Springer Online Journal Archives 1860-2000
Summary While studying the distribution of acid mucopolysaccharides (AMPS) in the prepupal larva of the housefly, Musca domestica nebulo (Fahr.) using routine histochemical methods, the authors found that except for the midgut cells, dorsal pharyngeal epithelium and the thick connective tissue of the brain, the ganglia and the imaginal discs, in all other cells studied, an AMP is present which is neither a sulfomucin, a sialomucin nor a hyaluronic acid. Its alcian blue (AB) staining is abolished by active methylation and is not restored by saponification; it is not abolished by mild acid hydrolysis; it stains β-metachromatically with Azure A at pH 4.5 and lightly with Azure A at pH 1.5, with weak toluidine blue and with aluminium sulfate-methylene blue; it is unstained by aldehyde fuchsin (AF) and safranin O (Saf. O) in AB-AF, AF-AB and AB-Saf.O procedures; it is positive to colloidal iron but is negative to periodic acid Schiff reaction in all probability; and its AB staining is abolished by saponification (“KOH treatment”) without prior methylation. Pending further work to exactly characterize this AMP, it is tentatively referred to as “KOH-labile AMP”. The significance of its distribution is discussed.
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