Description / Table of Contents:
Detection of Streptococcus pyogenes Virulence Factors -- Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens -- The emm-cluster Typing System -- Protocols for Tn-seq Analyses in The Group A Streptococcus -- Genetic Manipulation of Group A Streptococcus – Gene Deletion by Allelic Replacement -- Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging -- Whole Genome Sequence Analysis and Population Genomics of Group A streptococci -- Group A Streptococcus Transcriptome Analysis -- Protocol for Proteome Analysis of Group A Streptococcus -- Investigation of Group A Streptococcal Interactions with Host Glycan Structures using High Throughput Techniques: Glycan Microarray Analysis using Recombinant Protein and Whole Cells -- Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins -- Expression and Purification of Collagen-like Proteins of Group A Streptococcus -- Detection of Fibronectin-binding Proteins of Streptococcus pyogenes using Ligand Blot Analysis -- Morphology and Ultrastructure of Group A Streptococcus Biofilms -- Dynamic Interactions of Group A Streptococcus with Host Macrophages -- Identification of Group A Streptococcus-containing autophagosome-like vacuoles -- Flow Cytometry-based Assays to Quantify Complement Deposition and Neutrophil Uptake of Group a Streptococcus -- Live Cell Microscopy and Flow Cytometry to study Streptolysin S Mediated Erythrocyte Hemolysis -- Isolation of Monoclonal Antibodies to Group A Streptococcus Antigens Using Phage Display -- Assays to Analyse Adhesion of Group A Streptococcus to Host Cells -- The use of Galleria mellonella (wax moth) as an Infection Model for Group A Streptococcus -- A Superficial Skin Scarification Method in Mice to Mimic Streptococcus pyogenes Skin Infection in Humans -- A Mouse Nasopharyngeal Colonisation Model for Group A Streptococcus -- Humanised Plasminogen Mouse Model to Study Group A Streptococcus Invasive Disease -- Lacefield Whole Blood Killing Assay to Evaluate Vaccine Efficacy -- An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera -- Validation of Suitable Carrier Molecules and Target Genes for antisense Therapy using Peptide-coupled Peptide Nucleic Acids (PNAs) in Streptococci -- Interfering with the Folding of Group A Streptococcal pili Proteins -- Use of Streptolysin O (SLO) to Study the Function of Lipid Rafts -- Engineering of Group A Streptococcus Isopeptide Bonds into Immunoglobulin-like Protein Domains
This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Group A Streptococcus: Methods and Protocols aims to ensure successful results in the further study of this vital field
XIV, 403 p. 54 illus., 39 illus. in color. : online resource.
1st ed. 2020.