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  • 1
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  PTCOG 48; Meeting of the Particle Therapy Co-Operative Group; 20090928-20091003; Heidelberg; DOC09ptcog107 /20090924/
    Publication Date: 2009-09-25
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 2
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    German Medical Science GMS Publishing House; Düsseldorf
    In:  64. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC); 20130526-20130529; Düsseldorf; DOCDI.09.07 /20130521/
    Publication Date: 2013-05-22
    Keywords: glioma ; PET ; survival ; ddc: 610
    Language: English
    Type: conferenceObject
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  • 3
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    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  64. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC); 20130526-20130529; Düsseldorf; DOCDI.05.09 /20130521/
    Publication Date: 2013-05-22
    Keywords: FET-PET ; anaplastic glioma ; MRI imaging ; ddc: 610
    Language: English
    Type: conferenceObject
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  • 4
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Procedures for ultrastructural postembedment immunolocalization of antigens were investigated by use of the indirect peroxidase labelled antibody method. Results were compared with those obtained with the ultrastructural preembedment technique. IgG globulins in IgG synthesizing cells of rat lymph nodes served as model. Formaldehyde fixation, ethanol dehydration and embedment in Epon 812 did not abolish immunoreactivity. Sufficient numbers of antigens remained for subsequent postembedment immunohistology. Antigen binding sites were readily localized in ultrathin sections. For this purpose, polymerized resin had to be partially removed. Sodium methoxide in methanol/benzene mixture rapidly dissolved ultrathin sections. Diluted alcoholic sodium hydroxide enabled reliable resin etching and subsequent immunostaining. Treatment of ultrathin sections with hydrogen peroxide alone, did not permit immunolocalization of IgG. Optimal antigen detection was attained with antibodies isolated by affinity chromatography and purified peroxidase conjugates. IgG was stained in the rough-surfaced endoplasmic reticulum, the perinuclear space and the Golgi apparatus; the subcellular distribution corresponded to that obtained with preembedment immunohistology. In the latter technique, substrate accumulation was more homogenous than in postembedment staining.
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  • 5
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The histological localisation of α-D-galactopyranosyl residues in glycoconjugates of rat stomach and duodenal mucosae was studied by use of Griffonia simplicifolia agglutinin I, i.e. the isolectin mixture (A+B) and the isolectin B4 (B4). Cryostat sections which were either unfixed or acetone fixed and paraffin sections from both ethanolacetic acid and formaldehyde fixed tissue blocks were compared. Cellular details were better preserved in paraffin than in cryostat sections. Reactivity of cells binding GS I was less sensitive after formaldehyde than after ethanol-acetic acid fixation inasmuch as higher concentrations of lectins were needed. This drawback could be overcome by trypsinisation of the sections. The binding pattern of GS I (A+B) corresponded with that of GS I (B4) in either cryostat or paraffin sections. GS I was detected in the cytoplasm of parietal cells and in Brunner's gland cells. In duodenal crypts and villi, lectin was bound to supranuclear regions in the cytoplasm of columnar and goblet cells. The staining efficiency of fluorescein (FITC), horseradish peroxidase (HRP) and colloidal gold particle (CGP) labels in both direct and indirect lectin stainings was compared. Under all experimental conditions, indirect methods required lower concentrations of lectins than direct ones; indirect procedures increased sensitivity about 5–10 fold. CGP labels were always of highest sensitivity when gold particles were further developed by a silver precipitation method. HRP was not as efficient in lectin localisation as CGP, but cytochemical staining was more convenient in routine work. Direct FITC labellings proved to be of lowest sensitivity.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 82 (1985), S. 411-419 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Universal, polyclonal and monoclonal immunoperoxidase staining kits from BioGenex, Dako and Ortho were employed for the localization of antigens such as gastrin, prostate specific antigen, IgA, IgG, AFP and CEA in histological sections from formaldehyde fixed and paraffin embedded human specimens. The kit components were controlled by immunohistological and serological assays and were also compared with self-prepared reagents. In connection with specific primary antibodies, universal/basic kits gave reliable localization of defined antigens. The optimal concentration of the primary antibodies had to be established by dilution experiments. In the case of polyclonal kits, typical antigen localization was obtained in selected tissue sections with all the respective kits. CEA kits also stained strongly NCA molecules present in organs such as colon, stomach and liver. BioGenex polyclonal kits gave almost stronger stainings than kits from Dako and Ortho. Irrespective of which kit from different commercial sources is used, development of peroxidase activity with AEC/H2O2 often had to be stopped far below the recommended incubation time of 40 min or overstaining with color change from reddish to muddy green occurred. The latter was attributed to insufficiently balanced kit reagents, an interpretation wich was supported by quantitative serological studies. Sensitivity of immunohistological reactivity was much enhanced by pretreatment of tissue sections with Pronase. Thus, stronger immunostainings and larger numbers of positive cells were detected than in conventionally rehydrated sections. Incubation of sections with self-prepared primary antibodies, linking antibodies and PAP complexes gave essentially the same antigen localization as with commercial kits, but antibodies isolated by our affinity chromatography led to a better staining contrast with absence of nonspecific background. The advantage of monoclonal over polyclonal kits was the background-free staining of sections. Other-wise, antigens were localized in the same cell types, although cellular reactivity was usually less intense than with polyclonal antibodies. This, however, could be overcome by Pronase treatment of the sections prior to incubation.
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  • 7
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A sensitive staining procedure for glucose oxidase (GOD) as marker in immunohistology is described. The cytochemical procedure involves a two-step enzyme method in which GOD and horseradish peroxidase (HRP) are coimmobilized onto the same cellular sites by immunological bridging or by the principle of avidin-biotin interaction. In this coupled enzyme technique, H2O2 generated during GOD reaction is the substrate for HRP and is utilized for the oxidation of chromogens such as 3,3′-diaminobenzidine or 3-amino-9-ethylcarbazole. Due to the immobilization of the capture enzyme HRP in close proximity to the marker enzyme (GOD), more intense and specific staining is produced than can be obtained with soluble HRP as coupling enzyme in the substrate medium. Indirect antibody labelled and antibody bridge techniques including the avidin (streptavidin)-biotin principle have proven the usefulness of this GOD labelling procedure for antigen localization in paraffin sections. Antigens such as IgA in tonsil, alpha-feroprotein in liver and tissue polypeptide antigen in mainmary gland served as models. The immobilized twostep enzyme procedures have the same order of sensitivity and specificity as comparable immunoperoxidase methods. The coupled GOD-HRP principle can be superior to conventional immunoperoxidase labelling for the localization of biomolecules in tissue preparations rich in endogenous peroxidase activities.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 398 (1983), S. 319-328 
    ISSN: 1432-2307
    Keywords: Lectins ; Glycoconjugate ; Gastric mucosa ; Histopathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Peroxidase (HRP) conjugates ofDolichos biflorus agglutinin (DBA),Arachis hypogaea agglutinin (PNA) andUlex europaeus agglutinin I (UEA I) were used as specific molecular probes in order to describe histological patterns of carbohydrate moieties in glycoconjugates of gastric mucosa. Specimens of normal mucosa, metaplasias and carcinomas were fixed, embedded in paraffin and stained with lectins by a postembedding technique. In normal mucosa, DBA reacted with surface epithelial, parietal and antral gland cells; neck cells did not react. In single goblet cell metaplasia, with few exceptions, goblet cells showed DBA binding. In contrast, mainly negative goblet cells were seen in intestinal metaplasia. In gastric carcinomas of intestinal or diffuse types, heterogenous staining patterns were observed. Large numbers of mucus-producing surface epithelial, neck and antral gland cells stained with PNA-HRP conjugates. Parietal cells did not react. Among the chief cells only few showed PNA binding. In single goblet cell metaplasia, equal numbers of goblet cells were positive or negative, whereas goblet cells in intestinal metaplasia were mainly unstained. Carcinomas contained both PNA-positive and PNA-negative cells; PNA-positive cells occurred preferentially in the tumor periphery. UEA I — HRP conjugates stained the bulk of surface epithelial and neck cells; chief cells and antral gland cells were mainly positive, too. Parietal cells did not react. In single goblet cell metaplasia and intestinal metaplasia, UEA I-positive as well as UEA-negative goblet cells occurred. Striated cells exhibited a great variety of lectin reactivity. Carcinomas also showed a heterogenous staining pattern. Under both normal conditions and in carcinoma development, qualitative and quantitative differences in the composition of glycoconjugates must be expected.
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  • 9
    ISSN: 1432-2307
    Keywords: Lectins ; Glycoconjugates ; Duodenal mucosa ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Peroxidase (HRP) conjugates ofArachis hypogaea agglutinin (PNA),Dolichos biflorus agglutinin (DBA),Ulex europaeus agglutinin I (UEA I) andBandeiraea simplicifolia agglutinin I (BSA I) were used to detect the expression of carbohydrate moieties in glyco-conjugates of normal human duodenal mucosa. Lectin histology was performed on sections from paraffin embedded specimens of donor blood groups A, B, AB and O. While PNA, DBA and UEA I staining appeared to be independent of the donor blood groups, BSA I affinity was restricted to the blood groups B and AB. Neither striated columnar cells nor goblet cells stained with PNA-HRP conjugates. Glyco-conjugates of goblet cell mucins reacted with DBA and UEA I, but staining intensities varied in crypts and villi: positive goblet cells occurred preferentially in upper parts of the crypts and in villi. Striated columnar cells also exhibited variation in staining intensities, with binding of DBA and UEA I observed in the upper half of crypts and in villi, occurring predominantly along the brush border and in apical parts of the cells. Glyco-conjugates synthesized by Brunner's gland cells possessed a great variety of lectin reactivity with the lectins employed. Within a given histological section positive gland areas with different staining intensities beside negative cell populations might reflect different functional states.
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  • 10
    ISSN: 1432-2307
    Keywords: Goblet cell antigen ; Immunoenzymehistology ; Intestinal metaplasia ; Gastrointestinal cancer ; Common stem cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Goblet cell antigen (GOA1) was purified from gastric signet ring cell carcinoma. It was immunogenic and was used to produce antisera which stained goblet cells of the small and large intestine and of intestinalized gastric mucosa by indirect immunological methods. Various types of gastric and colonic cancer contained GOA1. These findings demonstrate a histiogenic relationship between intestinal goblet cells, various gastrointestinal cancers and associated premalignant conditions.
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