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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters B 260 (1991), S. 6-10 
    ISSN: 0370-2693
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0711
    Keywords: Follicular fluid ; Oocyte ; Meiosis ; Progesterone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We studied the effect of low molecular weight fractions of human follicular fluid (FF1) on rat oocyte meiosis and progesterone secretion by the granulosa cells. Steroid-free FF1 extracts were filtered through Sephadex G50 gel. One highly retarded fraction was obtained (GF-3), which was either used for testing or further purified by filtration on Sephadex G10, the G10-3 fraction of which was used for experiments. The GF-3 and G10-3 fractions of FF1 inhibited (in a dose-dependent and reversible manner) meiosis of isolated rat oocytes during a 4-h culture. Similarly treated fractions of serum had no effect. The inhibition was not abolished by ether extraction, trypsin treatment, heating to 56° C for 1 h or boiling for 5 min, whereas heating to 105° C for 18 h decreased the effect. Gonadotropin-stimulated progesterone secretion by cumulus and mural granulosa cells was also dose-dependently and reversibly inhibited by the GF-3 fractions. Our results confirm those obtained in earlier studies on porcine oocyte maturation inhibitor (OMI).
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  • 3
    ISSN: 1420-908X
    Keywords: Key words: CD9 — Cell-interaction — Flow cytometry — Eosinophils — Platelets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective and Design: The redistribution of CD9 in peripheral blood eosinophils was investigated with respect to the interaction with platelets during in vitro activation, and whether this interaction exerts influence on eosinophil adhesion properties.¶Materials and Methods: Flow cytometry was used to investigate the CD9 expression in purified eosinophils or eosinophils from different whole blood preparations, with or without platelets present. To confirm an eosinophil/platelet interaction fluorescence microscopy was used, and to demonstrate release/shedding of CD9 molecules a biosensor technique was performed.¶Results: Our results show that both intracellular and surface expression of CD9 decrease upon in vitro activation in the absence of platelets, a phenomenon probably caused by release/shedding of soluble forms of CD9 and not due to intracellular degradation. Increased expression of CD9 on eosinophils, stimulated in the presence of platelets, is partly a result of interacting platelets, judged by the increase in platelet specific marker CD61. In our adhesion assay a significant increase in eosinophil adhesion properties to fibronectin was obtained when eosinophils were PMA stimulated and interacting with platelets, as compared to activated eosinophils without platelets.¶Conclusions: Our findings, that CD9 expression on eosinophils is dynamically regulated, support our previous suggestion that CD9 may be a useful activity marker and that platelet interaction acts on eosinophil adhesion.
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  • 4
    ISSN: 1612-4766
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Zusammenfassung Der KiefernholznematodeBursaphelenchus xylophilus (Steiner undBuhrer) Nickle ist wiederholt in Finnland und Schweden in Importen von Koniferenhackschnitzeln aus Nordamerika gefunden worden. Diese Funde waren der Anlaß zu Einfuhrbeschränkungen und Untersuchungen über Verbreitung und Biologie derBursaphelenchus-Arten. Das Vorkommen von Nematoden dieser Gattung in Schweden wurde inventiert. Es ist bekannt, daß der Kiefernholznematode von Cerambyciden der GattungMonochamus auf neue Wirstbäume und Brutholz überführt wird. Ausgehend von dem Vorkommen der vermuteten Vektoren wurden im Frühjahr 1988 für die Nematoden-Inventierung in Süd- und Mittelschweden Proben von Kiefernholz mit Befall vonMonochamus-Arten eingesammelt. Aus diesem Holz schlüpften 59 Exemplare vonM. sutor L. und 2M. galloprovincialis Ol. In 14 dieserM. sutor von 6 Standorten und in beidenM. galloprovincialis wurden Nematoden der GattungBursaphelenchus gefunden, die der ArtB. mucronatus Mamiya und Enda ähnlich sind. Dies sind die ersten Funde von Nematoden desB. mucronatus-Typs inM. galloprovincialis und das erste Mal, daß über das Vorkommen von solchen Nematoden inM. sutor in Europa berichtet wird.
    Notes: Abstract A national research program was initiated in 1988 in Sweden to provide more information on the distribution and bionomics ofBursaphelenchus species. As a result of the intimate association known to occur between the pinewood nematodeB. xylophilus (Steiner andBuhrer) Nickle and its insect vectors a survey was directed to Scots pine wood (Pinus sylvestris L.) in whichMonochamus species were breeding. A total of 59M. sutor L. and twoM. galloprovincialis Ol. emerged from the collected material. Fourteen specimens of theM. sutor and bothM. galloprovincialis specimens containedBursaphelenchus nematodes which resembled the speciesB. mucronatus Mamiya andEnda. To our knowledge this is the first record of theB. mucronatus-type nematodes inM. galloprovincialis and the first finding of such nematodes inM. sutor in Europe.
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  • 5
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: specificity of IgE binding to a human basophil-like cell line (KU812) was studied by flow cytometry. Four IgE myeloma proteins, representing both light-chain types, one chimeric IgE protein, and polyclonal serum IgE blocked the direct binding of FITC-labeled IgE(DES) myeloma protein to KU812 cells in a dose-dependent and nearly equimolar way. Although not as efficiently as human IgE (from five to eight times less on a molar basis), both rat and mouse IgE blocked IgE(DES)-FITC binding to KU812 cells. In sharp contrast, all four human IgG subclasses, both IgA subclasses, and IgM myeloma proteins, as well as monomeric and heat-aggregated polyclonal human IgG, were unable to block significantly IgE(DES)-FITC binding to KU812 cells (≤0.5/0 on a molar basis). The cytophilic epitope on IgE was heat-susceptible (56 °h), lost after reduction alkylation, and resident in the papain-derived Fcɛ -fragment, but not in the papain-derived F(ab') 2ɛ.- and Fcɛ-fragments nor in the pepsin-derived F(ab')2ɛ- and Fcɛ.'-fragments. Washing and displacement experiments indicated that a major part of IgE reacted with high affinity to KU812 cells. The results indicate that the binding of IgE to KU812 cells is highly specific and involves the classical high-affinity FcɛRI-receptor. Although the density of receptors is low, this human cell line offers a unique model to study IgE/FcɛRI interactions.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 49 (1994), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The prevalence and specificity of naturally occurring human IgA anti-IgE autoantibodies (a-E Ab) were studied by ELISA with anti-IgA monoclonal antibodies (mAb) and a purified myeloma IgE as solid-phase protein, i.e., IgE-DES(κ). Such detected IgA a-E Ab were common among adults, and significantly increased geometric means (GM) were found in patients with atopy (P= 0.006; n= 41; GM = 79.3 arbitrary units (AU)/ml) and filariasis (P= 0.02; n= 41; GM = 75.9 AU/ml), as compared with nonatopic controls (n= 42; GM = 48.8 AU/ml). No such difference was observed between age-matched nonatopic (n= 22; GM = 36.7 AU/ml) and atopic (n= 22; GM = 38.6 AU/ml) children. Children had significantly (P= 0.001) lower IgA a-E Ab concentrations than adults, probably as a result of age, because IgA a-E Ab concentrations and age of children were significantly correlated (n= 44; P〈0.05; rs= 0.30). IgA a-E Ab concentrations were very low in cord serum (n= 32; median 〈0.1 AU/ml). Sex did not influence IgA a-E Ab concentrations in any study group. The specificity of IgA a-E Ab in nine sera was studied by ELISA inhibition assay using IgE-DES myeloma as solid-phase protein and inhibitory proteins of the IgG, IgM, IgD, and IgE classes, including five different IgE myeloma proteins, as well as three enzymatic fragments of IgE-DES. The inhibitions indicated that all IgA a-E Ab tested reacted in a low-affinity reaction with determinants restricted to IgE-DES, i.e., the solid-phase protein. These epitopes were heat-resistant (2 h; 56°C) and located in the Fabɛ-DES fragment. No isotype-specific IgA a-E Ab were found because none of the four other IgE proteins were inhibitory. Subclass typing indicated that most IgA a-E Ab belonged to the IgA 1 subclass. It is unlikely, for reasons of restricted specificity, low affinity, and common prevalence, that such IgA 1 a-E Ab are connected with IgE-mediated disorders. The study also raises questions on the definition of anti-IgE antibodies.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 43 (1988), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cord serum IgE was assayed by particle counting immunoassay (PACIA) in an unselected series of European newborns (n = 190; geom mean = 0.37 IU/ml) and a cutoff limit established (≥ 1.20 IU/ml) for prediction of atopy. At control follow-up by questionnaire 18 months after birth, 38 infants (20.0%) had developed definite (9.5%) or probable (10.5%) atopy with a significant predominance of boys (P 〈 0.03). Infants with a positive immediate family history (IFH) had a higher risk of developing atopy (P 〈 0.0025) and also had a higher incidence of elevated cord IgE (P 〈 0.02) than infants with a negative IFH. Maternal atopy influenced cord IgE levels significantly (P 〈 0.00005), whereas paternal atopy did not (P= 0.23). No fetal IgE antibodies against five common allergens could be demonstrated in 36 cord sera tested. Breast-feeding for 3 months was not sufficient to prevent atopic symptoms. The predictive value of cord IgE was high since 26 of 36 newborns (positive predictive value = 72.2 %) with elevated cord IgE had developed atopic symptoms before follow-up. Of the 38 infants who developed atopic symptoms, 26 had elevated cord IgE (sensitivity = 68.4%) compared to only 10 (6.6%) of the 152 atopy-free infants (P 〈 0.00005). The data indicate that elevated cord IgE as determined by PACIA is a good predictor of early-onset atopy, better than family history (P 〈 0.008), and that primarily maternal atopy seems to affect fetal IgE synthesis.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 47 (1992), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Comparison was made of IgE and IgA levels in aspirated and gravity-collected cord blood (CB) from the umbilical vein and in capillary blood samples collected on the 4-5th day of life among 21 infants with atopic heredity. The IgA levels, but not the IgE levels, were significantly (p〈 0.001) lower at days 4-5 of life than at delivery. Further, there were significantly (p〈0.05) more infants with decreasing IgA levels (20/21; 95%) than IgE levels (9/15; 60% of those with detectable IgE, i.e., ≥ 0.125 kU/1). These observations, together with the highly significant correlation observed between IgE in aspirated CB samples and at 4–5 days of age, suggest active IgE synthesis during the prenatal and postnatal periods. Contamination of CB with maternal blood, defined as an increased CBIgA level (≥ 14.1 μg/ml), was found in 3 (14%) CB samples, all of which were gravity-collected. Of 4 CB samples (gravity-collected) with elevated IgE (i.e., ≥ 0.9kU/l), 2 had suspected maternal contamination. Therefore, aspiration of CB or capillary collection at 4-5 days of age should be preferred for allergy prediction. If gravity collection is used, contamination should be investigated by determining IgA in all CB samples with IgE concentrations exceeding the cut-off point.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 41 (1986), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Using monoclonal anti-human IgE antibodies recognizing the Dɛ1 or Dɛ2 epitope, we have developed a sandwich radioimmunoassay (RIA) to determine IgE in human cell cultures. With the help of this assay, various methods of measuring an actual de novo IgE synthesis were compared. It was necessary to substract preformed IgE from released IgE in the culture supernatant and the IgE associated to the cultured cells, in order to determine a net IgE synthesis which would reflect de novo synthesized IgE. Using this differential in vitro IgE assessment, no net IgE synthesis could be demonstrated in culture conditions which lead to strong IgG synthesis. Actual net in vitro IgE antibody production was only found in approximatively 30% of cell cultures from atopic donors. This spontaneous IgE synthesis did not correlate to the serum IgE levels of the patients. However, correlations were found between serum IgE levels and amount of preformed, released or cell-associated IgE of the cultures.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 41 (1986), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: IgG subclass levels in cord serum of unselected European newborns were studied in relation to parental smoking. Cord IgG3 was significantly (P 〈 0.01) higher in newborns of smoking mothers (n= 18; geom.mean = 87.0%) compared to those of non-smoking mothers (n= 66; geom.mean = 61.0%). Maternal smoking did not influence (P 〉 0.10) cord IgG2 or cord IgG4. Paternal smoking did not affect any of the three immunoglobulins in cord serum (P 〉 0.10). These data suggest that tobacco smoking affects IgG3 synthesis in some direct or indirect way.
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