Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1432-0517
    Keywords: Key words Traceability ; Coulometry ; Uncertainty ; Primary methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract  The possible approaches to realising a link to the SI system and the status of primary direct methods in the traceability chain of chemical measurements are discussed. Some results obtained with the new coulometric standard system are presented.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-0568
    Keywords: Perinatal ; Neonatal ; Adult ; Type II cell ; Sheep lung development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The quantitative morphologic changes in alveolar type II cells during the perinatal period were characterized morphometrically in the lungs of fetal lambs at 132, 138, and 147 days gestational age (DGA) and in newborns at 2 days postnatal age (2 DPN). Ultrastructural features were compared with those of type II cells of ewes 365 days old. Lamellar body profile number per type II cell profile was highest at term (147 DGA) and 2 DPN. In adults, the number of lamellar body profiles and volume density of lamellar bodies were equal to those of the 132 DGA fetus. Multivesicular bodies were most common at 138 DGA and in adults. The volume density of cytoplasmic glycogen fell dramatically during the latter part of gestation. The volume density of many cellular organelles increased to the level observed in adults by term (147 DGA). Subcellular composition of type II cells of adult sheep differs from that reported for adult rats chiefly by the volume density of lamellar material within the cytoplasm. Plate-like or globe-like inclusions were present only in the type II cells of adults. Cytoplasmic extensions of the type II cell crossing the basal lamina were most abundant in the 132 and 138 DGA fetal sheep. Cytoplasmic extensions were rare in adults. We conclude that morphologic changes of the alveolar type II cell associated with gestational age follow a species-specific time course. In the sheep, this occurs during the later part of gestation and extends into the neonatal period. Morphologic and morphometric changes appear to correspond with cellular interactions between alveolar type II cells and mesenchymal cells of the interstitium.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 46 (1990), S. 561-562 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 46 (1990), S. 2041-2043 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 47 (1991), S. 1204-1206 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: We examined histochemically (light microscopy-LM) and cytochemically (electron microscopy-EM) the secretory epithelial cells in the tracheobronchial mucosa of sheep. Six morphologically distinct, granule-containing cells have been described, on the basis of their morphology and airway distribution: four mucous (M1-M4), serous (SC), and Clara (CC). Stereological and morphometric data indicated that M3, M4, SC, and CC were distinctly different from each other and from M1 and M2 cells. Mucous cells M1 and M2 differed in granule morphology. Samples of tracheas, sixth-generation bronchi, distal bronchi, and terminal bronchioles of 18 adult sheep were examined. At the LM level, methacrylate sections were reacted with an alcian blue (pH 2.5), periodic acid Schiff (PAS) sequence to differentiate neutral from acidic glycoconjugates (GC), and a high-iron diamine (HID), alcian blue sequence to differentiate sulfated from nonsulfated (sialated) GC. At the EM level the periodic acid-thiocarbohydrazide localized hexose-rich, neutral GC. Dialyzed iron (DI) and high-iron diamine localized carboxylated and sulfated GC, respectively. Granules of all but Clara cells were PAS-positive. All mucous cells contained acidic groups, but only M1 and M4 cells had LM-detectable sulfated GC. At the ultrastructural level, minimal but discernible HID and LID reaction product was observed on granule profiles of M2, M3, and SC, indicating acidic and sulfated GC not detected at the LM level. Histochemically, the sheep tracheobronchial epithelium was more similar to that of humans than some other examined mammalian species.
    Additional Material: 40 Ill.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Conventional histochemical characterization of the mucus secretory apparatus is often difficult to reconcile with the biochemical analysis of respiratory secretions. This study was designed to examine the secretory glycoconjugates in airways using lectins with biochemically defined affinities for main sugar residues of mucus. We used five biotinylated lectins - DBA (Dolichos biflorus) and SBA (Glycine max) for N-acetyl galactosamine (gaINAc), BSAI (Bandeiraea simplicifolia) and PNA (Arachis hypogea) for galactose (gal), UEA I (Ulex europeus) - for detection of fucose (fuc) in HgCl2 -fixed, paraffin-embedded, serially sectioned trachea, lobar and segmental bronchi and bronchioles of nine sheep. Lectins selectively localized the carbohydrate residues in lumminal secretions, on epithelial cell surfaces, and in secretory cells. Inp proximal airways, the major carbohydrate residues in luminal secretions, cell surfaces, goblet cells, and glands were fuc and gal-NAc. PNA reacted mainly with apical granules of 〈 10% of goblet cells, and gal residues were only detected in some of the mucous cells and on basolateral cell surfaces. Distal airways contained sparse secretion in the lumen, mucous cells containde weakly reactive fuc and gal-NAc, and the epithelial surfaces of Clara cells contained gal. Sugars abundant in the airway secretions were also the major component of cells in glands. We conclude that there is a correlation between specific sugar residues in secretory cells, glycocalyx, and luminal secretions in proximal and distal airways. This suggests that lectins may be used to obtain information about airway secretory cell composition from respiratory secretions.
    Additional Material: 24 Ill.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The surface characteristics of the intrapulmonary airways and alveoli of the lungs from 12 young adult cattle examined by the scanning electron microscope are described. When compared with similar studies of the lungs of various other mammalian species, the bovine lung gneerally resmebles that of other mammals except for several important features: (1) Alveolar pores of Kohn are small and extremely rare; (2) Alveolar macrophages are seldom seen in alveolar air spaces; (3) Interlobular septa completely separate the lung into distinct lobules.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Glutaraldehyde-infused tracheas and airways of five castrated sheep were microdissected following the axial airway of the left cranial and caudal lobes. Airway branches were assigned binary numbers indicating their specific location in the tracheobronchial tree. Samples of known airway generation were resin embedded and examined by light-microscopy. Based on differences in cell morphology, staining properties, and distribution, eight major cell groups were recognized and quantified: four mucous cell categories (M1, M2, M3, and M4), ciliated, basal, Clara, and serous cells. The last cell category was restricted to submucosal glands. Tracheal epithelium had the most cells per unit length, primarily due to large numbers of basal cells. Basal cells are found in the epithelium of airways without cartilage or glands. The total mucous cell population (M1, M2, and M3) in proximal airways was relatively constant. M4 mucous cells were present in glands of proximal airways and in the epithelial lining of the airways without glands. The most distal airways were lined by Clara and ciliated cells. A small number of the most proximal noncartilaginous airways had mucous (M1, M2, M3, and M4), basal, and Clara cells sharing the epithelial lining. We conclude that in the sheep lung: (1) epithelial cell distribution does not correlate with airway wall components; (2) more than one type of secretory epithelial cell can share the lining of the same airway; and (3) Clara cell distribution is based on airway generation and proximity to alveoli.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In a light microscopic study we have described the morphology and distribution of six distinct, granule-containing cells in the tracheobronchial epithelium of sheep lung. We designed the present study to determine qualitatively and quantitatively whether these six cell types differ in ultrastructural morphology. Cell height varied from 30.6 μm for mucous cell M1 to 9.6 μm for Clara cells. Cell width varied from 21.2 μm for M1 to 9.3 μm for Clara cells. Nuclear dimensions ranged from 7.5 μm in M3 to 4.0 μm in M1 and M2. Mucous cell M1 had electron-dense granules (1.5 μm in diameter); M2, electron-lucent granules (1.6 μm); M3, nucleated electron-lucent granules (0.51 μm); M4, cored granules (1.1 μm); serous (SC) and Clara cells (CC), electronopaque granules (0.58 μm and 0.37 μm). The volume fraction of the cell occupied by granules was 63% in M1 and M2, M4 39%, SC 23%, CC 5%, and M3 4.5%. Smooth endoplasmic reticulum was observed only in M3 (33.8%) and CC (49%). Granular endoplasmic reticulum (GER) was most abundant in SC (21%) and least plentiful in M4 (2.2%). We conclude that mucous cells M3 and M4 and serous and Clara cells differ from each other and from M1 and M2 cells. Mucous cells M1 and M2 differ from each other only in amount of GER and secretory granule appearance.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...