Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Zeitschrift für Medizinische Ausbildung; VOL: 31; DOC19 /20140515/
    Publication Date: 2014-05-16
    Description: Aim: Student tutorials are now firmly anchored in medical education. However, to date there have only been isolated efforts to establish structured teacher training for peer tutors in medicine. To close this gap, a centralized tutor training program for students, culminating in an academic certificate, was implemented at Heidelberg University Medical School. The program also counts within the scope of the post-graduate Baden-Württemberg Certificate in Academic Teaching (Baden-Württemberg Zertifikat für Hochschuldidaktik).Method: Based on a needs assessment, a modular program comprised of four modules and a total of 200 curricular units was developed in cooperation with the Department for Key Competencies and Higher Education at Heidelberg University and implemented during the 2010 summer semester. This program covers not only topic-specific training sessions, but also independent teaching and an integrated evaluation of the learning process that is communicated to the graduates in the form of structured feedback. In addition, to evaluate the overall concept, semi-structured interviews (N=18) were conducted with the program graduates.Results: To date, 495 tutors have been trained in the basic module on teaching medicine, which is rated with a mean overall grade of 1.7 (SW: 0.6) and has served as Module I of the program since 2010. A total of 17% (N=83) of these tutors have gone on to enroll in the subsequent training modules of the program; 27 of them (m=12, f=15) have already successfully completed them. Based on qualitative analyses, it is evident that the training program certificate and its applicability toward the advanced teacher training for university instructors pose a major incentive for the graduates. For successful program realization, central coordination, extensive coordination within the medical school, and the evaluation of the attained skills have proven to be of particular importance.Conclusion: The training program contributes sustainably to both quality assurance and professionalism, as well as to solving the issue of resources in medical education. The introduction and continued development of similar programs is desirable.
    Description: Zielsetzung: Studentische Tutorien sind in der medizinischen Lehre inzwischen fest verankert. Bislang gibt es jedoch lediglich vereinzelte Bestrebungen, eine strukturierte medizindidaktische Ausbildung für studentische TutorInnen zu etablieren. Um diese Lücke zu schließen, wurde an der Medizinischen Fakultät Heidelberg ein zentrales didaktisches Qualifizierungsprogramm für TutorInnen implementiert, das mit einem universitären Zertifikat abschließt und im Rahmen des postgraduellen "Baden-Württemberg Zertifikat für Hochschuldidaktik" angerechnet wird. Methodik: Auf Basis einer Bedarfsanalyse wurde in Kooperation mit der Abteilung Schlüsselkompetenzen und Hochschuldidaktik der Universität Heidelberg ein modulares Programm bestehend aus vier Bausteinen und insgesamt 200 Unterrichtseinheiten (UE) entwickelt, das im Sommersemester 2010 implementiert wurde. Dieses beinhaltet sowohl themenspezifische Schulungen als auch eine eigenständige Lehrtätigkeit sowie eine integrierte Auswertung des Lernprozesses, welche den AbsolventInnen in Form eines strukturierten Feedback rückgemeldet wird. Zudem wurden halbstrukturierte Interviews (N=18) zur Bewertung des Gesamtkonzepts mit den AbsolventInnen geführt. Ergebnisse: Bislang wurden 495 TutorInnen im Grundlagenbaustein zu Medizindidaktik geschult, der mit einer durchschnittlichen Gesamtnote von 1,7 (SW: 0,6) bewertet wird und seit 2010 den Baustein I des Programms bildet. Insgesamt 17% (N=83) dieser TutorInnen haben sich seitdem in das aufbauende Qualifizierungsprogramm eingeschrieben, 27 von Ihnen (m=12, w=15) haben es bereits erfolgreich abgeschlossen. Anhand qualitativer Analysen wird ersichtlich, dass das übergeordnete didaktische Zertifikat und dessen Anrechenbarkeit im Rahmen der hochschuldidaktischen Ausbildung für Dozierende ein großer Anreiz für die AbsolventInnen ist. Für die erfolgreiche Implementierung erweisen sich insbesondere eine zentrale Koordinationsstelle, eine umfangreiche Abstimmung auf Fakultätsebene sowie die Auswertung der erworbenen Qualifizierung als wichtig.Schlussfolgerung: Das Qualifizierungsprogramm leistet einen nachhaltigen Beitrag zur Qualitätssicherung und Professionalisierung einerseits und zur Lösung der Ressourcenproblematik in der medizinischen Lehre andererseits. Die Einführung und Weiterentwicklung ähnlicher Programme ist wünschenswert.
    Keywords: Medical education ; peer teaching ; tutor training ; curriculum ; graduation certificate ; Medizindidaktik ; Peer Teaching ; TutorInnenqualifizierung ; Curriculum ; Abschlusszertifikat ; ddc: 610
    Language: German
    Type: article
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Jahrestagung der Gesellschaft für Medizinische Ausbildung (GMA); 20130926-20130928; Graz; DOCP13_10 /20130820/
    Publication Date: 2013-08-20
    Keywords: Didaktische Qualifizierung ; PAL ; Professionalisierung ; Übergeordnetes Zertifikat ; ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: PEPTIDE ; RECEPTOR ; CELLS ; EXPRESSION ; CELL ; Germany ; PROTEIN ; PROTEINS ; MONOCLONAL-ANTIBODY ; LIGAND ; MOTIFS ; FAMILY ; BINDING ; SEQUENCE ; SEQUENCES ; ALPHA ; ACID ; ACIDS ; antibodies ; antibody ; MUTANT ; VECTOR ; VIRUS-LIKE PARTICLES ; EXTRACELLULAR-MATRIX ; SURFACE ; BETA ; CRYSTAL-STRUCTURE ; PEPTIDES ; LIGANDS ; MONOCLONAL-ANTIBODIES ; INTEGRIN ; EPITOPE ; AMINO-ACIDS ; STRUCTURAL-CHANGES ; RECEPTORS ; CELL-SURFACE RECEPTOR ; MATRIX ; SUBSET ; MOTIF ; VIRIONS ; INSECT CELLS ; ADENOVIRUS RECEPTOR ; ALPHA(V)BETA(3) INTEGRIN ; COXSACKIEVIRUS A9 ; MOUTH-DISEASE VIRUS ; MURINE POLYOMAVIRUS ; POLYOMAVIRUS ; PROTEIN VP1
    Abstract: Integrins are a family of cell surface proteins that function as receptors for extracellular matrix ligands and for some viruses. A subset of integrins recognises peptide sequences containing arginine-glycine-aspartic acid (RGD) motifs as ligands. The B-lymphotropic polyomavirus (LPV) has a non-enveloped capsid that recognises a sialylated cell surface receptor. To change the receptor binding specificity we have replaced sets of three amino acids in three predicted surface loops of the major capsid protein VP1 of the B-lymphotropic polyomavirus LPV by RGD. Ten mutants gave rise to the expected 40 kDa VP1 protein upon expression from a baculovirus vector in insect cells. Five of the VP1 mutants representing all three surface loops have retained the ability to spontaneously assemble to capsids in the nuclei of the insect cells. Structural changes of the mutant capsid surface were shown by differential reactivity with a set of 7 neutralising monoclonal antibodies that recognise conformational surface epitopes of wildtype LPV virions. In addition all mutant capsids had lost specific binding to the LPV receptor. Three mutant capsids of one loop (BC) showed specific binding to alphavbeta3 integrin but not to integrins alphavbeta5, alphavbeta6, or to alphaIIbbeta3 known also to recognise RGD containing peptide sequences. This selective binding of the mutant capsids could be inhibited by synthetic peptides that specifically bind to alphavbeta3 integrin with IC50 values between 10 and 40 nM
    Type of Publication: Journal article published
    PubMed ID: 15290355
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Keywords: SIGNAL-TRANSDUCTION ; MAMMALIAN-CELLS ; LIPID RAFTS ; SINGLE-CELL ; reverse transfection ; PROTEIN PALMITOYLATION ; FAMILY KINASES ; CONTENT SCREENING MICROSCOPY ; AUTOMATIC IDENTIFICATION ; COATOMER COMPLEXES
    Abstract: SRC proteins are non-receptor tyrosine kinases that play key roles in regulating signal transduction by a diverse set of cell surface receptors. They contain N-terminal SH4 domains that are modified by fatty acylation and are functioning as membrane anchors. Acylated SH4 domains are both necessary and sufficient to mediate specific targeting of SRC kinases to the inner leaflet of plasma membranes. Intracellular transport of SRC kinases to the plasma membrane depends on microdomains into which SRC kinases partition upon palmitoylation. In the present study, we established a live-cell imaging screening system to identify gene products involved in plasma membrane targeting of SRC kinases. Based on siRNA arrays and a human model cell line expressing two kinds of SH4 reporter molecules, we conducted a genome-wide analysis of SH4-dependent protein targeting using an automated microscopy platform. We identified and validated 54 gene products whose down-regulation causes intracellular retention of SH4 reporter molecules. To detect and quantify this phenotype, we developed a software-based image analysis tool. Among the identified gene products, we found factors involved in lipid metabolism, intracellular transport, and cellular signaling processes. Furthermore, we identified proteins that are either associated with SRC kinases or are related to various known functions of SRC kinases such as other kinases and phosphatases potentially involved in SRC-mediated signal transduction. Finally, we identified gene products whose function is less defined or entirely unknown. Our findings provide a major resource for future studies unraveling the molecular mechanisms that underlie proper targeting of SRC kinases to the inner leaflet of plasma membranes.
    Type of Publication: Journal article published
    PubMed ID: 21795383
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Keywords: IN-VIVO ; GENE ; ALZHEIMERS-DISEASE ; IMMUNOREACTIVITY ; FRONTOTEMPORAL DEMENTIA ; CEREBROSPINAL-FLUID BIOMARKERS ; BUNINA BODIES ; CHROMOGRANIN PEPTIDES ; ALS PATIENTS ; EL-ESCORIAL
    Abstract: BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a fatal disorder of the motor neuron system with poor prognosis and marginal therapeutic options. Current clinical diagnostic criteria are based on electrophysiological examination and exclusion of other ALS-mimicking conditions. Neuroprotective treatments are, however, most promising in early disease stages. Identification of disease-specific CSF biomarkers and associated biochemical pathways is therefore most relevant to monitor disease progression, response to neuroprotective agents and to enable early inclusion of patients into clinical trials. METHODS AND FINDINGS: CSF from 35 patients with ALS diagnosed according to the revised El Escorial criteria and 23 age-matched controls was processed using paramagnetic bead chromatography for protein isolation and subsequently analyzed by MALDI-TOF mass spectrometry. CSF protein profiles were integrated into a Random Forest model constructed from 153 mass peaks. After reducing this peak set to the top 25%, a classifier was built which enabled prediction of ALS with high accuracy, sensitivity and specificity. Further analysis of the identified peptides resulted in a panel of five highly sensitive ALS biomarkers. Upregulation of secreted phosphoprotein 1 in ALS-CSF samples was confirmed by univariate analysis of ELISA and mass spectrometry data. Further quantitative validation of the five biomarkers was achieved in an 80-plex Multiple Reaction Monitoring mass spectrometry assay. CONCLUSIONS: ALS classification based on the CSF biomarker panel proposed in this study could become a valuable predictive tool for early clinical risk stratification. Of the numerous CSF proteins identified, many have putative roles in ALS-related metabolic processes, particularly in chromogranin-mediated secretion signaling pathways. While a stand-alone clinical application of this classifier will only be possible after further validation and a multicenter trial, it could be readily used to complement current ALS diagnostics and might also provide new insights into the pathomechanisms of this disease in the future.
    Type of Publication: Journal article published
    PubMed ID: 22970211
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  52. Kongress für Allgemeinmedizin und Familienmedizin; 20180913-20180915; Innsbruck, Österreich; DOC18degam203 /20180910/
    Publication Date: 2018-09-11
    Keywords: Feedback ; praktische Lehre Allgemeinmedizin ; studentische Lehre ; ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    Keywords: SURVIVAL ; CELL ; Germany ; MICROSCOPY ; screening ; GENE ; GENES ; GENOME ; RNA ; IDENTIFICATION ; ARRAYS ; HUMAN GENOME ; MIGRATION ; PHENOTYPE ; ORGANIZATION ; INTERFERENCE ; RNA INTERFERENCE ; RESOURCE ; SCIENCE ; LIFE ; TISSUE-CULTURE CELLS
    Abstract: Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the similar to 21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community
    Type of Publication: Journal article published
    PubMed ID: 20360735
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Keywords: GENOME ; FAMILY ; mechanisms ; INSTABILITY ; tubulin ; POSTTRANSLATIONAL MODIFICATIONS ; MITOTIC SPINDLE ; SUBUNIT NR3A ; PROTEIN 1S
    Abstract: Understanding the molecular basis for proper cell division requires a detailed functional analysis of microtubule (MT)-associated proteins. MT-associated protein 1S (MAP1S), the most ubiquitously expressed MAP1 family member, is required for accurate cell division. Here, using quantitative analysis of MT plus-end tracking, we show that MAP1S knockdown alters MT dynamics throughout the cell cycle. Surprisingly, MAP1S downregulation results in faster growing, yet short-lived, MTs in all cell cycle stages and in a global loss of MT acetylation. These aberrations correlate with severe defects in the final stages of cell division. In monopolar cytokinesis assays, we demonstrate that MAP1S guides MT-dependent initiation of cytokinesis. Our data underline the key role of MAP1S as a global regulator of MT stability and demonstrate a new primary function of MAP1S to regulate MT dynamics at the onset of cytokinesis.
    Type of Publication: Journal article published
    PubMed ID: 25300793
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Keywords: EXPRESSION ; GENE ; MICE ; IMMUNE-RESPONSES ; INTERFERON-GAMMA ; TUMOR-METASTASIS ; ANTIVIRAL DEFENSE ; NECTIN-2 CD112 ; DNAM-1 ; PVR CD155 ; REGULATORY FACTOR-I ; TRANSCRIPTION FACTOR IRF-1
    Abstract: IFN-gamma promotes tumoral immune surveillance, but its involvement in controlling metastases is less clear. Using a mouse model of pulmonary metastases, we show that local IFN-gamma treatment inhibits formation of metastases through its regulation of IRF-1 in tumor cells. IRF-1 is an IFN-gamma-induced transcription factor pivotal in the regulation of infection and inflammation. IRF-1 blockade abolished the inhibitory effect of IFN-gamma on tumor metastases, whereas ectopic expression of IRF-1 phenocopied the inhibitory effects of IFN-gamma. IRF-1 did not affect the survival of tumor cells in the circulation or their infiltration into lungs, but it was essential to support the pulmonary attraction and activation of natural killer (NK) cells. Depleting NK cells from mice abolished the protective effect of IFN-gamma or IRF-1 on metastases. In addition, cytotoxicity assays revealed that tumor cells expressing IRF-1 were targeted more effectively by NK cells than IRF-1 nonexpressing tumor cells. Moreover, NK cells isolated from lungs inoculated with IRF-1-expressing tumor cells exhibit a greater cytotoxic activity. Mechanistic investigations revealed that IRF-1-induced NK cell cytotoxicity was independent of perforin and granzyme B but dependent on the NK cell activating receptor DNAM-1. Taken together, our findings establish IRF-1 as an essential mediator of the cross-talk between tumor cells and NK cells that mediate immune surveillance in the metastatic niche.
    Type of Publication: Journal article published
    PubMed ID: 21900395
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Keywords: TRAFFICKING ; LOCALIZATION ; MAMMALIAN-CELLS ; REVEALS ; ENDOPLASMIC-RETICULUM ; MOLECULAR-MECHANISMS ; GLOBAL ANALYSIS ; TO-GOLGI TRANSPORT ; COPII VESICLE FORMATION ; RAB GTPASE
    Abstract: The secretory pathway in mammalian cells has evolved to facilitate the transfer of cargo molecules to internal and cell surface membranes. Use of automated microscopy-based genome-wide RNA interference screens in cultured human cells allowed us to identify 554 proteins influencing secretion. Cloning, fluorescent-tagging and subcellular localization analysis of 179 of these proteins revealed that more than two-thirds localize to either the cytoplasm or membranes of the secretory and endocytic pathways. The depletion of 143 of them resulted in perturbations in the organization of the COPII and/or COPI vesicular coat complexes of the early secretory pathway, or the morphology of the Golgi complex. Network analyses revealed a so far unappreciated link between early secretory pathway function, small GTP-binding protein regulation, actin cytoskeleton organization and EGF-receptor-mediated signalling. This work provides an important resource for an integrative understanding of global cellular organization and regulation of the secretory pathway in mammalian cells.
    Type of Publication: Journal article published
    PubMed ID: 22660414
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...