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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  21. Jahrestagung der Deutschen Gesellschaft für Pädiatrische Infektiologie (DGPI); 20130425-20130427; Würzburg; DOC13dgpi76 /20130328/
    Publication Date: 2013-03-29
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 2
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemistry of bromodeoxyuridine (BrdU) incorporated in DNA was performed on cryostat sections of rat liver and on isolated hepatocytes after partial hepatectomy using a two-step labeling technique. The method enabled the detection of S-phase nuclei in both tissue preparations. Quantification of the number of labeled nuclei in sections showed that the number of nuclei in S-phase increased from 0.3% in control liver to about 36% at 24 h after partial hepatectomy. The detection of BrdU in isolated hepatocytes showed the same labeling index of binuclear diploid, mononuclear tetraploid and binuclear tetraploid cells. A special role for mononuclear diploid cells in proliferation did not seem to occur.
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  • 3
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The diurnal variation of 5′-nucleotidase activity in periportal and pericentral areas of rat liver parenchyma has been determined with quantitative histochemical means. 5′-Nucleotidase activity was estimated using microdensitometry in cryostat sections after being incubated with a medium according to Wachstein and Meisel (1957). It appeared that 5′-nucleotidase activity was significantly higher in pericentral areas than in periportal areas throughout the daily cycle and showed a maximum at the end of the light period. It was concluded that 5′-nucleotidase activity may be related with the capacity to diminish messenger RNA resulting in protein breakdown.
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  • 4
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of ischemia on the stability, i.e. the permeability of the lysosomal membrane of rat liver has been studied using quantitative histochemical analysis of acid phosphatase activity. Ischemia in vitro was performed for 0–240 min at 37° C and ischemia in vivo for 60 min was followed by 1, 5, 24 and 48 h of reperfusion. Acid phosphatase activity was demonstrated in cryostat sections using naphthol AS-BI phosphoric acid as substrate and polyvinyl alcohol was added to the incubation medium to counteract diffusion phenomena. Ischemia in vitro up to 240 min did not affect the localization nor the total activity of acid phosphatase activity. After 60-min ischemia in vivo followed by 1-h reperfusion distinct areas showed decreased acid phosphatase activity. A further decrease in activity was observed after 5 h reperfusion. Final reaction product generated by acid phosphatase activity was rather diffusely distributed in border zones between normal and damaged tissue after 24 and 48 h of reperfusion following 60 min ischemia in vivo. It is concluded that not ischemia itself but rather reperfusion affects the stability of the lysosomal membrane due to the occurrence of oxygen-derived free radicals and/or imbalanced Ca2+ concentration. Restoration of the blood flow causes leakage of acid phosphatase from the lysosomes into the cytoplasm of liver parenchymal cells and from there to the blood.
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  • 5
    ISSN: 1433-044X
    Keywords: Schlüsselwörter ,“Marjolin Ulcus“• Kombinationstherapie • Fallbericht ; Key words Marjolin's ulcer • Combined therapy • Case report
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The ,,Marjolin's ulcer” is a malignant lesion which is developed especially at chronic wounds after burn trauma. The latency from primary trauma to the malignant transformation is about 30 years. The lesion is more aggressive than other squamous cell carcinomas. We report on the combined radiation and surgical therapy of an 65-year-old patient with this lesion on the right hand and regional lymph node metastases. The extensive tissue defect after wide excision was reconstructed with a reversal radial forearm flap.
    Notes: Zusammenfassung Als„Marjolin Ulcus“ bezeichnet man maligne Transformationen chronischer Wunden insbesondere nach Verbrennungstraumen. Die Latenzzeit zwischen dem Primärtrauma und der malignen Transformation beträgt etwa 30 Jahre. Das„Marjolin Ulcus“ zeichnet sich durch ein aggressiveres Verhalten gegenüber anderen Plattenepithelkarzinomen aus. In einem Fall eines 65 jährigen Patienten mit der Läsion am rechten Handrücken und regionalen Lymphknotenmetastasen berichten wir über die kombinierte Strahlen- und chirurgische Therapie. Der ausgedehnte Weichteildefekt nach entsprechend breiter lokaler Exzision wurde mit einem retrograd gestielten A. radialis-Lappen gedeckt.
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  • 6
    ISSN: 1433-0563
    Keywords: Key words Amifostine • Cytoprotection • Cisplatin • Nephrotoxicity • Bladder cancer ; Schlüsselwörter Amifostin • Zytoprotektion • Blasenkarzinom¶Cisplatin • Nephrotoxizität
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die cisplatinbasierte Polychemotherapie beim Blasenkarzinom nach dem MVEC-Schema ist zur Standardtherapie unter adjuvanter und induktiver Zielsetzung geworden. Problematisch gerade bei den älteren Patienten ist die nephrotoxische Potenz bei hoher Cisplatindosierung. Ziel unserer Untersuchung war die Beurteilung der klinischen Wirksamkeit des Zytoprotektivums Amifostin zur Prävention cisplatininduzierter Nebenwirkungen. 17 konsekutive mit pT3b-4N0–1 Urothelkarzinom der Blase wurden nach MVEC (1–6 Zyklen) polychemotherapiert; Amifostin wurde in einer Dosis von 740–910 mg/m2 30 min vor Cisplatingabe iv. appliziert. Es erfolgte die Bestimmung von Krea-Clearance, Kreatinin, Harnstoff, Elektrolyten vor und nach einem jeden MVEC-Kurs. 12 konsekutive Patienten mit pT3b-4N0–1 Urothelkarzinom der Blase erhielten MVEC (1–4 Kurse) ohne Amifostingabe und dienten als Kontrollgruppe. Es wurden keine schwerwiegenden Komplikationen unter Amifostin beobachtet, 1mal kam es zu einer reversiblen Hypokalziämie. Kreatinin und Harnstoff unterschieden sich zwischen beiden Gruppen vor und nach Therapie nicht signifikant. Die Kreatininclearance zeigte jedoch signifikante Differenzen: 125 ± 20 ml/min vor, 115 ± 25 ml/min nach Beendigung der MVEC-Therapie in der Amifostin- versus 121 ± 30 ml/min und 85 ± 20 ml/min in der Kontrollgruppe (p 〈 0,02). Bezüglich hämatotoxischer Komplikationen wurde eine signifikante Leukopenie und substitutionspflichtige Thrombozytopenie bei 29 % und 12 % der Patienten in der Amifostin- bzw. bei 67 % und 33 % in der Kontrollgruppe beobachtet. Eine hochgradige Granulozytopenie fand sich bei 6 % der Patienten unter Amifostingabe, aber bei 25 % der Patienten in der Kontrollgruppe. Es lagen keine Differenzen in der Frequenz cisplatininduzierter oto- und neurotoxischer Komplikationen zwischen beiden Gruppen vor. Durch die präventive Applikation von Amifostin können Häufigkeit und Ausprägung der cisplatinassoziierten Nephrotoxizität signifikant reduziert werden. Da dem renoprotektiven Effekt nur eine geringe Rate assoziierter Nebenwirkungen gegenübersteht, sehen wir die primäre Indikation für die präventive Amifostingabe gerade bei älteren Patienten mit bereits reduzierter Nierenfunktion.
    Notes: Summary Amifostine is an organic thiophsophate which protecvts normal cells from the effects of chemotherapy with reduced nadir and duration of cyclophosphmamid induced neutropenia, reduced cisplatin derived renal and neurological complications being described. However, no data are available for urological malignancies treated with cisplatin-based chemotherapy. Aim of the study was to assess the efficacy of pretreatment with amifostine in terms of prevention of renal, hematopoetic and neurologic toxicity. 17 patients (mean age: 62.6 [45–74]) with advanced transitional cell carcinoma of the urinary bladder received inductive or adjuvant cisplatin based chemotherapy (1–6 cycles) with a cisplatin dose of 70 mg/m2. Amifostine (740–910 mg/m2) was administered intravenously 30 minutes prior to chemotherapy. For all patients creatinine clearance, serum creatinine and electrolytes including magnesium, and blood cell count were determined prior to and after each cycle. A group of 12 patients (mean age: 61.9 [51–67]) did undergo MVEC chemotherapy (1–4 cycles) without receiving amifostine and served as control group. Amifostine was well tolerated and only 1 patient suffered from gastrointestinal discomfort, blood pressure remained unchanged in all patients. Amifostine prevented a significant reduction of creatinine clearance even in the 2 patients with known renal insufficiency: mean creatinine clearance was 125 ± 20 ml/min prior to and 115 ± 25 ml/min after chemotherapy. In the control group, however, creatinine clearance dropped from 121 ± 30 ml/min to 85 ± 20 ml/min after completion of MVEC chemotherapy. Serum creatinine levels did not increase significantly (1.1 mg/dl prior to and 1.2 mg/dl after chemotherapy), magnesium levels did not decrease significantly and normalized at the end of chemotherapy. Significant neutropenia and thrombocytopenia developed in 29 % and 12 % of the patients in the amifostine group and in 67 % and 33 % of the patients in the control group. Amifostine was shown to have a protective effect against cisplatin induced nephrotoxicity in the elderly patient undergoing systemic chemotherapy. Based on our data amifostine should be applied in the supportive management to prevent chemotherapy induced complications.
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  • 7
    ISSN: 1431-4630
    Keywords: Key words Amazon ; Solanum sessiliflorum ; Fruit ; Chemical composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Solanum sessiliflorum is a shrub native to the upper Orinoco region of the Amazon basin, previously cultivated by Indians throughout the Amazon basin because of its edible fruits, which are rarely used nowadays. Analyses of its main components, i. e. carbohydrates, minerals and trace elements, organic acids, fatty acids, free amino acids, biogenic amines and volatile compounds, are presented.
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  • 8
    ISSN: 1431-4630
    Keywords: Key words Amazon ; Caryocar villosum ; Fruit pulp ; Chemical composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract   Caryocar villosum is a fruit-bearing tree that grows in Amazonian tropical forests. Its butter-textured, yellowish fruit pulp is much liked by the inhabitants of the region. Analysis of the main ingredients, i.e. carbohydrates, minerals and trace elements and fatty acids as well as the composition of the sterol fraction, the free amino acids, the biogenic amines and volatile compounds are presented.
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  • 9
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
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  • 10
    ISSN: 1431-4630
    Keywords: Key words L-Dehydroascorbic acid ; Degradation reactions ; Transition metal catalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The initial steps of the degradation of L-de- hydroascorbic acid (L-DHA) in acidic aqueous solutions and the catalytic effect of different transition metal ions on this reaction were studied. The main product was identified as 3,6-furanosido-2,3-hexodiulosonic acid-2-hydrate (compound I) by GLC-MS and 13C-NMR, formed by lactone hydrolysis and hydration of the carbonyl group in the C-2 position of L-dehydroascorbic acid. In addition, number of other compounds were detected; they are formed from compound I by simple enolisation, lactonisation, hydration and dehydration reactions as well as by cleavage and formation of cyclic half acetal bonds. The chemical structures of these compounds were tentatively deduced by the mass spectra of their TMS derivatives and a reaction scheme for their formation is proposed. The velocity and the direction of the reactions were found to be strongly influenced by the presence of catalytic amounts of different transition metal ions. It was concluded that in acidic medium, in contrast to the situation in neutral and alkaline solutions, the opening of the lactone ring of L-DHA is, to a certain degree, a reversible reaction.
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