Description / Table of Contents:
Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons -- Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy -- Flow Cytometric℗ Analysis of DNA℗ Synthesis℗ and Apoptosis℗ in Central Nervous System℗ Using Fresh Cell Nuclei -- Nuclear Signs of Pre-Neurodegeneration -- Multi-Parametric O2℗ Imaging℗ in Three-Dimensional℗ Neural Cell Models℗ with the Phosphorescent Probes -- Calcium Imaging℗ in Neuron Cell Death -- Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis -- Real-Time Visualization of Caspase-3℗ Activation by Fluorescence Resonance Energy Transfer (FRET) -- Design and Cloning of Short Hairpin℗ RNAs℗ (shRNAs) into a Lentiviral Silencing Vector to -- Genomic Analysis Using Affymetrix Standard Microarray℗ GeneChips (169 Format) in Degenerate Murine Retina -- Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis -- High-Throughput℗ Cell Death℗ Assays -- Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death -- In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death -- Laser℗ Microbeam℗ Targeting℗ of Single Nerve Axons℗ in Cell℗ Culture -- Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy -- Targeted Toxicants to Dopaminergic Neuronal Cell Death -- Stem Cells, Neural Progenitors, and Engineered Stem Cells -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors℗ for Gene Transfer℗ and Gene Therapy -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon℗ Vectors for Gene Transfer℗ and Gene Therapy -- Bone Marrow Transplantation℗ for Research and Regenerative Therapies in the Central Nervous System -- Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA) -- Generation of Zebrafish℗ Models by CRISPR/Cas9℗ Genome Editing -- In Vivo Assessment of Neuronal Cell Death in Drosophila -- Drosophila℗ Model for Studying Phagocytosis℗ Following Neuronal Cell Death
This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology
XIV, 396 p. 82 illus., 48 illus. in color. : online resource.