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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Riassunto E'stata proposta, per il nucleo ed i cromosomi, una reazione con argentoesa-metilentetrammina, tamponata con borace, previa idrolisi acida con HC1 1N, a caldo, e ne è stata saggiata la specificità mediante digestioni enzimatiche (DNAasi, pepsina, tripsina) e confronto dei risultati ottenuti con la reazione di Peulgen. Questa colorazione presenta il vantaggio, rispetto alle usuali colorazioni, di un notevole contrasto fotografico. Inoltre la non colorabilità delle strutture nucleari, dopo digestione con DNAasi, lascia supporre una specificità del metodo per le strutture contenenti DNA.
    Notes: Summary A staining reaction for the nucleus and chromosomes, using silver-hexamethylentetramine, buffered with borax, after hydrolisis with warm IN HC1, has been proposed. Its specificity has been assayed by means of enzymatic digestions (DNase, pepsin, trypsin), and through the comparison of the results with those obtained by the Feulgen reaction. This staining method bears the advantage, compared to the usual methods, of a noteworthy photographic contrast. Moreover this method is probably specific for the DNA containing structures, because of the lack of stainability of nuclear structures, after DNase digestion.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Kidney ; Contractile structures ; Embryonic metanephros ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytoplasmic filaments have been observed in the cells of normal and pathological kidneys. These filaments are usually grouped into bundles anchored to electron dense bodies underlying the cell membrane. In the embryonic human metanephros the filaments are found within the cells of different portions of the nephron at various stages of development. They appear first in the podocytes, almost simultaneously in the Bowman's capsule and tubular cells, then in the “mesangial cells”, and finally in the cells of the media of the afferent glomerular and interlobular arterioles. The presence of filaments and their attachment bodies in the mammalian nephron suggests that the podocytes and the so-called mesangial cells have a contractile activity, thus representing an intraglomerular apparatus which regulates the intravascular pressure, blood flow and filtration rate in the glomerular capillaries, whilst the contractile activity of the Bowman's capsule and proximal, distal, and collecting tubules, could facilitate the progression of the filtrate. The increase in number of the filaments in some pathological conditions is probably related to the functional changes of the intraluminal pressure in the glomerular capillaries, in the Bowman's space, and in the tubular lumen.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0022-5320
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Ultrasructure Research 22 (1968), S. 195-205 
    ISSN: 0022-5320
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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