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    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Ex-Vivo signal Transduction Studies in Chronic Lymphocytic Leukemia -- Ex vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells -- Culture and Harvest of CpG-stimulated Blood and Bone Marrow in Chronic Lymphocytic Leukemia -- Analysis of Common Abnormalities seen in Chronic Lymphocytic Leukemia using Fluorescence in situ Hybridization -- Immunoglobulin Gene Analysis in Chronic Lymphocytic Leukemia -- Detection and Functional Analysis of TP53 Mutations in CLL -- The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-mutant CLL -- Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and its Clinical Applications -- In vitro Assay to Study CLL and Monocyte Interactions -- Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells -- Measurement of Leukemic B-cell Growth Kinetics in Patients with Chronic Lymphocytic Leukemia -- Assays on DNA Damage and Repair in CLL -- Method for Generating a Patient-Derived Xenograft Model of CLL -- Immunological Methods to Study Monoclonal Antibody Activity in Chronic Lymphocytic Leukemia -- CRISPR/Cas9-based Gene Dropout Screens -- Gene Disruption using CRISPR-Cas9 Technology -- Minimal Residual Disease Quantification in Chronic Lymphocytic Leukemia: Clinical Significance and Flow Cytometric Methods -- Rare Variant Quantitation Using Droplet Digital PCR -- Methods for Measuring ctDNA in Lymphomas -- Measurement of miRNAs in Chronic Lymphocytic Leukemia Patient Samples by Quantitative Reverse Transcription PCR -- Bioinformatics Analysis of Whole Exome Sequencing Data -- Genome Sequencing and Analysis Methods in Chronic Lymphocytic Leukemia -- Characterization of Somatically-acquired Copy Number Alterations in Chronic Lymphocytic Leukaemia using Shallow Whole Genome Sequencing -- High-throughput Sequencing of the T Cell Receptor Beta Chain Gene Repertoire in Chronic Lymphocytic Leukemia
    Abstract: This book aims to provide scientists with tools and well-researched protocols to enable their research and to facilitate further progress in this common leukemia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chronic Lymphocytic Leukemia: Methods and Protocols aims to accelerate research on chronic lymphocytic leukemia and further improvements in patient outcomes
    Pages: XII, 373 p. 44 illus., 37 illus. in color. : online resource.
    ISBN: 9781493988761
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