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  • 1
    Keywords: Medicine ; Neurosciences ; Cytology ; Biomedicine ; Neurosciences ; Apoptosis ; Springer eBooks
    Description / Table of Contents: Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons -- Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy -- Flow Cytometric℗ Analysis of DNA℗ Synthesis℗ and Apoptosis℗ in Central Nervous System℗ Using Fresh Cell Nuclei -- Nuclear Signs of Pre-Neurodegeneration -- Multi-Parametric O2℗ Imaging℗ in Three-Dimensional℗ Neural Cell Models℗ with the Phosphorescent Probes -- Calcium Imaging℗ in Neuron Cell Death -- Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis -- Real-Time Visualization of Caspase-3℗ Activation by Fluorescence Resonance Energy Transfer (FRET) -- Design and Cloning of Short Hairpin℗ RNAs℗ (shRNAs) into a Lentiviral Silencing Vector to -- Genomic Analysis Using Affymetrix Standard Microarray℗ GeneChips (169 Format) in Degenerate Murine Retina -- Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis -- High-Throughput℗ Cell Death℗ Assays -- Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death -- In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death -- Laser℗ Microbeam℗ Targeting℗ of Single Nerve Axons℗ in Cell℗ Culture -- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy -- Targeted Toxicants to Dopaminergic Neuronal Cell Death -- Stem Cells, Neural Progenitors, and Engineered Stem Cells -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors℗ for Gene Transfer℗ and Gene Therapy -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon℗ Vectors for Gene Transfer℗ and Gene Therapy -- Bone Marrow Transplantation℗ for Research and Regenerative Therapies in the Central Nervous System -- Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA) -- Generation of Zebrafish℗ Models by CRISPR/Cas9℗ Genome Editing -- In Vivo Assessment of Neuronal Cell Death in Drosophila -- Drosophila℗ Model for Studying Phagocytosis℗ Following Neuronal Cell Death
    Abstract: This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology
    Pages: XIV, 396 p. 82 illus., 48 illus. in color. : online resource.
    ISBN: 9781493921522
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  • 2
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: The Evolution of Immunocytochemistry in the Dissection of Neural Complexity.-n Immunofluorescence℗ and Genetic Fluorescent Labeling℗ Techniques in the Drosophila℗ Nervous System -- Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris -- Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish℗ Brain -- Immunocytochemical℗ Phenotype℗ of Differentiating℗ Neurons.-Immunocytochemical Identification of Mammalian Differentiating Neurons℗ in the Process of Adult℗ Neurogenesis℗ in the Hippocampal Dentate Gyrus -- Identification of Proliferating and Migrating Cells by BrdU℗ and Other Thymidine Analogues. Benefits and Limitations -- Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous System -- In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis℗ and Autophagy -- Immunocytochemical Detection of Intraneuronal AÎø℗ Peptides in Mouse Models of Alzheimer's Disease -- Purification℗ and Characterization of Mouse Olfactory Ensheathing Cells and their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS) -- Immunocytochemistry of Microglial Cells -- Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage -- Quantification of Immunocytochemical Colocalization in Neurons -- Immunogold℗ Electron Microscopic℗ Quantification℗ of Small Molecular℗ Compounds and Proteins℗ at Synapses and Other Neural Profiles -- Combined Immunocytochemistry℗ and Tracing℗ of Neural Connections -- Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing -- Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices -- Combined Immunochemistry and Live-Imaging of Fluorescent Protein Expressing Neurons in Mouse℗ Brain -- Array Tomography: A Novel High-Resolution Immunofluorescence Technique -- Single Molecule Localization Microscopy℗ to Study Neuronal℗ Microtubule Organization -- Studying Membrane Dynamics℗ of Proteins℗ Using Quantum Dots -- Optical℗ Protein℗ Patterning
    Abstract: This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.℗ Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research
    Pages: XIII, 473 p. 95 illus., 77 illus. in color. : online resource.
    ISBN: 9781493923137
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  • 3
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: The Evolution of Immunocytochemistry in the Dissection of Neural Complexity.-n Immunofluorescence℗ and Genetic Fluorescent Labeling℗ Techniques in the Drosophila℗ Nervous System -- Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris -- Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish℗ Brain -- Immunocytochemical℗ Phenotype℗ of Differentiating℗ Neurons.-Immunocytochemical Identification of Mammalian Differentiating Neurons℗ in the Process of Adult℗ Neurogenesis℗ in the Hippocampal Dentate Gyrus -- Identification of Proliferating and Migrating Cells by BrdU℗ and Other Thymidine Analogues. Benefits and Limitations -- Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous System -- In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis℗ and Autophagy -- Immunocytochemical Detection of Intraneuronal AÎø℗ Peptides in Mouse Models of Alzheimer's Disease -- Purification℗ and Characterization of Mouse Olfactory Ensheathing Cells and their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS) -- Immunocytochemistry of Microglial Cells -- Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage -- Quantification of Immunocytochemical Colocalization in Neurons -- Immunogold℗ Electron Microscopic℗ Quantification℗ of Small Molecular℗ Compounds and Proteins℗ at Synapses and Other Neural Profiles -- Combined Immunocytochemistry℗ and Tracing℗ of Neural Connections -- Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing -- Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices -- Combined Immunochemistry and Live-Imaging of Fluorescent Protein Expressing Neurons in Mouse℗ Brain -- Array Tomography: A Novel High-Resolution Immunofluorescence Technique -- Single Molecule Localization Microscopy℗ to Study Neuronal℗ Microtubule Organization -- Studying Membrane Dynamics℗ of Proteins℗ Using Quantum Dots -- Optical℗ Protein℗ Patterning
    Abstract: This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.℗ Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research
    Pages: XIII, 473 p. 95 illus., 77 illus. in color. : online resource.
    ISBN: 9781493923137
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  • 4
    Keywords: Medicine ; Neurosciences ; Biomedicine ; Neurosciences ; Springer eBooks
    Description / Table of Contents: The Evolution of Immunocytochemistry in the Dissection of Neural Complexity.-n Immunofluorescence℗ and Genetic Fluorescent Labeling℗ Techniques in the Drosophila℗ Nervous System -- Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris -- Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish℗ Brain -- Immunocytochemical℗ Phenotype℗ of Differentiating℗ Neurons.-Immunocytochemical Identification of Mammalian Differentiating Neurons℗ in the Process of Adult℗ Neurogenesis℗ in the Hippocampal Dentate Gyrus -- Identification of Proliferating and Migrating Cells by BrdU℗ and Other Thymidine Analogues. Benefits and Limitations -- Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous System -- In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis℗ and Autophagy -- Immunocytochemical Detection of Intraneuronal AÎø℗ Peptides in Mouse Models of Alzheimer's Disease -- Purification℗ and Characterization of Mouse Olfactory Ensheathing Cells and their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS) -- Immunocytochemistry of Microglial Cells -- Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage -- Quantification of Immunocytochemical Colocalization in Neurons -- Immunogold℗ Electron Microscopic℗ Quantification℗ of Small Molecular℗ Compounds and Proteins℗ at Synapses and Other Neural Profiles -- Combined Immunocytochemistry℗ and Tracing℗ of Neural Connections -- Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing -- Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices -- Combined Immunochemistry and Live-Imaging of Fluorescent Protein Expressing Neurons in Mouse℗ Brain -- Array Tomography: A Novel High-Resolution Immunofluorescence Technique -- Single Molecule Localization Microscopy℗ to Study Neuronal℗ Microtubule Organization -- Studying Membrane Dynamics℗ of Proteins℗ Using Quantum Dots -- Optical℗ Protein℗ Patterning
    Abstract: This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods℗ series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.℗ Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research
    Pages: XIII, 473 p. 95 illus., 77 illus. in color. : online resource.
    ISBN: 9781493923137
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  • 5
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Brain-derived neurotrophic factor (BDNF) exerts its trophic effects by acting on the high-affinity specific receptor trkB. BDNF also modulates synaptic transmission in several areas of the CNS, including the spinal cord dorsal horn, where it acts as a pain modulator by yet incompletely understood mechanisms. Spinal neurons are the main source of trkB in lamina II (substantia gelatinosa). Expression of this receptor in dorsal root ganglion (DRG) cells has been a matter of debate, whereas a subpopulation of DRG neurons bears trkA receptors and contains BDNF. By the use of two different trkB antibodies we observed that 7.7% and 10.8% of DRG neurons co-expressed BDNF + trkB but not trkA, respectively, in rat and mouse. Ultrastructurally, full-length trkB (fl-trkB) receptors were present at somato-dendritic membranes of lamina II neurons (rat: 66.8%; mouse: 73.8%) and at axon terminals (rat: 33.2%; mouse: 26.2%). In both species, about 90% of these terminals were identified as primary afferent fibres (PAFs) considering their morphology and/or neuropeptide content. All fl-trkB-immunopositive C boutons in type Ib glomeruli were immunoreactive for BDNF and, at individual glomeruli and axo-dendritic synapses, fl-trkB receptors were located in a mutually exclusive fashion at pre- or postsynaptic membranes. Thus, only a small fraction of fl-trkB-immunoreactive dendrites were postsynaptic to BDNF-immunopositive PAFs. This is the first ultrastructural description of fl-trkB localization at synapses between first- and second-order sensory neurons in lamina II, and suggests that BDNF may be released by fl-trkB-immunopositive PAFs to modulate nociceptive input in this lamina of dorsal horn.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Existence of the gastro-intestinal peptide secretin in the CNS has been a matter of debate, and contrasting results have been reported, altogether indicating that the CNS is not a major site of production of this peptide. A thorough analysis was conducted in brain of transgenic mice in which the expression of the early region of simian virus 40 large T antigen (Tag) is under control of the rat secretin gene promoter. We studied Tag expression in the brains of E14–P90 transgenic mice as well as secretin mRNA and protein expression in transgenic and control CD1 mice at corresponding developmental stages. We show here a perfect correspondence of Tag and secretin mRNA expression in the mesencephalon of transgenic and normal mice between E14 and birth. In embryos, Tag is also expressed in the spinal cord, as well as in several areas of the peripheral nervous system. Localization of Tag in P0–P90 animals becomes restricted to a single compact cellular mass in mesencephalon at the level of the dorsal raphe, raphe magnus and lateral paragigantocellular nuclei. Neurons of these nuclei display secretin mRNA from E14 to birth, in both control CD1 and transgenic mice. Approximately half of these secretin-expressing neurons are immunoreactive for serotonin (5HT) and/or tryptophan hydroxylase. These results demonstrate that the secretin gene is transiently expressed in mouse serotoninergic mesencephalic neurons during development. In addition our data suggest a trophic role for secretin on neurons known to be involved in multiple superior functions in the normal brain, and lost in neurodegenerative disorders.
    Type of Medium: Electronic Resource
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