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  • 1
    Publication Date: 2018-02-20
    Description: Aims Diagnostic ambiguities regarding the malignant potentiality of oral submucous fibrosis (OSF), an oral precancerous condition having dysplastic and non-dysplastic isoforms are the major failure for early intervention of oral squamous cell carcinoma (OSCC) patients. Our goal is to identify proteomic signatures from biopsies that can be used as precancer diagnostic marker for patient suffering from OSF. Methods The high throughput techniques adopting de novo peptide sequencing (1D SDS-PAGE coupled nanoLC MALDI tandem mass spectrometry (MS/MS)-based peptide mass fingerprint), immunohistochemistry (IHC), Western blot (WB) and real-time PCR (RT-PCR) analysis are considered for such biomarker identification and multilevel validations. Results Alpha-enolase is identified as an overexpressed protein in biopsies of oral submucous fibrosis with dysplasia (OSFWD) compared with oral submucous fibrosis without dysplasia (OSFWT) and normal oral mucosa (NOM). Total proteome analysis of an overexpressed protein band around 47 kDa of OSFWD identifies 334 peptides corresponding to 61 human proteins. Among them α-enolase is identified as a prime protein with highest number of peptides (44 out of 334 peptides) and sequence coverage (66.4%). Furthermore, RT-PCR, WB and IHC analysis also show mRNA and tissue level upregulation of α-enolase in OSFWD validating α-enolase as precancer marker. Conclusions This study for the first time identifies and validates α-enolase as a novel biomarker for early diagnosis of malignant potentiality of OSF. Hence, the identified protein marker, α-enolase can help in early therapeutic intervention of OSF patients leading to the reduction of patient’s pain, treatment cost and enhancement of patient’s quality of life.
    Print ISSN: 0021-9746
    Electronic ISSN: 1472-4146
    Topics: Medicine
    Published by BMJ Publishing Group
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  • 2
    Keywords: ASSOCIATION ; POLYMORPHISMS ; VARIANTS ; IDENTIFICATION ; METAANALYSIS ; LOCUS
    Abstract: The presence of regulatory T cells (Treg) in solid tumors is known to play a role in patient survival in ovarian cancer and other malignancies. We assessed inherited genetic variations via 749 tag single-nucleotide polymorphisms (SNP) in 25 Treg-associated genes (CD28, CTLA4, FOXP3, IDO1, IL10, IL10RA, IL15, 1L17RA, IL23A, IL23R, IL2RA, IL6, IL6R, IL8, LGALS1, LGALS9, MAP3K8, STAT5A, STAT5B, TGFB1, TGFB2, TGFB3, TGFBR1, TGRBR2, and TGFBR3) in relation to ovarian cancer survival. We analyzed genotype and overall survival in 10,084 women with invasive epithelial ovarian cancer, including 5,248 high-grade serous, 1,452 endometrioid, 795 clear cell, and 661 mucinous carcinoma cases of European descent across 28 studies from the Ovarian Cancer Association Consortium (OCAC). The strongest associations were found for endometrioid carcinoma and IL2RA SNPs rs11256497 [HR, 1.42; 95% confidence interval (CI), 1.22-1.64; P = 5.7 x 10(-6)], rs791587 (HR, 1.36; 95% CI, 1.17-1.57; P = 6.2 x 10(-5)), rs2476491 (HR, = 1.40; 95% CI, 1.19-1.64; P = 5.6 x 10(-5)), and rs10795763 (HR, 1.35; 95% CI, 1.17-1.57; P = 7.9 x 10(-5)), and for clear cell carcinoma and CTLA4 SNP rs231775 (HR, 0.67; 95% CI, 0.54-0.82; P = 9.3 x 10(-5)) after adjustment for age, study site, population stratification, stage, grade, and oral contraceptive use. The rs231775 allele associated with improved survival in our study also results in an amino acid change in CTLA4 and previously has been reported to be associated with autoimmune conditions. Thus, we found evidence that SNPs in genes related to Tregs seem to play a role in ovarian cancer survival, particularly in patients with clear cell and endometrioid epithelial ovarian cancer.
    Type of Publication: Journal article published
    PubMed ID: 24764580
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  • 3
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 129 (1989), S. 285-293 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1420-9136
    Keywords: Mining-induced seismicity ; faulting models ; peak ground parameters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Source parameter estimates based on the homogeneous and inhomogeneous source models have been examined for an anomalous sequence of seven mine-induced events located between 640 and 825 m depth at Strathcona mine, Ontario, and having magnitudes ranging betweenm N 0.8 and 2.7. The derived Brune static stress drops were found to be similar to those observed for natural earthquakes (∼30 bars), whereas dynamic stress drops were found to range up to 250–300 bars. Source radii derived from Madariaga's model better fit documented evidence of underground damage. These values of source radii were similar to those observed for the inhomogeneous model. The displacement at the source, based on the observed attenuation relationship, was about 60 mm for three magnitude 2.7 events. This is in agreement with slip values calculated using peak velocities and assuming the asperity as a Brune source within itself (72 mm). By using Madariaga's model for the asperity, the slip was over 3 times larger than observed. Peak velocity and acceleration scaling relations with magnitude were investigated by incorporating available South African data, appropriately reduced to Canadian geophysical conditions. The dynamic stress drop scaled as the square root of the seismic moment, similar to reported results in the literature for crustal earthquakes. This behavior suggests that the size of the asperities responsible for the peak ground motion, with respect to the overall source size, follow distributions that may be similar over a wide range of magnitudes. Measurements of source rupture complexity (ranging from 2 to 4) were found to agree with estimates of overall source to asperity radii, suggesting, together with the observed low rupture velocities (0.3 β to 0.6 β), that the sources were somewhat complex. Validation of source model appropriateness was achieved by direct comparison of the predicted ground motion level to observed underground damage in Creighton mine, located within the same regional stress and geological regime as Strathcona mine. Close to the source (〈100 m), corresponding to relatively higher damage levels, a good agreement was found between the predicted peak particle velocities for the inhomogeneous model and velocities derived based on established geomechanical relationships. The similarity between asperity radii and the regions of the highest observed damage provided additional support for the use of the inhomogeneous source model in the assessment of damage potential.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Geophysical prospecting 48 (2000), S. 0 
    ISSN: 1365-2478
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Physics
    Notes: The ability to analyse shear-wave anisotropy in a mine environment is greatly aided by using multiple source orientations of a reproducible, impulsive shear-wave source. The analysis of what is probably the first controlled source shear-wave experiment in a mine environment demonstrates clearly that shear-wave polarizations and time delays between split shear-wave arrivals are reliably measured because of the use of multiple source orientations rather than a single shear-wave source. Reliability is further aided by modelling the shear-wave source radiation pattern, which allows for the unequivocal discrimination between seismic raypaths where shear-wave splitting did and did not occur. The analysis also demonstrates the great importance of high reproducibility of the seismic source for the use of shear waves in time-lapse surveys to monitor changes in a rockmass.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    ISSN: 1365-2621
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Optical absorption characteristics of corn oil were investigated in the visible and near infrared regions between 400 and 2500 nm as the oil degraded during potato frying. The visible wavelength of maximum absorption between 400 and 600 nm shifted towards longer wavelengths as the frying operation progressed. The results showed that light absorption in the visible range of the electromagnetic spectrum between 400 and 600nm correlated well with viscosity.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1365-2478
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Physics
    Notes: The Shear-Wave Experiment at Atomic Energy of Canada Limited's Underground Research Laboratory was probably the first controlled-source shear-wave survey in a mine environment. Taking place in conjunction with the excavation of the Mine-by test tunnel at 420 m depth, the shear-wave experiment was designed to measure the in situ anisotropy of the rockmass and to use shear waves to observe excavation effects using the greatest variety of raypath directions of any in situ shear-wave survey to date. Inversion of the shear-wave polarizations shows that the anisotropy of the in situ rockmass is consistent with hexagonal symmetry with an approximate fabric orientation of strike 023° and dip 35°. The in situ anisotropy is probably due to microcracks with orientations governed by the in situ stress field and to mineral alignment within the weak gneissic layering. However, there is no unique interpretation as to the cause of the in situ anisotropy as the fabric orientation agrees approximately with both the orientation expected from extensive-dilatancy anisotropy and that of the gneissic layering. Eight raypaths with shear waves propagating wholly or almost wholly through granodiorite, rather than granite, do not show the expected shear-wave splitting and indicate a lower in situ anisotropy, which may be due to the finer grain size and/or the absence of gneissic layering within the granodiorite. These results suggest that shear waves may be used to determine crack and mineral orientations and for remote monitoring of a rockmass. This has potential applications in mining and waste monitoring.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1365-2621
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Water diffusivity was determined for a restructured potato product undergoing deep-fat frying. Pre-fried product gel-strength, expressed by its deformability modulus, varied from 37 to 127 kPa and markedly affected the effective diffusion coefficients, ranging from 3.31 to 1.58 × 10−9 m2 s−1, respectively. Pre- and post-fried effective water diffusivity decreased with higher deformability modulus. Frying time reduced the effective water diffusivity only when the initial deformability modulus was higher than 52 and 79 kPa for 1 and 5 min, respectively. Oil uptake criterion was found to increase with the effective water diffusivity for frying durations of 1 and 5 min.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-072X
    Keywords: Phthalate catabolism ; Nitrate respiration ; Benzoate ; Gentisate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Bacillus sp., isolated by anaerobic enrichment on a o-phthalic acid-nitrate medium, grew either aerobically or anaerobically on phthalic acid. Cells grown anaerobically on phthalate immediately oxidized phthalate and benzoate with nitrate, whereas aerobic oxidation only occurred after a lag period and was inhibited by chloramphenicol. 2-Fluoro-and 3-fluorobenzoate were formed from 3-fluorophthalate by cells grown anaerobically on phthalate. Aerobically grown cells immediately oxidized phthalate, benzoate, 3-hydroxybenzoate and gentisate with oxygen. The aerobic and anaerobic route of catabolism of phthalate may thus share an initial decarboxylation to benzoate. This is the first report of the anaerobic dissimilation of phthalic acid by a pure bacterial culture.
    Type of Medium: Electronic Resource
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