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  • 1
    Call number: 0220:11 ; 04-Zell:312
    Keywords: Xenopus Laevis
    Pages: xvi,718p.
    ISBN: 0125641362
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    0220:11 departmental collection or stack – please contact the library
    04-Zell:312 departmental collection or stack – please contact the library
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  • 2
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To examine whether Ca2+ channels aggregate in a contact-dependent manner, we characterized the distribution of synaptic vesicles and postsynaptic receptors, and compared it to the location of Ca2+ entry sites, in a Xenopus laevis nerve-muscle coculture preparation using a localized Ca2+ detection method. The majority (75%) of Ca2+ entry sites at spontaneously formed nerve–muscle contacts were associated with enhanced immunofluorescence to the synaptic vesicle protein, SV2. In contrast, only 11% of recorded sites without Ca2+ transients exhibited significant SV2 immunofluorescence. When comparing the spatial distribution of synaptic markers with that of Ca2+ entry sites, we found that the majority of Ca2+ entry sites (61%) were associated with both enhanced SV2 immunofluorescence and R-BTX fluorescence, thereby identifying putative neurotransmitter release sites where Ca2+ channels, synaptic vesicles and postsynaptic receptors are colocalized. Using polystyrene beads coated with a heparin binding protein known to mediate in vitro postsynaptic receptor clustering, we show that the location of Ca2+ domains was associated with enhanced SV2 immunofluorescence at neurite-to-bead contacts. We conclude that the localization of functional Ca2+ channels to putative active zones follows a contact-dependent signalling mechanism similar to that known to mediate vesicle aggregation and AChR clustering.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 303 (1983), S. 61-64 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cells isolated from the dorsal part of Xenopus laevis embryos were cultured on coverslips10'11, and epithelial cells were identified according to published criteria . These cells were extremely thin and well spread with an average width of 125 jim (Fig. la). The periphery was often expanded into ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 292 (1981), S. 831-834 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1 Association of AChR clusters with beads Experiment Days of bead-muscle co-culture Beads AChR clusters (%)* Top/edge Bottom No. of clusters scored No. of cells scored + - + - A 2 Polylysine 96 3 0 1 283 22 Uncoated 12 64 0 24 175 22 Polycarboxylate 6 73 0 21 188 22 B ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 133 (1976), S. 57-71 
    ISSN: 1432-2048
    Keywords: Cell-wall formation ; Fucoid eggs ; Pelvetia ; Fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cell-wall formation in the egg of Pelvetia fastigiata (J.G. Agardh) DeToni (Fucaceae) was studied with freeze-fracture. 1. The wall is lamellated with microfibrils approximately parallel in each lamella. The average orientation of microfibrils turns about 35° in each subsequent lamella. This slow turn gives rise to bow-shaped arcs when the wall is obliquely cross fractured. 2. The organization of the fibrils in the innermost lamellae is visualized by their imprints on the plasma membrane. These imprints are the result of both turgor pressure and adhesion of fibrils to the membrane. 3. Strings of membrane particles appear on the plasma membrane shortly after fertilization. They seem to be formed by a fertilization-induced aggregation of isolated membrane particles. Later each string comes to lie under a fibril and along its imprint. Peculiar lateral rips indicate that some strings are tightly bound to a fibril and may be involved in its orientation. 4. Wall formation in Pelvetia is marked by pronounced secretory activities. Following fertilization, the fusion of cortical vesicles and other vesicles make numerous loci in the plasma membrane. In older embryos, fibril-free patches in the plasma membrane mark the position of microfibril elongation centers in the wall matrix. Prior to germination, these elongation centers and their corresponding membrane patches reach a high density at the presumptive rhizoid end.
    Type of Medium: Electronic Resource
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