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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Current Posters in Otorhinolaryngology - Head and Neck Surgery; VOL: 4; DOC32 /20080422/
    Publication Date: 2008-04-22
    Description: Einleitung: Während der Nasenatmung kommt es bei der Inspiration zur Abkühlung, bei der Exspiration zur Erwärmung der Nasenschleimhaut. Mit Hilfe moderner Infrarotthermographiekameras (IR-Kameras) ist es möglich, die Temperaturänderungen im Atemzyklus mit hoher örtlicher und zeitlicher Auflösung zu messen und in einem Falschfarbenbild sichtbar zu machen. Bisher liegen hierzu keine Daten vor. Ziel dieser Untersuchung war die Untersuchung der Naseneingänge mit einer IR-Kamera bei dem Vorliegen verschiedener Nasenpathologien. Methoden: Es kam die IR-Kamera TVS 500 Ex (Fa. Goratec, Erding, Deutschland) zum Einsatz. Die Aufzeichnung der Naseneingänge erfolgte bei den Patienten in Ruheatmung über mehrere Atemzyklen. Dabei wurden folgende endonasale Pathologien untersucht: Septumdeviation, Septumperforation, Polyposis nasi, Zustand nach ausgedehnter NNH-Operation, sowie vor und nach Adenotomie. Ergebnisse: Die entsprechenden endonasalen Pathologien zeigen spezifische Muster in der Temperaturveränderung in den Naseneingänge während der Atemzyklen. Für die Septumdeviation ergaben sich deutliche Seitenunterschiede in der Temperaturverteilung mit geringerer Abkühlung auf der Seite der Deviation. Bei den anderen Pathologien zeigten sich ebenfalls Seitenunterschiede sowie Unterschiede im Temperaturverlauf im Vergleich zu Normalprobanden. Bei der vergleichenden Messung vor und nach Adenotomie zeigte sich eine deutlich gesteigerte Abkühlung postoperativ. Schlussfolgerung: Mit der IR-Kamera lassen sich Temperaturänderungen während des Atemzyklus in hoher zeitlicher und örtlicher Auflösung darstellen. In Abhängigkeit von der untersuchten Pathologie entstehen unterschiedliche Temperaturmuster. Diese Muster hängen von Veränderungen des nasalen Flows ab.
    Keywords: ddc: 610
    Language: German
    Type: article
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  • 2
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  79. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie; 20080430-20080504; Bonn; DOC08hnod578 /20080422/
    Publication Date: 2008-04-21
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Ultramicroscopy 19 (1986), S. 114 
    ISSN: 0304-3991
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General , Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0890-8508
    Keywords: Chlamydia trachomatis ; in situ DNA hybridization ; non-radioactive detection ; urogenital smears
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In tissue of rats with disseminated intravascular coagulation, fibrin microthrombi can be sensitively detected by immunohistochemical methods, using antisera against rat fibrinogen or fibrin monomer. An indirect immunoperoxidase procedure on paraplast-embedded sections yields best results with regard to the morphology of the thrombi and their localization in the tissue. Only fibrillar immunoreactive material, oriented lengthwise in the vessels, should be regarded as microthrombi formed in vivo.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The influence of different fixation methods on the results of immunoperoxydase staining of immunoglobulin and gastrin producing cells in gastric and duodenal mucosa was investigated. An indirect method was used on paraffin sections. It appeared that that fixatives containing sublimate gave the most consistent results, a sublimate-formaldehyde mixture being the best.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2307
    Keywords: Giardiasis ; Immunohistochemistry ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Jejunal mucosa biopsies from non-immune deficient patients with Giardia lamblia infestation were examined and showed three different groups of mucosal changes, distinguishable on morphological and immunohistochemical grounds. In three patients no morphological or immunohistochemical abnormalities were found (group A). In five patients a normal villous architecture was seen. These biopsies had increased numbers of interepithelial lymphocytes and of immunoglobulin containing cells in the lamina propria, with a relative increase of the number of IgA and IgG containing cells (group B). Two patients with a malabsorption syndrome due to giardiasis had marked villous atrophy, documented by morphometric measurements and large numbers of interepithelial lymphocytes and of immunoglobulin containing cells in the lamina propria, especially IgA and IgG (group C). These findings differ considerably from those in patients with immunodeficiency or gluten sensitive enteropathy. This suggests that when villous atrophy of the jejunal mucosa is found immunohistochemistry of Jejunal biopsy specimens may be helpful in the differential diagnosis between mere giardiasis and giardiasis superimposed on immunodeficiency or gluten sensitive enteropathy.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0428
    Keywords: Juvenile diabetes mellitus ; autoantibodies ; fluorescent antibody technique ; prediabetic state ; IgG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Circulating islet cell antibodies (ICA) were present in high frequency (80%) early after diagnosis and decreased in the time course of childhood diabetes mellitus. The complement fixing ability of islet cell antibodies (CF-ICA) in the course of the disease appeared to depend on the titre of ICA: the coefficient of correlation between ICA and CF-ICA titres was 0.79 and all ICA's with a titre over 16 were complement-fixing. Incubating fresh frozen human pancreatic sections thrice rather than once with the children's sera, increased the detectability of complement fixation by a factor 1.4 in all ICA-positive sera. Thus tested, the detection of complement fixation per se did not appear to have a separate pathogenic significance, as the fraction of complement fixing ICA's was almost constant throughout the clinical course. The presence of ICA-IgG subclasses also was dependent on the ICA titre: above a titre of 16 mostly all four subclasses could be detected. Incubating the pancreatic tissue thrice rather than once with ICA-positive sera resulted in enhanced detectability of ICA-IgG1. Early in the course of childhood diabetes, including two prediabetic children, most of the IgG subclasses could be detected in ICA, but after a duration of one year IgG1 alone was mainly seen. In two other children, having a family history of insulin-dependency, restriction to the IgG2 subclass was found.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0711
    Keywords: Chlamydia trachomatis ; CIN
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We found, as have others, a strong correlation between indirect immunofluorescence techniques and cell culture for the diagnosis ofChlamydia trachomatis infection in material obtained from the cervix. Five epithelial cell types indicative ofChlamydia trachomatis infection (indicator cells) were found in smears in patients with positive immunofluorescence. An attempt to diagnoseChlamydia trachomatis infection in 50 routine smears based on the presence of these indicator cells showed false positives and false negatives so that the diagnosis ofChlamydia trachomatis still requires confirmation by immunofluorescence or culture techniques. The indicator cells frequently had the same morphometric features as cells seen with cervical intraepithelial neoplasia, which may explain why some smears revert to normal after patients are given antibiotics.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 17 Patienten mit DH wurden vielfältige Duodenum- und Jejunum-Biopsien verrichtet. Die Dünndarm-Biopsien aller dieser Patienten zeigten histopathologische Änderungen, die cöliakie-ähnlich waren. Vierzehn dieser Patienten hielten eine glutenfreie Diät (GFD) während mehr als 8 Monaten. Bei allen untersuchten Patienten, die Diät hielten, verbesserten sich die Dünndarmerscheinungen. Die Dosierung von Dapsone, nötig für die Kontrolle der Hauterscheinungen, konnte reduziert werden um mehr als 50% bei 4 Patienten, und 5 andere Patienten, die die glutenfreie Diät auch hielten, brauchten diese Dapsone nicht mehr. Die Immunoglobulin enthaltenden Zellen in der Lamina propria wurden gezählt bei 8 Patienten, die nicht eine GFD hielten, bei 6 Patienten mit dieser GFD und bei 8 gesunden Kontroll-Personen. Die Zahl der IgA, IgM und IgG enthaltenden Zellen hat bei den meisten der DH-Patienten, die keine GFD hielten, zugenommen. Die Zahl der IgM enthaltenden Zellen bei den DH-Patienten, die eine GFD hielten, war dieselbe wie die Zahl dieser IgM enthaltenden Zellen bei den gesunden Kontroll-Personen. Aus dieser Untersuchung könnte man schließen, daß Gluten hauptsächlich eine IgM-Stimulierung in der Lamina propria hervorruft.
    Notes: Summary In 17 patients with DH, multiple duodenal and jejunal biopsies were performed. In all patients the small-intestinal biopsy-specimens showed histopathological changes compatible with coeliac disease. Fourteen of the patients maintained a gluten-free diet (GFD) for more than 8 months. The small-intestinal lesions improved in all patients investigated during the GFD. The dosage of Dapsone needed to control the skin lesions could be reduced by more than 50% in 4 patients and the Dapsone could be stopped in 5 other patients on GFD. The immunoglobulin-bearing cells in the lamina propria were counted in 8 patients not on a gluten-free diet, in 6 patients on gluten-free diet, and in 8 healthy controls. The numbers of IgA-, IgM- and IgG-bearing cells were increased in most of the DH patients who were not on a gluten-free diet. The number of IgM-bearing cells in the DH patients who were on a gluten-free diet was the same as that in the control group. This may indicate a mainly IgM response in the lamina propria induced by gluten.
    Type of Medium: Electronic Resource
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