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  • 1
    Keywords: CELLS ; MICROSCOPY ; IMAGES ; PROTEINS ; PAIRS ; ANTIGEN ; ANTIGENS ; FIELD ; PARTICLES ; IDENTIFICATION ; US ; LOCALIZATION ; immunocytochemistry ; QUANTITATIVE-ANALYSIS ; FLUORESCENCE ; SECTIONS ; electron microscopy ; automated detection ; gold particles ; IMMUNOGOLD ; immunolabeling ; slow-scan cooled CCD camera
    Abstract: This work presents a computerized method to identify, detect, evaluate, and, by colored overlay, present gold particle pairs in electron microscopy (EM), even in wide-field views. Double gold immunolabeled specimens were analyzed in a LEO 912 electron microscope equipped with a 2k x 2k-pixel slow-scan cooled CCD camera connected to a computer with analySIS 3.1 PRO image processing software. The acquisition of a high-resolution and high-dynamic-range image by the camera allowed correct segmentation of the gold particles, separating them from other cell structures and from the substrate. Particle identification was performed by a classification module designed by us. Based on shape and size, the computer recognized the group of small particles and classified them as either singular or clustered and differentiated these from the single bigger type. The final image shows the particle types separated and colored, and indicates the total number of objects encountered in the specific region of interest. Moreover, a montage tool allowed us to obtain final representative images of large microscopic fields, which on analysis by the Gold Finder module provided information on the distribution and localization of antigens comparable to that provided by the wide-field light microscope images. (C) 2003 Elsevier Science (USA). All rights reserved
    Type of Publication: Journal article published
    PubMed ID: 12648569
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  • 2
    Keywords: brain ; DISTINCT ; GENES ; PROTEIN ; COMPLEX ; antibodies ; ARRANGEMENT ; ALPHA-TUBULIN ; ANCIENT EUKARYOTE ; BETA-TUBULIN ; cytoskeleton ; DIVERSITY ; Giardia lamblia ; immunocytochemistry ; MICROTUBULES ; POLYGLYCYLATION ; SUPERFAMILY ; TRYPANOSOMA-BRUCEI ; tubulin
    Abstract: Giardia lamblia is a diplomonad that parasitizes the small intestine of vertebrates. The trophozoite is multiflagellar and its cytoskeleton presents a complex organization of microtubular structures. One of these, the adhesive disk, consists of a microtubular spiral. The median body, whose function is not yet determined, is also composed by microtubules. The cell has eight flagella and two microtubule sheets, known as the funis. In this study we used several antibodies and immunofluorescence microscopy to help in the characterization of these structures. We observed that Giardia tubulin reacts with antibodies raised against very distinct immunogens. The antibodies used were against: (1) alpha-tubulin from chicken embryo brain, Trypanosoma brucei, sea urchin sperm, Paramecium, acetylated alpha-tubulin from Paramecium, and tyrosinated alpha-tubulin, (2) beta-tubulin from chicken embryo brain and Physarum polycephalum, and (3) an antibody with specificity to beta-tubulin having as immunogen the FtsZ bacterial protein. Each cytoskeletal structure of Giardia presented a distinct pattern of labeling by the several antibodies used. These data indicate that even being considered one of the most ancient of organisms, Giardia shares similarities (at least in relation to tubulin) with other organisms. They also open some questions about the organization and composition of its microtubular structures
    Type of Publication: Journal article published
    PubMed ID: 12687378
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