrastructural prerequisites of the present curricular concepts and the degree of implementation of the Q13 according to the requirements of the new ÄApprO diverge significantly among the various medical faculties. The efforts made to produce a qualitatively high standard of teaching with regard to the multifaceted questions concerning the support for severely and terminally ill patients is as much reflected in the survey, as the special implications of an independent Chair for palliative medicine for the implementation of the requirements by law. The participation of various occupational groups in this survey as well as the broad spectrum of those involved highlights the interdisciplinary and multi-professional dimension of teaching in palliative care.
    Description: Fragestellung: Mit der Änderung der ärztlichen Approbationsordnung (ÄApprO) im Jahre 2009 wurde die Palliativmedizin als 13. Querschnittsfach (Q13) verpflichtend in das studentische Curriculum eingeführt. Die Umsetzung dieser Änderung muss bis zum Beginn des Praktischen Jahres im August 2013 oder bei der Meldung zum Zweiten Abschnitt der Ärztlichen Prüfung für den Prüfungstermin im Oktober 2014 erfolgen.In vorangegangenen Umfragen wurden an den medizinischen Fakultäten in Deutschland sehr heterogene palliativmedizinische Lehrstrukturen beschrieben. Daher sollten nun die curricularen und prüfungsbezogenen Planungen der jeweiligen Fakultäten zur Implementierung eines verpflichtenden Q13 Palliativmedizin erfragt werden.Methodik: Die Bundesvertretung der Medizinstudierenden Deutschlands (bvmd) führt seit 2006 zweijährliche Umfragen an allen medizinischen Fakultäten in Deutschland zum gegenwärtigen Stand der palliativmedizinischen Lehre durch.Nach Konzeption eines Online-Fragebogens und Pilotierung erfolgte im Mai 2010 eine einmonatige Online-Umfrage. Die Daten wurden deskriptiv ausgewertet.Ergebnisse: 31 von 36 medizinischen Fakultäten nahmen an der Befragung teil. An 15 Fakultäten existierten zum Befragungszeitpunkt bereits verpflichtende Lehrveranstaltungen entsprechend der Vorgabe der neuen ÄApprO; an drei Standorten soll das Q13 Palliativmedizin erst ab dem Sommersemester 2012 verpflichtend eingeführt werden. An 15 Fakultäten bestand bereits ein Lehrcurriculum für Q13, teils angelehnt an die curricularen Vorgaben der Deutschen Gesellschaft für Palliativmedizin. Sechs Standorte beschrieben einen begonnenen Implementierungsprozess noch ohne Curriculum. An den meisten Fakultäten werden 21-40 Lehrveranstaltungsstunden angestrebt, die überwiegend in Form von Vorlesungen oder Seminaren, seltener auch in betreuungsintensiveren Formaten angeboten werden sollen. Die Mehrzahl der teilnehmenden Fakultäten strebt eine Prüfung in Form von Multiple Choice Fragen an. An 8 Universitäten bestand ein eigenständiger Lehrstuhl für Palliativmedizin (5 weitere in Planung); dies war mit einem höheren Maß an Pflichtlehre analog der Vorgabe der ÄApprO verbunden. Ein breites Spektrum an lehrbeteiligten Berufsgruppen, Fachdisziplinen und externen Kooperationspartnern wurde angegeben.Schlussfolgerung: Die infrastrukturellen Voraussetzungen, die bisherigen curricularen Planungen und der Grad der Implementierung des Q13 analog der Vorgabe der neuen ÄApprO divergieren zwischen den einzelnen medizinischen Fakultäten erheblich. Die Bemühungen um eine qualitativ hochwertige Lehre in Hinblick auf die vielschichtigen Fragestellungen bei der Betreuung schwerst- und sterbenskranker Patienten spiegeln sich in der Umfrage ebenso wider wie der besondere Stellenwert eines eigenständigen Lehrstuhls für Palliativmedizin für die Umsetzung der gesetzlichen Vorgaben. Die Mitwirkung der verschiedenen Berufsverbände an dieser Umfrage sowie das große Spektrum der Lehrbeteiligten unterstreicht die interdisziplinäre und multiprofessionelle Dimension palliativmedizinischer Lehre.
    Keywords: Palliative care ; cross-disciplinary subject ; implementation process ; curriculum ; examinational didactics ; Palliativmedizin ; Querschnittsfach 13 ; Implementierungsprozess ; Curriculum ; Prüfungsdidaktik ; ddc: 610
    Language: German
    Type: article
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  • 5
    Abstract: Fluorescence-labeled peptide-MHC class I multimers serve as ideal tools for the detection of antigen-specific T cells by flow cytometry, enabling functional and phenotypical characterization of specific T cells at the single cell level. While this technique offers a number of unique advantages, MHC multimer reagents can be difficult to handle in terms of stability and quality assurance. The stability of a given fluorescence-labeled MHC multimer complex depends on both the stability of the peptide-MHC complex itself and the stability of the fluorochrome. Consequently, stability is difficult to predict and long-term storage is generally not recommended. We investigated here the possibility of cryopreserving MHC multimers, both in-house produced and commercially available, using a wide range of peptide-MHC class I multimers comprising virus and cancer-associated epitopes of different affinities presented by various HLA-class I molecules. Cryopreservation of MHC multimers was feasible for at least 6 months, when they were dissolved in buffer containing 5-16% glycerol (v/v) and 0.5% serum albumin (w/v). The addition of cryoprotectants was tolerated across three different T-cell staining protocols for all fluorescence labels tested (PE, APC, PE-Cy7 and Quantum dots). We propose cryopreservation as an easily implementable method for stable storage of MHC multimers and recommend the use of cryopreservation in long-term immunomonitoring projects, thereby eliminating the variability introduced by different batches and inconsistent stability.
    Type of Publication: Journal article published
    PubMed ID: 25297339
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  • 6
    Abstract: BACKGROUND & AIMS: We report a novel experimental immunotherapeutic approach in a patient with metastatic intrahepatic cholangiocarcinoma. In the 5year course of the disease, the initial tumor mass, two local recurrences and a lung metastasis were surgically removed. Lacking alternative treatment options, aiming at the induction of anti-tumor T cells responses, we initiated a personalized multi-peptide vaccination, based on in-depth analysis of tumor antigens (immunopeptidome) and sequencing. METHODS: Tumors were characterized by immunohistochemistry, next-generation sequencing and mass spectrometry of HLA ligands. RESULTS: Although several tumor-specific neo-epitopes were predicted in silico, none could be validated by mass spectrometry. Instead, a personalized multi-peptide vaccine containing non-mutated tumor-associated epitopes was designed and applied. Immunomonitoring showed vaccine-induced T cell responses to three out of seven peptides administered. The pulmonary metastasis resected after start of vaccination showed strong immune cell infiltration and perforin positivity, in contrast to the previous lesions. The patient remains clinically healthy, without any radiologically detectable tumors since March 2013 and the vaccination is continued. CONCLUSIONS: This remarkable clinical course encourages formal clinical studies on adjuvant personalized peptide vaccination in cholangiocarcinoma. LAY SUMMARY: Metastatic cholangiocarcinomas, cancers that originate from the liver bile ducts, have very limited treatment options and a fatal prognosis. We describe a novel therapeutic approach in such a patient using a personalized multi-peptide vaccine. This vaccine, developed based on the characterization of the patient's tumor, evoked detectable anti-tumor immune responses, associating with long-term tumor-free survival.
    Type of Publication: Journal article published
    PubMed ID: 27397612
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  • 7
  • 8
    Abstract: BACKGROUND: Validated assays are essential to generate data with defined specificity, consistency, and reliability. Although the process of validation is required for applying immunoassays in the context of clinical studies, reports on systematic validation of in vitro T cell assays are scarce so far. We recently validated our HLA-peptide multimer staining assay in a systematic manner so as to qualify the method for monitoring antigen-specific T cell responses after immunotherapy. METHODS: Parameters of the assay, specificity, precision, linearity, sensitivity, and robustness were assessed systematically. Experiments were designed to address specifically each parameter and are detailed. RESULTS: Nonspecific multimer staining was below the acceptance limit of 0.02% multimer((+)) CD8((+)) cells. The assay showed acceptable precision in all dimensions it was repeated (CV 〈 10%) and also demonstrated a linear detection (R(2) 〉 0.99) of antigen specific cells. CONCLUSIONS: We succeeded in validating the HLA-multimer staining assay in a systematic manner. Additionally, we propose a technical framework and recommendations that can be applied for validating other T cell assessment methods. (c) 2016 International Clinical Cytometry Society.
    Type of Publication: Journal article published
    PubMed ID: 27363684
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  • 9
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  Mainz//2011; 56. Jahrestagung der Deutschen Gesellschaft für Medizinische Informatik, Biometrie und Epidemiologie (gmds), 6. Jahrestagung der Deutschen Gesellschaft für Epidemiologie (DGEpi); 20110926-20110929; Mainz; DOC11gmds022 /20110920/
    Publication Date: 2011-09-20
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 10
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  80. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie; 20090520-20090524; Rostock; DOC09hnod578 /20090417/
    Publication Date: 2009-04-25
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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