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  • 1
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A serological survey using alloantisera specific for the H-2 class I antigens in Japanese wild mice,Mus musculus molossinus, revealed a high frequency of the H-2Kf antigen. This antigen has also been found in European wild mice,M. m. domesticus andM. m. musculus. In this survey, the H-2Kf antigen was characterized through the use of ten newly isolated monoclonal antibodies raised against cells of a Japanese wild mouse, and by Southern blot analysis using anH-2K locus-specific probe which hybridizes with the 3′ end of the gene. The serologically identified H-2Kf antigens revealed several minor variations in reactivities to the monoclonal antibodies. However, all the antigens examined could be clearly separated into two types with respect to the restriction fragment length polymorphism (RFLP) pattern. The first type, found together with a single, characteristic RFLP pattern, was always associated with the presence of reactivity to one particular monoclonal antibody, MS54. The second type, found to represent different RFLP patterns, is associated with the absence of reactivity to MS54. This concordance between the presence of an antigenic determinant and a particular RFLP was observed not only withinMus musculus subspecies but also in a different species:M. spretus, carrying the same antigenic determinant, gave an identical RFLP to that of the other MS54-positiveMus musculus subspecies. The data suggest that the antigenic determinant specific for MS54 is an ancient polymorphic structure which has survived the long period of diversification ofMus species (approximately 2–3 million years) without alteration, and is associated with a stable DNA structure at the 3′ end of theH-2K gene.
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  • 2
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have already developed nine B10.MOL congenic strains carrying H-2 haplotypes derived from Japanese wild mice, Mus musculus molossinus, with the C57BL/10 genetic background. To obtain monoclonal antibodies against the H-2 antigen of the Japanese wild mouse, we carried out cell fusion using spleen cells from the animal immunized with one of the B10.MOL strains, B10.MOL-SGR (H-2 wm7). As a result, 19 hybridomas producing monoclonal antibodies were produced. Analysis with the intro-H-2 recombinants derived from B10.MOL-SGR indicated that 8 of them reacted with the class I and II with the class II molecule. The class I antibodies were tested for their cross -reactivities on wild mice and on the panels of standard inbred and B10.MOL strains. Most of the antibodies reacted with both the Japanese wild mice and the other subspecies, including standard inbred, while two antibodies highly specific for the donor H-2K region reacted with only three wild-derived mice, two M. m. molossinus from Anj o and Shizuoka, Japan, and one M. m. domesticus from Pigeon, Canada. In addition, all of the other four antibodies reactive with the K antigen of B10.MOL-SGR also reacted with the same three wild mice. The wild mice belonging to different subspecies might share very similar H-2K antigenic determinants in spite of their genetic and geographical remoteness.
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  • 3
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Allelic distributions of Thy-1, Ly-l, and Ly-2 antigens in wild mice are characteristic of each Mus musculus subspecies. Eastern mice (M.m.molossinus, M.mmusculus, M.m.castaneus, M.m.bactrianus) express the Thy-1.1 antigen, whereas Western mice (M.m. domesticus, M.m.brevirostris) express the Thy-1.2. All mice from wild populations examined in this survey express the Ly-1.2. The Ly-2.1 is distributed in Eastern mice and some Western mice, and the Ly-2.2 is found in the remaining Western mice. Allelic distributions of these antigens were also examined in two other species, Mus spretus and Mus spicilegus. Allelic constitutions of Thy-1 and Ly-1 in these species are similar to those of Eastern mice. Some M.spicilegus, however, express the Ly-1.1 antigen. This antigenic type is not found in M.musculus. Some Eastern mice related to M.m.castaneus react weakly to Ly-1.2-specific and Ly-2.1-specific monoclonal antibodies in both the complement-mediated cytotoxicity test and the absorption test. These results suggest that M.m.castaneus has unique alleles in the Ly-1 and Ly-2 loci.
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  • 4
    ISSN: 1573-4927
    Keywords: one-dimensional electrophoresis ; two-dimensional electrophoresis ; polyacrylamide gel electrophoresis ; genetic variability ; erythrocytic proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Comparisons of the sensitivities of one-dimensional (1D) and two-dimensional (2D) electrophoreses to detect genetic variability have generally shown that the 2D approach appears to be two- to five-fold less sensitive than conventional 1D approaches. Concerns about the validity of this conclusion have arisen because such comparisons have involved mainly enzymic proteins in 1D approaches versus a complex mixture of soluble proteins in most 2D analyses. Comparisons involving the absolute number of variants detected, using 1D and 2D sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), denatured mouse blood proteins isolated from C3HeB/FeJ and C57 B1/6J inbred strains of mice, and highly sensitive silver staining, indicate that the latter uncovers at least as much variability as the former. Although the relative percentage of variable bands (1D SDS-PAGE) was greater than the relative percentage of variable spots (2D SDS-PAGE) when proteins of intact erythrocytes were surveyed, both techniques uncovered approximately equal percentages of variable proteins when the mouse erythrocyte proteins were partitioned into membrane and lysate components. Therefore, the simpler 1D SDS-PAGE was found to be as effective as 2D SDS-PAGE in detecting protein variability. Since 1D SDS-PAGE separates proteins primarily on the basis of molecular weight and to a lesser degree on other primary protein sequence alterations, much of the variability observed by 2D SDS-PAGE may be due to these same features and unit charge differences may not play a significant role in detecting variability in the proteins studied. This differs from enzymic proteins, where such charge differences appear to be responsible for much of the variability. This study also indicated that decreasing the number of proteins in samples (membranes and lysates vs whole erythrocytes) increased the ability of both of these techniques to resolve differences. Mating studies indicated that most of the differences detected with both techniques were inherited and were not artifacts.
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  • 5
    ISSN: 1573-4927
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electrophoretic separation of house mouse (Mus musculus) hemoglobins on starch gel in a 0.2 M tris maleate NaOH pH 7.0 buffer facilitates the recognition of the three phenotypes controlled by locus Hbb. Evidence that Hbb does control the electrophoretic patterns with this buffer system is discussed.
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  • 6
    ISSN: 1573-4927
    Keywords: serum albumin ; mouse ; electrophoretic variant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An electrophoretic variant for serum albumin in Mus musculus has been used to map the structural gene for this protein to chromosome 5.
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