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  • 1
    Subject(s): Biophysics. ; Biotechnology. ; Biophysics. ; Biotechnology.
    In: Springer Nature eBook
    Description / Table of Contents: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via .
    Type of Medium: Online Resource
    Pages: XIV, 511 p. 130 illus., 109 illus. in color. , online resource.
    Edition: 3rd ed. 2024.
    ISBN: 9781071633779
    Series Statement: Methods in Molecular Biology, 2694
    Language: English
    Note: Introduction to Optical Tweezers: Background, System Designs, and Applications -- Quantifying ATP-Independent Nucleosome Chaperone Activity with Single Molecule Methods -- Protein Tethering for Single-Molecule Force Spectroscopy -- Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies -- Probing Mitotic Chromosome Mechanics Using Optical Tweezers -- A Brief Introduction to Single-Molecule Fluorescence Methods -- Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans -- Lattice Light-Sheet Motor-PAINT: A Method to Map the Orientations of Microtubules in Complex Three-Dimensional Arrays -- Fluorescence Microscopy of Nanochannel-Confined DNA -- Single-Molecule FRET X -- Single-Molecule Fluorescence Imaging of DNA Replication Stalling at Sites of Nucleoprotein Complexes -- Measuring Transcription Dynamics of Individual Genes Inside Living Cells -- Single-Molecule FRET Resolved Protein Dynamics from Plasmid to Data in Six Steps -- Atomic Force Microscopy: An Introduction -- Atomic Force Microscopy of Viruses: Stability, Disassembly, and Genome Release -- Unfolding and Refolding Proteins using Single-Molecule AFM -- Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy -- An Introduction to Magnetic Tweezers -- Surface Functionalization, Nucleic Acid Tether Characterization, and Force Calibration for a Magnetic Tweezers Assay -- Correlated Single-Molecule Magnetic Tweezers and Fluorescence Measurements of DNA-Enzyme Interactions -- Detecting DNA Loops Using Tethered Particle Motion -- Single-Cell Measurements using Acoustic Force Spectroscopy (AFS) -- DNA Origami-Based Single-Molecule Force Spectroscopy and Applications.
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  • 2
    Publication Date: 2020-08-01
    Print ISSN: 0006-291X
    Electronic ISSN: 1090-2104
    Topics: Biology , Chemistry and Pharmacology , Physics
    Published by Elsevier
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  • 3
    Publication Date: 2020-03-01
    Print ISSN: 0960-9822
    Electronic ISSN: 1879-0445
    Topics: Biology
    Published by Cell Press
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  • 4
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Physical Chemistry 55 (2004), S. 79-96 
    ISSN: 0066-426X
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Chemistry and Pharmacology , Physics
    Notes: The methods of single-molecule fluorescence spectroscopy and microscopy have been recently utilized to explore the mechanism of action of several members of the kinesin and myosin biomolecular motor protein families. Whereas ensemble averaging is removed in single-molecule studies, heterogeneity in the behavior of individual motors can be directly observed, without synchronization. Observation of translocation by individual copies of motor proteins allows analysis of step size, rate, pausing, and other statistical properties of the process. Polarization microscopy as a function of nucleotide state has been particularly useful in revealing new and highly rotationally mobile forms of particular motors. These experiments complement X-ray and biochemical studies and provide a detailed view into the local dynamical behavior of motor proteins.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Kinesin is an ATP-driven molecular motor protein that moves processively along microtubules. Despite considerable research, the detailed mechanism of kinesin motion remains elusive. We applied an enhanced suite of single- and multiple-molecule fluorescence polarization microscopy assays to report ...
    Type of Medium: Electronic Resource
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