Description / Table of Contents:
Introduction to Mass Spectrometry-Based Proteomics -- LC-MS Spectra Processing -- Isotopic Distributions -- Retention Time Prediction and Protein Identification -- Algorithms for Database Dependent Search of MSMS Data -- Interpretation of Tandem Mass Spectra of Post-Translationally Modified Peptides -- Improving Peptide Identification Using Empirical Scoring Systems -- Methods and Algorithms for Quantitative Proteomics by Mass Spectrometry -- Computational Approaches to Selected Reaction Monitoring (SRM) Assay Design -- Feature Selection and Machine Learning with Mass Spectrometry Data -- Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data -- Permethylated N-Glycan Analysis with Mass Spectrometry -- Mass Spectrometry Methods for Studying Glycosylation in Cancer -- Proteomics Data Exchange and Storage: The Need for Common Standards and Public Repositories -- Tools for Protein Post Translational Modifications Analysis: FAK, A Case Study -- Proteomic Strategies to Characterize Signaling Pathways -- Simple Proteomics Data Analysis in the Object-Oriented PowerShell
Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably.℗ Fully revised and expanded, Mass Spectrometry Data Analysis in Proteomics, Second Edition presents expert chapters on specific MS-based methods or data analysis strategies in proteomics. The volume covers data analysis topics relevant for quantitative proteomics, post translational modification, HX-MS, glycomics, and data exchange standards, among other topics.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Updated and authoritative, Mass Spectrometry Data Analysis in Proteomics, Second Edition serves as a detailed guide for all researchers seeking to further our knowledge in the field of proteomics
X, 405 p. 93 illus., 59 illus. in color. : digital.
2nd ed. 2013.