Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Keywords: CANCER ; POPULATION ; RISK ; PROTEIN ; NF-KAPPA-B ; INFECTION ; GENETIC POLYMORPHISMS ; ASSOCIATION ; polymorphism ; POLYMORPHISMS ; ALPHA ; cytokines ; STAGE ; LESIONS ; NUMBER ; NECROSIS-FACTOR-ALPHA ; HIGH-RISK ; EPITHELIAL-CELLS ; POPULATIONS ; PREVALENCE ; GASTRIC-CANCER ; HELICOBACTER-PYLORI ; TNF-ALPHA ; PRECANCEROUS LESIONS ; CYTOKINE ; REGRESSION ; ASSOCIATIONS ; gastric cancer ; MONOCYTE CHEMOATTRACTANT PROTEIN-1 ; INTERVAL ; HELICOBACTER-PYLORI INFECTION ; odds ratio ; AA ; Helicobacter pylori ; intestinal metaplasia ; premalignant ; stomach cancer ; INTERLEUKIN-1 POLYMORPHISMS ; CAGA PROTEIN ; CHEMOTACTIC CYTOKINES ; CHINESE POPULATION ; premalignant lesions
    Abstract: Helicobacter pylori (HP) infection affects over 50 % of the world's population. The prevalence is over 90% in populations at high risk for gastric cancer, but clinical outcomes of the infection are highly variable and thus host genetic factors have been suggested to play a role in its outcomes in addition to bacterial factors. In this study, we examined the effects of common functional genetic polymorphisms of several proinflammatory cytokines known to be overexpressed in HP-infected gastric mucosa on the risk of various stages of gastric premalignant lesions. The odds ratios (ORs) and 95% confidence intervals (CI) for atrophic gastritis, intestinal metaplasia and dysplasia were estimated by multinominal logistic regression analysis among 2,033 Venezuelan subjects. There was a significant effect of IL8-251A allele on the prevalence of dysplasia (p = 0.021). The OR associated with the A-allele was 1.34 (95% CI: 0.82-2.18) for heterozygotes and 2.00 (95% CI: 1.13-3.56) for homozygotes, compared with the TT genotype. Furthermore, there was a statistically significant interaction between the number of A-alleles and HP cag A genotype (p = 0.009), suggesting that the A-allele increased the risk of dysplasia only when cag A was present. The OR for the AA compared with TT genotype was 3.22 (95% CI: 1.60-6.52) in this group. There were no associations with other proinflammatory cytokines studied, i.e., IL1 beta, 1L6, monocyte chemoattractant protein I (MCP1) and TNF alpha, or with other stages of premalignant lesions. The present study provides important evidence suggesting host-bacterial interactions in the development of gastric precancerous lesions. (c) 2006 Wiley-Liss, Inc
    Type of Publication: Journal article published
    PubMed ID: 16671087
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: CANCER ; EXPRESSION ; MODEL ; MODELS ; POPULATION ; RISK ; GENE ; INFECTION ; RISK-FACTORS ; CARCINOGENESIS ; GENETIC POLYMORPHISMS ; ASSOCIATION ; polymorphism ; POLYMORPHISMS ; ALPHA ; STAGE ; LESIONS ; CIGARETTE-SMOKING ; risk factors ; smoking ; RATES ; PREVALENCE ; HELICOBACTER-PYLORI ; PRECANCEROUS LESIONS ; signaling ; CYTOKINE ; REGRESSION ; INCREASE ; SINGLE NUCLEOTIDE POLYMORPHISMS ; INTERVAL ; HELICOBACTER-PYLORI INFECTION ; ROLES ; INCREASED RISK ; odds ratio ; RISK-FACTOR ; Helicobacter pylori ; intestinal metaplasia ; stomach cancer ; ENVIRONMENTAL-FACTORS ; premalignant lesions ; anti-inflammatory cytokines ; IL-10 ; INTERLEUKIN-4 RECEPTOR GENE
    Abstract: Objectives The aim of the study was to assess the effects of genetic polymorphisms in anti-inflammatory mediators, i.e., IL10, IL4 and IL4R on the prevalence of gastric precancerous lesions and their interactions with other environmental factors. Methods The study population consisted of 2,033 Venezuelan subjects known to have extremely high Helicobacter Pylori (HP) infection rates. The odds ratios (OR) and 95% confidence intervals (CI) associated with these polymorphisms were estimated by multinominal logistic regression models for gastric precursor lesions. Results We found a 60% increase in risk of intestinal metaplasia (IM) and dysplasia combined (OR 1.62, 95% CI: 1.10-2.38) among the carriers of the IL10-1082 low activity allele. This increased risk was more pronounced for dysplasia than for IM. On the other hand, homozygotes with the low activity allele of the A398G polymorphism in the IL4R gene had a modest increase in risk of atrophic gastritis (OR = 1.52, 95% CI: 1.05-2.21), compared with homozygotes of the high activity allele. There were no statistically significant synergetic interactions between these polymorphisms and environmental risk factors (low fruit intake, high starchy vegetable intake and cigarette smoking) for these lesions. Conclusion While the results of the present study suggest roles of genetic variability in these anti-inflammatory mediators in different stages of gastric carcinogenesis, there is high likelihood that they were chance findings due to multiple comparisons
    Type of Publication: Journal article published
    PubMed ID: 17006724
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: BLOOD ; Germany ; HEPATOCELLULAR-CARCINOMA ; GENOME ; LINES ; DNA ; CELL-LINES ; polymorphism ; POLYMORPHISMS ; single nucleotide polymorphism ; AMPLIFICATION ; ASSAY ; PLASMA ; SNP ; LINE ; PCR ; cell lines ; STANDARD ; SERUM ; SINGLE ; RE ; SINGLE NUCLEOTIDE POLYMORPHISMS ; RESOURCE ; EXTRACTION ; PRIMER ; LEVEL ; ASSAYS ; RESOURCES ; MULTIPLE DISPLACEMENT AMPLIFICATION ; ROLLING-CIRCLE AMPLIFICATION
    Abstract: While DNA of good quality and sufficient amount can be obtained easily from whole blood, buccal swabs, surgical specimens, or cell lines, these DNA-rich sources are not always available. This is particularly the case in studies for which biological specimens were collected when genotyping assays were not widely available. In those studies, serum or plasma is often the only source of DNA. Newly developed whole genome amplification (WGA) methods, based on phi 29 polymerase, may play a significant role in recovering DNA in such instances. We tested a total of 528 plasma samples kept in storage at -40 degrees C for approximately 10 years for 8 single nucleotide polymorphisms (SNPs) using the 5' exonuclease (TaqMan (R)) assay. These specimens yielded undetectable levels of DNA following extraction with an affinity column but produced an average 52.7 mu g (standard deviation of 31.2 mu g) of DNA when column-extracted DNA was used as a template for WGA. This increased the genotyping success rate from 54% to 93%. There were only 3 disagreements out of 364 paired gehotyping results for pre- and post-WGA DNAs, indicating an error rate of 0.82%. These results are encouraging for expanding the use of poor DNA resources in genotyping studies
    Type of Publication: Journal article published
    PubMed ID: 16235563
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: CANCER ; POPULATION ; RISK ; GENE ; GENES ; GENETIC POLYMORPHISMS ; ASSOCIATION ; polymorphism ; POLYMORPHISMS ; MUTATIONS ; MYOCARDIAL-INFARCTION ; PREVALENCE ; ULCERATIVE-COLITIS ; CROHNS-DISEASE ; INFLAMMATORY-BOWEL-DISEASE ; ASSOCIATIONS ; PROMOTER POLYMORPHISM ; development ; CD14 GENE ; HELICOBACTER-PYLORI INFECTION ; GENOTYPE ; lipopolysaccharide ; Helicobacter pylori ; stomach cancer ; premalignant lesions ; ASP299GLY POLYMORPHISM ; RECEPTOR-4 GENE
    Abstract: As Helicobacter pylori (HP) is a Gram-negative bacterium, we investigated the associations between several functional polymorphisms in genes involved in lipopolysaccharide (LPS) signaling and the prevalence of various stages of gastric premalignant lesions in a Venezuelan population. The two NOD2 polymorphisms, del3020insC and Gly908Arg, were too infrequent to study their associations with gastric lesions. The risk of intestinal metaplasia (IM) was significantly increased among subjects with the CD14 T-260 allele compared to those without this allele. A similar, but nonsignificant increase in risk for dysplasia was observed among homozygotes of this allele. There was no association between TLR4 Asp299Gly polymorphism and any type of lesions, except for a slight nonsignificant increase in risk of IM associated with the AA genotype among subjects with a higher histological HP score. These results suggest that genetic polymorphisms in HP LPS signaling may be implicated in the development of intermediate stages of gastric premalignant lesions
    Type of Publication: Journal article published
    PubMed ID: 17171451
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...