Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Abstract The non-ionic surfactant Brij-35 can easily be oxidized in solution by dissolved oxygen upon light radiation during its storage. Various factors, such as light radiation, temperature, pH, ionic strength as well as dissolved oxygen influencing the stability of Brij-35 were studied. The results showed that light radiation is the most important factor inducing the oxidation by the dissolved oxygen. The oxidized product was confirmed, indirectly, to be the peroxide of Brij-35 based on a number of evidences. The extent of the oxidation can be traced by a peroxidase or hemin catalyzed fluorogenic reaction with p-hydroxyphenylacetic acid as a substrate. The concentration of the peroxide in a Brij-35 solution (0.8%, w/v) stored at room temperature for a month is in the range of 9 × 10–5 mol/L.
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