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  • 1
    Keywords: Medicine ; Oncology ; Biomedicine ; Cancer Research ; Oncology ; Springer eBooks
    Description / Table of Contents: Novel Insights/translational Implication from the Emerging Biology of Melanoma -- Emerging Clinical Issues in Melanoma in the Molecularly Targeted Era -- Integrating Molecular Biomarkers Into Current Clinical Management In Melanoma -- Advances in Adjuvant Therapy: Potential for Prognostic and Predictive Biomarkers -- Immunologic Monitoring of Cancer Vaccine Trials Using the ELISPOT Assay -- Markers for Anti-Cytotoxic T-lymphocyte Antigen 4 (CTLA-4) Therapy in Melanoma -- Marker Utility for Combination Therapy -- Assaying for BRAF V600E in Tissue and Blood in Melanoma -- Selecting Patients for KIT Inhibition in Melanoma -- Detecting Mechanisms of Acquired BRAF inhibitor Resistance in Melanoma -- Current Status of Diagnostic and Prognostic Markers in Melanoma -- Chromosomal Copy Number Analysis in Melanoma Diagnostics -- Construction and Analysis of Multi-Parameter Prognostic Models for Melanoma Outcome -- Immunohistochemical Diagnostic and Prognostic Markers for Melanoma -- Lymphatic Invasion as a Prognostic Biomarker in Primary Cutaneous Melanoma -- Tumor Infiltrating Lymphocytes and Their Significance in Melanoma Prognosis -- Pathological Staging of Melanoma -- Genotyping of Human Leukocyte Antigen (HLA) Ancestral Haplotypes as Prognostic Marker in Cancer Using PCR Analysis -- B7-H Abnormalities in Melanoma and Clinical Relevance -- Melanoma Susceptibility Genes and Risk Assessment -- Clinical, Pathologic, and Imaging Features, and Biological Markers of Uveal Melanoma -- A Prognostic Test to Predict Risk of Metastasis in Uveal Melanoma Based on a 15-Gene Expression Profile -- Molecular Karyotyping for Detection of Prognostic Markers in Fine Needle Aspirate Biopsy Samples of Uveal Melanoma -- ERBB4 Mutation Analysis: Emerging Molecular Target for Melanoma Treatment -- Epigenetic Markers of Prognosis in Melanoma -- Isolation of Melanoma Cell Subpopulations Using Negative Selection -- Circulating Tumor Cells as Prognostic Biomarkers in Cutaneous Melanoma Patients -- Detection of Chondroitin Sulfate Proteoglycan 4 (CSPG4) in Melanoma -- Targeting Damage Associated Molecular Pattern Molecules (DAMPs) and DAMP Receptors in Melanoma -- The Clinical Use of PET/CT in the Evaluation of Melanoma -- Immune System Functional Pathway Analysis Using Single Cell Network Profiling (SCNP): A Novel Tool in Cancer Immunotherapy -- Quantitative and Spatial Image Analysis of Tumor and Draining Lymph Nodes Using Immunohistochemistry and High Resolution Multispectral Imaging to Predict Metastasis -- COLD-PCR Enriches Low-Level Variant DNA Sequences and Increases the Sensitivity of Genetic Testing -- Isolation of Circulating MicroRNAs from Microvesicles found in Human Plasma -- Detection of Circulating Tumor Cells by Photoacustic Flowmetry -- Statistical Design and Evaluation of Biomarker Studies -- Tissue Resources for Clinical Use and Marker Studies in Melanoma
    Abstract: In Molecular Diagnostics for Melanoma: Methods and Protocols, expert researchers and clinicians in the field of melanoma provide updated information on biomarkers and assays for diagnosis, prognosis, and assays predicting response to treatment for routine testing. The focus of the volume is on biomarkers with established clinical validity rather than those on early discovery stage.℗ With additional in-depth discussion of the molecular biology and pathology of melanoma, treatment options in adjuvant and metastatic setting, and implications of biomarker testing for clinical management of melanoma patients. ℗ Written in the highly successful Methods in Molecular Biology series format, chapters include extensive introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Comprehensive and practical, Molecular Diagnostics for Melanoma: Methods and Protocols seeks to provide both clinicians and scientists with technical information and extensive background information on the wide ranging approaches available in the field of diagnostics of melanoma
    Pages: XXIII, 712 p. 110 illus., 94 illus. in color. : online resource.
    ISBN: 9781627037273
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  • 2
    Unknown
    New York, NY : Springer Science+Business Media
    Keywords: Medicine ; Oncology ; Biomedicine ; Cancer Research ; Springer eBooks
    Pages: : digital
    ISBN: 9781441972194
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  • 3
    ISSN: 1534-4681
    Keywords: Melanoma ; Dendritic cells ; Melanoma-associated antigens ; MART-1 ; Recombinant viral vectors ; Poxviruses ; CTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Dendritic cells (DC) are potent professional antigen-presenting cells that can activate naive T lymphocytes and initiate cellular immune responses. As adjuvants, DC may be useful for enhancing immunogenicity and mediating tumor regression. Endogenous expression of antigen by DC could offer the potential advantage of allowing prolonged constitutive presentation of endogenously processed epitopes and exploitation of multiple restriction elements for the presentation of the same antigen. Methods: DC were prepared from the peripheral blood of HLA A*0201 patients with metastatic melanoma in the presence of IL-4 (1000 IU/mL) and GMCSF (1000 IU/mL). Recombinant vaccinia and fowlpox viruses encoding the hMART-1 gene were constructed and used to infect DC. The efficiency of infection and expression of the MART-1 antigen were assessed by immunohistochemistry and intracellular FACS analyses. Cytotoxic lymphocytes (CTL) were generated by the stimulation of CD8+ T cells, with DC expressing the recombinant gene. Reactivity of the CTL was determined at weeks 1 and 2 by the amount of IFN-γ released. Results: DC were infected with recombinant poxviruses and demonstrated specific melanoma antigen expression by immunohistochemistry, immunofluorescence, and intracellular FACS analysis. The expression by DC of MART-1 MAA after viral infection was sufficient to generate CD8+ T lymphocytes that recognized naturally processed epitopes on tumor cells in 10 of 11 patients. Conclusions: Human DC are receptive to infection by recombinant poxviruses encoding MAA genes and are capable of efficiently processing and presenting these MAA to cytotoxic T cells. The potential advantage of this approach is the ability to present specific antigen independent of the identification of the epitope or the MHC restriction element. This strategy may be useful for the identification of relevant epitopes for a diverse number of HLA alleles and for active immunization in patients.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature medicine 10 (2004), S. 1278-1279 
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] To the editor: In the September issue of this journal, Rosenberg and colleagues published a turning-point article on the state of the art and future of cancer immunotherapy. The authors report a low tumor regression rate (2.6–3.3%) in patients (n = 1,205) with metastatic cancer who underwent ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] 'Naked' nucleic acid vaccines are potentially useful candidates for the treatment of patients with cancer, but their clinical efficacy has yet to be demonstrated. We sought to enhance the immunogenicity of a nucleic acid vaccine by making it 'self-replicating'. We accomplished this by using a ...
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The cloning of the genes encoding cancer antigens has opened new possibilities for the treatment of patients with cancer. In this study, immunodominant peptides from the gp100 melanoma-associated antigen were identified, and a synthetic peptide, designed to increase binding to HLA-A2 molecules, was ...
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature medicine 11 (2005), S. 705-708 
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Go see the human beings who are suffering, and then ask yourself, is the work I did today relevant to human suffering? Did I do something that is going to help to change somebody's life, maybe not today but sometime soon? –Christopher Reeve, accepting the American Society for Cell Biology ...
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The completion of the Human Genome Project has made possible the comprehensive analysis of gene expression, and cDNA microarrays are now being employed for expression analysis in cancer cell lines or excised surgical specimens. However, broader application of cDNA microarrays is limited by the ...
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] A widely perceived opportunity for the use of microarrays is the examination of gene expression across collections of cancer cells derived from both tumour cell lines and tumours. Primary goals of such a cataloging effort will be to determine whether there are subtypes of cancer that can be ...
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0843
    Keywords: Key words Tumor immunity ; Vaccination ; Cytotoxic T lymphocytes ; Localization ; Human leukocyte antigen tetramers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  In recent years significant progress in the understanding of the immune biology of melanoma has evolved from the identification of melanoma antigens (MAs) recognized by T cells. MAs consist of intracellular proteins that are expressed on the surface of cancer cells in association with human leukocyte antigen (HLA) class I molecules and therefore are suitable targets for cytotoxic T lymphocytes (CTLs). Several new monitoring strategies have been implemented to evaluate the status of activation and localization of vaccine-induced T cells in the peripheral circulation as well as the tumor site, including limiting dilution, in vitro sensitization, and ELISPOT. Previous studies aimed at monitoring patients receiving vaccination have utilized mainly those three methods. These methods have demonstrated that antigen-specific vaccination can elicit immune responses detectable in the peripheral blood of immunized patients. These assays, however, have been faulted by their requirement for in vitro expansion of T cells (limiting dilution or in vitro sensitization) or for limited sensitivity (ELISPOT). More recently, the use of soluble HLA/peptide complex tetramers, intracellular fluorescence-activated cell sorting (FACS) analysis, and real-time polymerase chain reaction (PCR) has been proposed for the monitoring of vaccine trials. These methods have the appeal of allowing direct enumeration of T cells specific for a particular epitope within relevant samples such as peripheral blood lymphocytes, lymph nodes, and tumors. We are evaluating whether utilizing a combination of HLA/peptide tetramer (tHLA) together with Taqman-based real-time reverse-transcription (RT)-PCR and intracellular FACS analysis could establish a direct and comprehensive strategy for the assessment of epitope-specific immune response in vivo. In conditions close to those of the tumor microenviroment or in peripheral blood lymphocytes, however, a different status of T cell activation can be expected due to a direct stimulation of T cells by tumor or antigen-presenting cells. We observed that activated T cells can easily be detected in the peripheral blood of patients who have received MA-specific vaccines. However, when T cells are stimulated with their relevant epitope, a high level of T cell receptor downregulation occurs that does not allow identification of vaccine-specific T cells directly with tHLA. Thus evaluation of epitope-specific T cells at the tumor site, where they might be exposed to stimulation by interaction with tumor cells and/or in bulk peripheral blood mononuclear cells, might be more efficiently analyzed with functional methods such as intracellular FACS and Taqman-based real time RT-PCR.
    Type of Medium: Electronic Resource
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