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  • 1
    Keywords: Biotechnology ; Human Genetics ; Biotechnology ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Microinjection and Micromanipulation: A Historical Perspective -- Production of Transgenic Mice by Pronuclear Microinjection -- Transgene Recombineering in Bacterial Artificial Chromosomes -- Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice -- Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method -- Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer -- Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection -- Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice -- Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique -- Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation -- Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides -- Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection -- The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications -- The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm -- Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line -- Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology -- Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos -- Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells -- Generation of Rabbit Models by Gene Editing Nucleases -- Production of Genetically Engineered Porcine Embryos by Handmade Cloning -- Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production -- Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer -- Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting -- Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes -- Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing -- Microinjection in Zebrafish for Genome Editing and Functional Studies -- Microinjection of Marine Fish Eggs -- Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method -- Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus -- Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function
    Abstract: This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations
    Pages: XIV, 540 p. 134 illus., 96 illus. in color. : online resource.
    ISBN: 9781493988310
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  • 2
    ISSN: 1573-9368
    Keywords: β-casein gene promoter ; early development ; milk‐mZP3 ; mZP3 gene ; transgenic mice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mouse egg zona pellucida glycoprotein mZP3 (∼83 kDa Mr) serves as a species‐specific sperm receptor and acrosome reaction‐inducer during fertilization in mice. These biological activities are dependent on certain mZP3 serine/threonine‐ (O‐) linked oligosaccharides present at the combining‐site for sperm. In an attempt to produce large amounts of biologically active mZP3, we generated several transgenic mouse lines carrying the full‐length mZP3 gene fused to the β‐casein gene promoter and transcription termination sequence. We found that different transgenic mouse lines have different amounts of recombinant mZP3 (∼63 kDa Mr) in milk of lactating females, from ∼0.3 to 3.5 μg/μl of milk. In all cases, purified milk-mZP3 is active as a sperm receptor and acrosome reaction-inducer in vitro. Unexpectedly, we also found that development of litters from these transgenic mice is related to the amount of mZP3 in the mother's milk. In the most extreme case, litters from the highest expressers fail to live beyond about day-7 post partum unless placed immediately after birth with surrogate wild-type mothers. Litters from lower expressers initially display a complex phenotype that includes effects on hair and body growth, but some of the mice survive and, in time, are restored to a wild-type phenotype. These results demonstrate that relatively large amounts of biologically active mZP3 can be produced in transgenic mouse milk for structural and other studies, but that the presence of mZP3 in milk has dramatic developmental effects on litters, ranging from retarded hair and body growth to death of newborn pups.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1040-452X
    Keywords: Xenopus oocyte ; Interspersed RNA ; Translation ; Oligodeoxynucleotides ; RNA binding protein ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: It has been shown that about two thirds of Xenopus oocyte or sea urchin egg cytoplasmic poly(A)+ RNA contains interspersed repetitive sequences. The functional significance of this interspersed RNA has remained unknown. Here the function of a subfamily of interspersed RNA (XR family; McGrew and Richter, 1989: Dev Biol 134:267-270) in Xenopus oocytes was studied. We found that the elimination of T7 XR (one of the two complementary strands of the XR repeat) interspersed RNA by complementary oligodeoxynucleotides significantly inhibited protein synthesis. On the other hand, the injection of in vitro synthesized T7 XR RNA stimulated translation. Moreover, the insertion of the T7 XR RNA sequence into globin mRNA repressed the translation of the globin mRNA. In order to explain these results, we analyzed interactions between the XR interspersed RNA and oocyte proteins. We found that the major XR RNA binding proteins were p56 and p60, which could be the known mRNA “masking” proteins that bind mRNA and inhibit translation. Further, a 42 kD protein has been identified that appears to bind T7 XR RNA relatively specifically, although it interacts with mRNA with a lower affinity. Based on all of these data, we have proposed that interspersed RNA may be involved in regulating translation by competing with mRNA to interact with certain proteins that can regulate translation. © 1995 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2017-10-31
    Description: Telomeric TERB1–TRF1 interaction is crucial for male meiosis Nature Structural & Molecular Biology, Published online: 30 October 2017; doi:10.1038/nsmb.3496 Disrupting the interaction between telomere protein TRF1 with meiosis-specific protein TERB1 impairs the pairing of X and Y chromosomes via their telomere-adjacent pseudoautosomal regions in pachytene, leading to spermatocyte apoptosis and male infertility in mice.
    Print ISSN: 1545-9993
    Electronic ISSN: 1545-9985
    Topics: Biology , Medicine
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