Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Les auteurs ont effectué un travail destiné à évaluer la possibilité du passage d'axones de régénération au travers d'autogreffes de nerfs dévitalisés depuis un certain temps, comparativement à celui d'autogreffes nerveuses fraîches. Dans ce but, une expérimentation a été menée sur deux groupes de rats blancs “Sprague Dawley”. Dans un des deux groupes, on a sectionné les nerfs sciatiques droits et on y a ensuite greffé les segments distaux de nerfs sciatiques gauches prélevés quinze semaines auparavant. Dans l'autre groupe, on a greffé sur les nerfs sciatiques droits sectionnés des segments prélevés sur les nerfs sciatiques gauches intacts. Après 8 semaines, on a comparé dans les deux groupes les axones myélinisés à trois endroits différents des nerfs sciatiques droits greffés: l'extrémité distale du segment proximal, le milieu de la greffe et l'extrémité proximale du segment distal. Les résultats ont montré que la capacité du nerf dégénéré à laisser passer les axones en régénérescence est 0.79 fois inférieure à celle du nerf frais (p[t]〈0.05). Cependant la différence n'est pas très importante. Ceci signifie que des nerfs dégénérés peuvent être utilisés commes greffes complémentaires lorsqu'un grand nombre de greffes nerveuses autogènes est nécessaire, comme c'est le cas pour le traitement chirurgical des lésions du plexus brachial.
    Notes: Summary We have compared the passage of regenerating axons through old degenerated nerve autografts and fresh nerve autografts in two groups of Sprague Dawley rats. In one group the right sciatic nerve was divided and repaired with the graft taken from the distal segment of the left sciatic nerve, which had been severed 15 weeks before. In the other group the right sciatic nerve was repaired with the graft taken from the intact left sciatic nerve. Eight weeks later the myelinated axons were counted at three different locations in the grafted right sciatic nerve in both groups. The old degenerated nerve autografts were less effective in allowing the passage of regenerating axons than the fresh nerve autografts, but the difference was not great. This provides justification for the use of old degenerated nerve as an additional graft where a large number of autogenous nerve grafts are required, such as in the operative treatment of injuries to the brachial plexus.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When anti-CPV.VP2 monoclonal antibody was employed in immunofluorescence staining, an intense signal was observed within BmK1-Parvo-infected Bm5 cells but not within uninfected cells or cells infected with a wild-type BmNPV-K1. In hemagglutination assay, the expression level of VP2 were 3.2 × 103 HA units/ml from infected Bm5 cells, 2.1× 105 HA units/larvae from infected larval fat body, and 1.6× 106 HA units/ml from infected larval hemolymph. These results suggested that BmNPV vector system using B. mori larva as host could be applied to efficient mass-production of recombinant vaccines.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Bombyx mori nucleopolyhedroviruses (BmNPVs), isolated from a sericultural Korean farm, were purified and characterized by their DNA restriction pattern, virus replication, polyhedra production and gene structures. The EcoR I and Sal I fragments showed similar overall patterns with minor difference but distinguishable patterns in each isolate. There was no significant difference in the virus replication pattern, yield of total polyhedra production and polyhedra morphology, but the yield of released polyhedra by BmNPV-K1 in Bm5 cells was 2 to 5 times higher than that of other isolates. In comparative studies of p10 gene, BmNPV-K1 and K3 had same structure and they encoded a protein consisting of 94 amino acids. Although BmNPV-K2 encoded the same length of amino acids with BmNPV-K1 and K3, it had different structure, and BmNPV-K4 had the p10 gene encoding 70 amino acids.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1432-0428
    Keywords: Insulin resistance ; lipolysis ; free fatty acids ; glycerol ; glucose clamp technique ; glucose production ; glucose utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Although extensive evidence indicates that free fatty acids can decrease glucose utilization in vitro, it is still controversial how an increase in lipolysis affects glucose metabolism in man. To test the hypothesis that an increase in lipolysis is related to insulin resistance, we examined the effect of lipid-heparin infusion on glucose metabolism in ten normal subjects by the euglycaemic glucose clamp technique and isotopic determination of glucose turnover. In the control euglycaemic clamp studies with insulin infusion at 0.2 and 1.0 mU·kg−1·min−1, endogenous glucose production was suppressed from the basal rate of 2.0±0.3 mg· kg−1min−1 to 1.1±0.7 mg·kg−1·min−1 and -0.4±0.7mg· kg−1min−1 respectively. Glucose utilization increased from the basal rate of 2.0±0.3 mg·kg−1min−1 to 2.3±0.5mg· kg−1min−1 and 5.9±1.8 mg·kg−1min−1 respectively. When the euglycaemic clamp studies were coupled with lipid-heparin infusion at comparable low and high rates of insulin infusion, endogenous glucose production increased (1.8± 0.7 mg·kg−1·min−1, p〈0.001, and 0.3±0.6 mg·kg−1· min−1, p〈0.05, respectively), and glucose utilization decreased (2.1±0.3 mg·kg−1·min−1, not significant, and 3.2±0.7 mg·kg−1·min−1, p〈0.001 respectively). These data suggest that the artificial induction of intravascular lipolysis by lipid-heparin infusion leads to a state of insulin resistance in man.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1432-0428
    Keywords: Keywords Short stature ; gestational diabetes mellitus ; prevalence ; heterogeneity.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined the associations between demographic characteristics including short stature and the prevalence of gestational diabetes mellitus (GDM) in Korean women. In this study, a total of 9005 pregnant women underwent universal screening for GDM. Oral glucose tolerance tests (100 g OGTT) were performed in positive screenees (1 h plasma glucose ≥ 7.2 mmol/l) and GDM was diagnosed using National Diabetes Data Group criteria. Women with GDM were older and heavier than those with a positive screen and normal OGTT, as well as those with a negative screen. However, height of women with GDM was significantly shorter than those with a positive screen and normal OGTT, and a negative screen. When the study subjects were stratified according to height quartiles, the plasma glucose at the screening test decreased as height increased. Furthermore, the prevalence of GDM was highest in the shortest quartile ( ≤ 157 cm) group; the odds ratio for GDM was two times greater compared with the highest quartile ( ≥ 163 cm) group, even after controlling for age and body mass index (BMI). In addition, multiple logistic regression analysis revealed that greater prepregnancy BMI, age, weight gain, a parental history of diabetes mellitus, and shorter maternal height were directly and independently associated with the prevalence of GDM. We have found that short stature is an independent risk factor for GDM in the racially homogenous population of Seoul, Korea. It is suggested that this propensity may be conveyed primarily by environmental influences. However, genetic factors may also modify the response to the environmental insult. Our findings also emphasize the heterogeneity of factors which predispose to GDM. [Diabetologia (1998) 41: 778–783]
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1434-601X
    Keywords: 11.30.Hv ; 21.10.Dr ; 12.40.Aa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We investigate the density dependence of the neutronproton mass difference using the Nambu and Jona-Las-inio model in combination with the Isgur-Karl constituent quark model. The decrease of the constituent quark masses with increasing density reduces then-p mass difference in the proper way to help resolving the Nolen-Schiffer anomaly. We point out however, that in the presence of vector interactions, this effect is less pronounced than previously suggested.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In order to remove the radiotoxic nuclides, Cs+ and I−, from low-level liquid wastes, the adsorption characteristics have been studied using a mixed adsorbent of chabazite zeolite and activated carbon. The equilibrium data of each nuclide were well correlated with the DA equation in the wide range of equilibrium concentrations. The SEM-EDAX analysis provided precise understanding of the adsorption mechanism of each nuclide. A surface diffusion model was applied to estimate the intraparticle mass transfer and provided prediction results acceptable for practical implementation in the liquid waste treatment.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 25 (1988), S. 515-519 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Retention/structure relationships ; Retention prediction ; Substituted phenols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A retention prediction system (RPS) for substituted phenols in reversed-phase HPLC using a ternary mobile phase was investigated. The RPS was used to predict the retention times of the phenols, and then evaluated by comparing measured and predicted retention data. Excellent agreement between both values was obtained. In addition, the retention mechanism of the phenols was investigated by means of a correlation analysis relating the parameters of the RPS to the physicochemical properties of the phenols. The three properties investigated (partition coefficient, hydrophobic substituent constant and Hammett's constant) were used to describe quantitatively the structure-retention relationship. Significant correlating equations between these descriptors and the retention data were obtained.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 1612-1112
    Keywords: Micellar electrokinetic chromatography ; Systematic optimization ; Flavonoid separation ; Micropreparative MEKC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple and rapid systematic optimization scheme was described for the micellar electrokinetic chromatographic separation of a group of flavonoids. The scheme employed an interpretative optimization approach to predict the optimum conditions for the separation of a group of flavonoids by micellar electrokinetic chromatography. By performing a set of nine pre-planned experiments conducted over the maximum working range for the system, global optimum separation conditions could be determined. To validate the optimization procedure, additional experiments were performed using the optimum experimental conditions derived from the optimization scheme. The results showed that satisfactory separation of all the peaks could be obtained. In addition, the application of the method in micropreparative micellar electrokinetic chromatography of the flavonoids was demonstrated.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 1612-1112
    Keywords: Solid phase extraction ; Optimization ; Orthogonal array design ; Organochlorine pesticides ; Natural waters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A two-level orthogonal array design (OAD) to optimize the solid phase extraction of organochlorine pesticides is described. Parameters including eluting solvents, SPE cartridges packed with C18-bonded silica from different manufacturers, duration of air drying, pH, salinity and humic acid have been examined using OAD. This systematic approach was then used to optimize the relevant parameters required for off-line solid phase extraction of organochlorine pesticides from water. The optimized parameters were employed to perform extraction of the pesticides from natural waters.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...