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  • 1
    Keywords: POLYMERASE-CHAIN-REACTION ; RIBOSOMAL-RNA ; COMPLETE GENOME SEQUENCE ; PHYLOGENETIC ANALYSIS ; INFRARED-SPECTROSCOPY ; RAT-BITE FEVER ; HONGKONGENSIS SP NOV. ; FUSOBACTERIUM-NUCLEATUM ; FOURIER-TRANSFORM ; MONILIFORMIS
    Abstract: The genus Streptobacillus (S.) remained monotypic for almost 90 years until two new species were recently described. The type species, S. moniliformis, is one of the two etiological agents of rat bite fever, an under-diagnosed, worldwide occurring zoonosis. In a polyphasic approach field isolates and reference strains of S. moniliformis, S. hongkongensis, S. felis as well as divergent isolates were characterized by comparison of molecular data (n = 29) and from the majority also by their physiological as well as proteomic properties (n = 22). Based on growth-independent physiological profiling using VITEK2-compact, API ZYM and the Micronaut system fastidious growth-related difficulties could be overcome and streptobacilli could definitively be typed despite generally few differences. While differing in their isolation sites and dates, S. moniliformis isolates were found to possess almost identical spectra in matrix-assisted laser desorption ionization-time of flight mass spectrometry and Fourier transform infrared spectroscopy. Spectroscopic methods facilitated differentiation of S. moniliformis, S. hongkongensis and S. felis as well as one divergent isolate. Sequencing of 16S rRNA gene as well as functional genes groEL, recA and gyrB revealed only little intraspecific variability, but generally proved suitable for interspecies discrimination between all three taxa and two groups of divergent isolates.
    Type of Publication: Journal article published
    PubMed ID: 26252790
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  • 2
    Keywords: GENES ; RNA ; ARTHRITIS ; INFECTION ; MULTIPLE SEQUENCE ALIGNMENT ; STRAINS ; MAXIMUM-LIKELIHOOD ; RAT BITE FEVER ; TURKEYS
    Abstract: A pleomorphic, Gram-stain-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium (strain 131000547(T)) was isolated from the lungs of a cat with pneumonia. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the genus Streptobacillus with 97.6% sequence similarity to the type strain of Streptobacillus moniliformis and 94.6% to that of Streptobacillus hongkongensis. The clear differentiation of strain 131000547(T) from Streptobacillus moniliformis and Streptobacillus hongkongensis was also supported by gyrB, groEL, and recA nucleotide and amino acid sequence analysis. DNA-DNA hybridization demonstrated 〈= 19.9% (reciprocal 28.7%) DNA-DNA relatedness between strain 131000547(T) and Streptobacillus moniliformis DSM 12112(T). Physiological data confirmed the allocation of strain 131000547(T) to the family Leptotrichiaceae. Strain 131000547(T) has a unique profile of enzyme activities allowing differentiation from the most closely related species. Within the genus Streptobacillus, isolate 131000547(T) could also unambiguously be separated from Streptobacillus moniliformis and Streptobacillus hongkongensis by both matrix-assisted laser desorption ionization time-of-flight mass spectrometry and Fourier transform-infrared spectroscopy. On the basis of these data, a novel species of the genus Streptobacillus, Streptobacillus felis sp. nov., is proposed with the type strain 131000547(T) (=DSM 29248(T)=CCUG 66203(T)=CCM 8542(T)). Emended descriptions of the genus Streptobacillus and of Streptobacillus moniliformis are also given.
    Type of Publication: Journal article published
    PubMed ID: 25858245
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  • 3
    Abstract: A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase- negative, non-spore-forming, non-motile bacterium was 1979 originally isolated from the heart of an Australian spinifex hopping mouse with septicaemia and stored as Streptobacillus moniliformis in the strain collections of Animal Health Laboratory, South Perth, Western Australia (AHL 370-1) as well as under CCUG 12425. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the genus Streptobacillus with 99.4% sequence similarity to Streptobacillus moniliformis, 95.6% sequence similarity to Streptobacillus hongkongensis and 99.0% sequence similarity to Streptobacillus felis. The clear differentiation of strain AHL 370-1T from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB, groEL, and recA nucleotide and amino acid sequence analysis. Average nucleotide identity revealed 87.16% (reciprocal 87.16%) DNA-DNA relatedness between strain AHL 370-1T and Streptobacillus moniliformis DSM 12112T.Physiological data confirmed the allocation of strain AHL 370-1T to the family Leptotrichiaceae considering very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus, isolate AHL 370-1T could also unambiguously be separated from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2, KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1T. On the basis of these data we propose the novel species Streptobacillus notomytis sp. nov. with the type strain AHL 370-1T (= CCUG 12425T = DSM 100026T = CCM 8593T = EF 12425).
    Type of Publication: Journal article published
    PubMed ID: 26438009
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