Post-translational modification of proteins
Description / Table of Contents:
Electrophoretic Mobility Shift Assay Analysis of NF-̐±B DNA Binding -- Detection of IkB Degradation Dynamics and IkB-a Ubiquitination -- Measurement of NF-ÎðB Transcriptional Activity and Identification of NF-ÎðB cis-regulatory Elements using Luciferase Assays -- Assessing Sites of NF-ÎðB DNA Binding using Chromatin Immunoprecipitation -- IKK Kinase Assay for Assessment of Canonical NF-kB Activation in Neurons -- Analysis of Epidermal Growth Factor-induced NF-kB Signaling -- Methods to Assess the Activation of the℗ Alternative (non-canonical)℗ NF-kB Pathway by Non-death TNF Receptors -- Systematic Detection of Non-canonical NF-ÎðB Activation -- Non-canonical NF-ÎðB activation and SDF-1 expression in Human Endothelial Cells -- Stable Reconstitution of IKK-deficient Mouse Embryonic Fibroblasts -- Dissecting NF-ÎðB Signaling Induced by Genotoxic Agents Via Genetic Complementation of NEMO-deficient 1.3E2 Cells -- Visualizing TCR induced POLKADOTS Formation and NF-ÎðB Activation in the D10 T Cell Clone and Mouse Primary Effector T cells -- Detection of Recombinant and Cellular MALT1 Paracaspase Activity -- TRAF Protein Function In Non-Canonical NF-ÎðB Signaling -- Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-kB Signaling -- Elucidating Dynamic Protein-protein Interactions and Ubiquitination in NF-kB Signaling Pathways -- Immunoblot Analysis of Linear Polyubiquitination of NEMO -- In vitro Detection of NEMO-Ubiquitin Binding using DELFIA and Microscale Thermophoresis Assays -- Use of Fluorescence Spectroscopy For Quantitative Investigations Of Ubiquitin Interactions with the Ubiquitin-Binding Domains of NEMO -- Generation of a Proteolytic Signal: E3/E2-mediated Polyubiquitination of IÎðBÎł -- Control of NF-ÎðB Subunits by Ubiquitination -- Methodology to Study NF-kB/RelA Ubiquitination in vivo -- Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-kB -- Methods to Detect NF-kB Acetylation and Methylation -- A Method for the Quantitative Analysis of Stimulation-Induced Nuclear Translocation of the p65 subunit of NF-kB from Patient-Derived Dermal Fibroblasts -- Methods for Assessing the In Vitro Transforming Activity of NF-ÎðB Transcription Factor c-Rel and Related Proteins -- Using RNA Interference in Lung Cancer Cells to Target the IKK-NF-ÎðB Pathway -- Immunohistochemical Analysis of NF-kB in Human Tumor Tissue -- Assessment of Canonical NF-kB Activity in Canine Diffuse Large B-Cell Lymphoma -- NEMO Binding Domain Peptide Inhibition of Inflammatory Signal-Induced NF-ÎðB Activation In Vivo -- Regulation of NFÎðB Signaling in Osteoclasts and Myeloid Progenitors -- NF-ÎðB Activation with Aging: Characterization and Therapeutic Inhibition -- The ́€œSneaking-ligand́€ Approach: Cell-type Specific Inhibition of the Classical NF-kB Pathway -- Sneaking-ligand Fusion Proteins Attenuate Serum Transfer Arthritis by Endothelium-targeted NF-kB inhibition -- Analysis of NF-kB Activation in Mouse Intestinal Epithelial Cells -- Characterizing the DNA Binding Site Specificity of NF-kB with Protein Binding Microarrays (PBMs) -- Methods for Analyzing the Evolutionary Relationship of NF-kB Proteins Using Free, Web-driven Bioinformatics and Phylogenetic Tools -- Studying NFkB Signaling with Mathematical Models
Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-ÎðB activation, regulation, and function.℗ NF-kappa℗ B: Methods and Protocols℗ guides readers through standard approaches to detect NF-ÎðB pathway activation, detection and analysis of℗ NF-ÎðB℗ signaling, and methods to study the control of℗ NF-ÎðB℗ signaling. As a volume in the highly successful Methods in Molecular Biology℗ series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative,℗ NF-Kappa℗ B: Methods and Protocols℗ provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-ÎðB signaling in health and disease
XVI, 668 p. 117 illus., 61 illus. in color. : online resource.