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  • 1
    ISSN: 1432-2307
    Keywords: Eosinophilic granuloma ; Frontal lobe ; Histiocytosis-X ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The clinical symptoms, pathological findings and surgical treatment of a case of primary frontal lobe eosinophilic granuloma are presented. Initially a frontal mass was detected that clinically seemed like a low-growth tumor. After operation, the histological, cytochemical and ultrastructural studies showed positive acid phosphatase, α-naftil-esterase, oil-red and PAS reactions of tumoral cells and the presence of rod-shaped bodies in proliferative histiocytes, all which confirmed the pathological diagnosis of eosinophilic granuloma. Other osseous or visceral histiocytosis-X signs were not observed. The patient remains asymptomatic after a postoperative follow-up of 2 years.
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  • 2
    ISSN: 1432-2307
    Keywords: Human germ cell tumour of testis ; Cell culture ; Neural differentiation ; Myoblastic differentiation ; Cytogenetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Non-seminomatous germ cell tumours of the testis (NSGCT) form a heterogeneous group of neoplasms. Cell lines derived from NSGCT may provide useful data concerning the biology of neoplasic precursor germ cells, differentiation of tumour stem cells and the relationship between various tissue components of these tumours. Four NSGCT were studied, two mixed tumours composed of teratocarcinoma, yolk sac and trophoblastic elements, and two malignant teratomas with a massive neuroectodermal component, equivalent to primary neuroectodermal tumours (PNET) of the testis. The explanted tumours gave rise to various cell populations, including epitheloid cells, flattened large cells, spindle cells and tear drop cells of neuroblastic type. Ultrastructurally, cultured cells expressed various degrees of neural and muscular differentiation: neurosecretory granules, intermediate filaments of glial nature, and filaments resembling Z-bands. Cultured cells showed the expression of several neural and muscular markers, including neurofilaments, cytokeratin, actin, desmin, neuron-specific enolase, glial fibrillary acidic protein and HNK-1. In addition, three cases expressed HBA-71 antigen and two expressed MyoDI protein. All cases were aneuploid, and an isochromosome 12p, i(12p), was detected in three cases. Myoblastic and neural cells are the predominant tumour cells that grow in vitro, independent of the nature and composition of the primary germ cell tumour. A histogenetic relationship between germ cell tumours and small round cell tumours of childhood is suggested.
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  • 3
    ISSN: 1432-2307
    Keywords: Neuroectodermal bone tumours ; Immunochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Peripheral neuroectodermal tumours (PNET) of bone are rare and mimick those seen in soft tissue (peripheral neuroepithelioma of soft tissue). Their differential diagnosis from Ewing's sarcoma (Es) is extremely difficult by optical means. Here we report 14 new cases of PNET of bone (other than Askin's neoplasm) located primarily in the limbs, pelvic girdle and scapula. Clinically and radiologically they displayed Ewing's sarcoma-like features: mean age was 14.4 years, male/ female ratio being 3:11. Metastasis was present in 6 cases at diagnosis (5 with bone metastasis). Prognosis was poor; thirteen patients died; only one with a metatarsal located tumour is alive and free of disease. The mean survival rate was 25 months following diagnosis and treatment with radio- and multimodal chemotherapy. Histologically the 14 cases displayed Homer-Wright rosettes and pseudorosette-like structures, as well as a fibrillary background and lobular pattern. Immunohistochemistry revealed positivity in a number of neural markers when using paraffin-embedded material: NSE, B-2-microglobuline, HNK-1 (leu-7) and E-36 antibodies. At EM level the cell cytoplasms evidenced dense-core granules with neurosecretion, neurotubules and intermediate filaments like those seen in peripheral neuroepithelioma.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 362 (1974), S. 13-21 
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The microscopic morphology, histochemistry and electron microscopy of a non-ossifying fibroma of the upper metaphysis of the femur in a sixteen-year-old girl, is presented. The authors describe a fibroblastic cell type as the basis of the neoplasia which transforms itself into a foam cell loaded with lipids. Histochemically it is characterized by high activity in alkaline phosphatases, ATP-ases, fructose 1–6 diphosphatase and NADH-NADPH tetrazolio reductases. Electron microscopy identifies extremely active fibroblastic cells with a synthesis of proteic and lipid material which transforms them into foam cells. The lipids appear irregularly enveloped in laminar systems. There is also a deposit of hemosiderine in certain fibroblasts. Non-ossifying fibroma differs from the metaphyseal fibrous bone defect as the fibroblasts show a higher activity for alkaline phosphatase and lipids are stored in the cytoplasma.
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  • 5
    ISSN: 1432-2307
    Keywords: Hemangioendothelioma ; Esophagus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The histology and electron-microscopy of a malignant hemangioendothelioma of the esophagus wall appearing in a 42 year old male is presented. By light microscopy the tumor is composed of vessels and capillary-like structures of an anastomosing nature covered by atypical endothelial cells. These cells infiltrate the intersticial spaces growing into the posterior mediastinal area. Electron microscopy confirms the endothelial nature of the neoplastic cells, showing characteristics of the cell type, as is the presence of Weibel-Palade bodies, filaments and active pinocytosis. Hemangioendothelioma should be differentiated from other vascular tumors (angiosarcoma) as are hemangiopericytoma or hemangioblastoma, being composed exclusively of malignantly transformed endothelial cells.
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  • 6
    ISSN: 1432-2307
    Keywords: Ewing's sarcoma ; Transmission electron microscopy (TEM) ; Scanning electron microscopy (SEM)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Scanning electron microscopy (SEM) is of value for the differential diagnosis of Ewing's tumor of bone. Based upon 9 new cases which were observed with SEM and TEM (transmission electron microscopy), this paper puts into consideration, for the first time, the SEM ultrastructure of Ewing's sarcoma (both variants; typical Ewing's sarcoma and the large cell Ewing's sarcoma). Furthermore, a new case of vascular Ewing's sarcoma, studied with TEM, is discussed and included in the differential diagnosis with other round cell sarcomas of bone. Both Ewing's sarcoma types evidence common ultrastructural characteristics, but the atypical variant (large cell type) shows a greater variation in cell size and contour. The cell surfaces displayed smooth structures, interrupted only by clusters of short, stub-like microvilli. Isolated cilia were also observed. Variations in cell contour and size within the same tumour are also induced through intensive chemotherapy, as noted in one of our cases. SEM seems to be suitable for the differentiation of Ewing's tumours from other primary malignancies of the bone marrow, as is the case of the so-called “reticulum cell sarcoma of bone” or malignant non-Hodgkin lymphoma. SEM studies associated with TEM give further support to the mesenchymal origin of this neoplasm.
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  • 7
    ISSN: 1432-2307
    Keywords: Esthesioneuroblastoma ; Neurosecretion ; Melanosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Esthesioneuroblastoma (ESTH) is a neuroepithelial-cell-derived neoplasm of the olfactory mucosa composed of homogeneous small round cells which contain neurosecretory granules. Melanin has been detected in such tumours only occasionally. Here we describe a new case of ESTH with divergent differentiation. The primary neoplasm was found in a 67 year-old female, involving the left nasal and maxillary sinus; she died of cerebral metastasis ten months after diagnosis. Histologically only small round cells were seen, with S-100 and NSE positivity. Electron microscopy revealed neurosecretory granules and filaments, as well as the occasional presence of melanosomes. A nude mice xenograft line has been established, and is presently in its ninth transfer. Two cell types are present: small round-to-spindle shaped cells with neural features, and large epithelial-like ones. Both immunohistochemistry and electron microscopy confirm this dual differentiation, with the presence of membrane-bound dense-core neural secretion, as well as melanosomes of neuroectodermal origin. Additionally, an in vitro cell line has been established. Cytogenetic analysis confirmed the presence of both malignant human melanoma patterns; non-random abnormalities in chromosomes 1 and 6, extra copies of chromosome 7. Duplication of the long arm of chromosome 14, as seen in olfactory neuroblastoma, is also seen.
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  • 8
    ISSN: 0165-4608
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0165-4608
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Twenty-seven cases of Wilm's tumour were reviewed in order to confirm the presence of nervous tissue which could imply a ‘neuroepithelial’ origin for these neoplasms. For this purpose a double-silver impregnation technique was used. Groups of ganglion cells with neurofibrils and non-myelinated axon-like processes appeared associated with a fine neurofibrillar network with neuromuscular junctions. Nervous tissue in nephroblastoma is mature and should not be confused with primary ganglioneuroblastoma of the kidney in which neuroblasts in rosette-like groupings are very numerous. A case report of such a lesion is discussed for purposes of comparison.
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