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  • 1
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Introduction to FOXO Biology -- Characterization of microRNAs Regulating FOXO Expression -- Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter -- Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover -- Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity -- Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes -- Characterization of FOXO Acetylation -- Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays -- Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes -- Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters -- RNAi Mediated Silencing of FoxO Factors -- Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO -- High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO -- Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus -- Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology -- Genome-wide Analysis for Identifying FOXO Protein Binding Sites -- Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors -- Following Transcriptome to Uncover FOXO Biological Functions -- Hydra as Model to Determine the Role of FoxO in Longevity -- Genetic Ablation of FOXO in Mice to Investigate its Physiological Role -- Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity -- Analysis of FOXO3a Gene Polymorphism Associated with Asthma
    Abstract: This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs. Chapters guide readers through biochemical and molecular methods, imaging approaches to monitor the subcellular localization of FOXO factors, omics and bioinformatics approaches, different animal models used in FOXO research, and human studies to investigate the role of FOXO factors in human disease and longevity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge FOXO Transcription Factors: Methods and Protocols aims to ensure successful results in the further study of this vital field
    Pages: XII, 270 p. 54 illus., 33 illus. in color. : online resource.
    ISBN: 9781493989003
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  • 2
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Using mRNA differential display, we found that the gene for NAD+-dependent glycerol phosphate dehydrogenase (GPDH; EC 1.1.1.8) is induced in rat brain following seizure activity. Northern blot and in situ hybridization analysis confirmed the differential display results; they also showed, in a separate model of neuronal activation, that after thermal noxious stimulation of the hind-paws, a similar increase in GPDH mRNA occurs in the areas of somatotopic projection in the lumbar spinal cord. Surprisingly, administration of analgesic doses of morphine or the nonsteroidal antiinflammatory drugs aspirin, metamizol (dipyrone), and indomethacin also increased GPDH mRNA levels in rat spinal cord. The opioid receptor antagonist naloxone completely blocked morphine induction of GPDH but had no effect on GPDH induction by noxious heat stimulation or metamizol treatment, implicating different mechanisms of GPDH induction. Nevertheless, in all cases, induction of the GPDH gene requires adrenal steroids and new protein synthesis, as the induction was blocked in adrenalectomized rats and by cycloheximide treatment, respectively. Our results suggest that the induction of the GPDH gene upon peripheral noxious stimulation is related to the endogenous response to pain as it is mimicked by exogenously applied analgesic drugs.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fluxes in amounts of intracellular calcium ions are important determinants of gene expression. So far, Ca2+-regulated kinases and phosphatases have been implicated in changing the phosphorylation status of key transcription factors and thereby modulating their function,. In ...
    Type of Medium: Electronic Resource
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