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  • 1
    ISSN: 1432-2242
    Keywords: Key words Disease resistance ; Hordeum vulgare ; Marker-assisted selection ; Molecular markers ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The genetic structure of the rym5 locus was studied in a population comprising 391 doubled-haploid lines that were evaluated for resistance to two strains of Barley Yellow Mosaic Virus (BaYMV-1, 2) and to Barley Mild Mosaic Virus (BaMMV). The absence of recombinants that are able to differentiate between the reaction to these different bymoviruses provides evidence that rym5 is a complex locus, which is either composed of several closely linked genes or of an allelic series of a single gene. For marker-assisted introgression of this locus into adapted barley germplasm, a CAPS (cleaved amplified polymorphic sequence) and a microsatellite marker were developed that flank the gene at distances of 0.8 and 1.3% recombination, respectively.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Hordeum vulgare s.l. ; Barley yellow mosaic disease ; Resistance ; RAPDs ; Genetic similarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Thirty-six Hordeum vulgare varieties and 12 H. spontaneum germplasms originating from different parts of the world and showing different reactions to the barley yellow mosaic virus complex (BaMMV, BaYMV, BaYMV-2) were analyzed for genetic similarity using RAPDs. On the basis of an analysis of 20 selected RAPD-primers corresponding to 544 bands genetic similarity according to Nei and Li (1979) was estimated to be between 0.685 and 0.964. Associations between the 48 genotypes were calculated using UPGMA-clustering and principal coordinate analysis. By applying these methods we were able to separate H. spontaneum accessions from H. vulgare varieties, and within these groups all the genotypes were clustered correctly according to their origin. Consequently, RAPD analysis can be considered a very useful and efficient tool for the fast estimation of genetic relationships in barley. The correlation between genetic similarity with respect to German varieties and adaptation of exotic barley varieties to German growing conditions is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Key words Barley Mild Mosaic Virus (BaMMV) ; Hordeum vulgare ; Molecular markers ; Marker-assisted selection ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In the present study three novel genes from barley accessions 10247 (ym8), Bulgarian 347 (ym9), and Russia 57 (ym11), which confer resistance to Barley Mild Mosaic Virus (BaMMV), were mapped using molecular markers. Bulked segregant analysis of four progenies segregating for resistance to BaMMV was followed by fine-scale mapping of the resistance genes using individual F2 or BC1F2 plants. The resistance genes are inherited recessively and are located on the long arm of barley chromosome 4HL. A series of closely linked molecular markers are available for marker-assisted breeding programs. A marker (MWG2134) linked with resistance gene ym11 from Russia 57 was identified, which is diagnostic for the resistance gene.
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  • 4
    ISSN: 1432-2242
    Keywords: Hordeum vulgare L. ; Barley mild mosaic virus (BaMMV) ; Resistance ; Mode of inheritance ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to obtain information about the mode of inheritance of BaMMV resistance in germplasms carrying genes different from the “German” gene ‘ym4’ f1-tests for resistance as well as F2-segregation analyses of crosses to susceptible German cultivars were carried out by mechanical inoculation in the greenhouse. In the f1 the majority of plants of each combination tested reacted susceptible to BaMMV while in the F2 a good fit to a segregation of 1r:3s or 7r:9s was observed. Therefore, the results of these tests revealed that the BaMMV resistance of all the varieties tested is inherited either by a single or by two recessive genes. By testing intercrosses of these resistant varieties segregation of BaMMV-susceptile plants was observed in the majority of combinations, revealing a high degree of genetic diversity in the barley gene pool.
    Type of Medium: Electronic Resource
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